Gene transcription and steviol glycoside accumulation in <i>Stevia rebaudiana</i> under polyethylene glycol‐induced drought stress in greenhouse cultivation

Hajihashemi, Shokoofeh; Geuns, Jan

doi:10.1002/2211-5463.12099

Cited by 38 publications

(30 citation statements)

References 29 publications

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“…Furthermore, in Arabidopsis , ectopic over-expression of UGT74E2 increased the tolerance to salinity and drought stress, and reduced the water loss in plants 38 , while 11 UGTs were up-regulated in response to H 2 O 2 stress in catalase-deficient plants 39 . Recently, UGT85C2 , UGT74G1 , and UGT76G1 were shown to be downregulated under polyethylene glycol-induced drought stress in Stevia rebaudiana 40 . The role of abscisic acid (ABA) in mediating drought stress has been extensively studied and several UGTs have been functionally characterized for ABA-glucose ester formation 41 – 44 .…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic and transcriptomic analyses of Family-1 UDP glycosyltransferase in three Brassica species and Arabidopsis indicates stress-responsive regulation

Rehman

Nawaz

Shah

et al. 2018

Sci Rep

104

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In plants, UGTs (UDP-glycosyltransferases) glycosylate various phytohormones and metabolites in response to biotic and abiotic stresses. Little is known about stress-responsive glycosyltransferases in plants. Therefore, it is important to understand the genomic and transcriptomic portfolio of plants with regard to biotic and abiotic stresses. Here, we identified 140, 154, and 251 putative UGTs in Brassica rapa, Brassica oleracea, and Brassica napus, respectively, and clustered them into 14 major phylogenetic groups (A–N). Fourteen major KEGG pathways and 24 biological processes were associated with the UGTs, highlighting them as unique modulators against environmental stimuli. Putative UGTs from B. rapa and B. oleracea showed a negative selection pressure and biased gene fractionation pattern during their evolution. Polyploidization increased the intron proportion and number of UGT-containing introns among Brassica. The putative UGTs were preferentially expressed in developing tissues and at the senescence stage. Differential expression of up- and down-regulated UGTs in response to phytohormone treatments, pathogen responsiveness and abiotic stresses, inferred from microarray and RNA-Seq data in Arabidopsis and Brassica broaden the glycosylation impact at the molecular level. This study identifies unique candidate UGTs for the manipulation of biotic and abiotic stress pathways in Brassica and Arabidopsis.

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Section: Introductionmentioning

confidence: 99%

Comparative genomic and transcriptomic analyses of Family-1 UDP glycosyltransferase in three Brassica species and Arabidopsis indicates stress-responsive regulation

Rehman

Nawaz

Shah

et al. 2018

Sci Rep

104

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“…Stevia cultivation in Iran with a hot and dry weather faces some problems associated with drought stress. Previous studies have shown that S. rebaudiana was not resistant to water stress under in vitro and greenhouse conditions (Hajihashemi and Ehsanpour 2013; Hajihashemi and Geuns 2016;Hajihashemi and Ehsanpour 2014). Hence, the most practical approach for increasing Stevia yield in drought condition is to study on features involved in Stevia cell response to drought stress.…”

Section: Introductionmentioning

confidence: 99%

“…The enzymes of kaurene oxidase (KO) and kaurene synthase (KS) involve in biosynthesis of both GA and SV molecules (Brandle and Telmer 2007). It is reported that GA treatment significantly increased the transcription of ent-KS1, ent-KO in S. rebaudiana, while the GA biosynthesis inhibitor, paclobutrazol (PBZ) reduced their transcriptions (Hajihashemi and Geuns 2016;.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, high crop production under unfavorable growth condition is one major target for researches in 21 th century, the knowledge of cell response to drought stress could be used to create new varieties of crops with higher yield under water deficit. Our previous study on Stevia plants showed that PEG-induced drought stress had negative effect on SVglys biosynthesis, plant growth and yield Hajihashemi and Geuns 2016). Therefore, we decided to investigate callus of Stevia to better understand unorganized cell responses to water deficit.…”

Section: Introductionmentioning

confidence: 99%

“…In order to evaluate antioxidant system and osmolytes, the enzymes SOD, CAT and APX, total antioxidant capacity (FRAP), H 2 O 2 , proteins, proline, total amino acids and activities of proline metabolizing enzymes (pyrroline-5-carboxylate synthetase, proline dehydrogenase) were studied. Study on Stevia showed that PBZ and GA treatments could partly alleviate negative effect of PEG treatment on plant (Hajihashemi and Ehsanpour 2013, 2014; Hajihashemi and Geuns 2016). In a follow-up study, the interaction of PBZ or GA with PEG treatments was examined in which we figured out if these treatments can reduce negative effects of PEG-induced drought stress in callus.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments

Hajihashemi¹,

Rajabpoor

Djalović

2018

Physiol Mol Biol Plants

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The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l-1) and gibberellin (GA; 0 and 2 mg l-1) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H 2 O 2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG. The activity of superoxide dismutase, catalase and ascorbate peroxidase significantly increased in PEG treatment alone or in combination with PBZ and GA. All treatments of PEG, PBZ and GA significantly increased proteins, amino acids and proline contents, with the highest increase in presence of PBZ in medium culture. In contrary to proline, the activity of pyrroline-5-carboxylate synthetase and proline dehydrogenase did not change in response to any of the treatments. Collectively, our results demonstrated that PBZ and GA increased reactive oxygen species scavenging and osmolytes in PEG-treated calli more than PEG treatment alone to alleviate negative effects of PEG on Stevia calli. These findings will enable us to design effective genetic engineering strategies in callus culture to generate some somaclonal variation that may be useful in enhancing drought resistance in Stevia.

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Mathematical and intelligent modeling of stevia (Stevia Rebaudiana) leaves drying in an infrared‐assisted continuous hybrid solar dryer

Bakhshipour

Zareiforoush

Bagheri

2020

Food Science & Nutrition

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Drying characteristics of stevia leaves were investigated in an infrared (IR)‐assisted continuous‐flow hybrid solar dryer. Drying experiments were conducted at the inlet air temperatures of 30, 40, and 50°C, air inlet velocities of 7, 8, and 9 m/s, and IR lamp input powers of 0, 150, and 300 W. The results indicated that inlet air temperature and IR lamp input power had significant effect on drying time (p < .05). A comparative study was performed among mathematical, Artificial Neural Networks (ANNs), and Adaptive Neuro‐Fuzzy System (ANFIS) models for predicting the experimental moisture ratio (MR) of stevia leaves during the drying process. The ANN model was the most accurate MR predictor with coefficient of determination (R2), root mean squared error (RMSE), and chi‐squared error (χ2) values of 0.9995, 0.0005, and 0.0056, respectively, on test dataset. These values of the ANFIS model on test dataset were 0.9936, 0.0243, and 0.0202, respectively. Among the mathematical models, the Midilli model was the best‐fitted model to experimental MR values in most of the drying conditions. It was concluded that artificial intelligence modeling is an effective approach for accurate prediction of the drying kinetics of stevia leaves in the continuous‐flow IR‐assisted hybrid solar dryer.

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Gene transcription and steviol glycoside accumulation in Stevia rebaudiana under polyethylene glycol‐induced drought stress in greenhouse cultivation

Cited by 38 publications

References 29 publications

Comparative genomic and transcriptomic analyses of Family-1 UDP glycosyltransferase in three Brassica species and Arabidopsis indicates stress-responsive regulation

Comparative genomic and transcriptomic analyses of Family-1 UDP glycosyltransferase in three Brassica species and Arabidopsis indicates stress-responsive regulation

Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments

Mathematical and intelligent modeling of stevia (Stevia Rebaudiana) leaves drying in an infrared‐assisted continuous hybrid solar dryer

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