Gene Therapy with Adenoviral Plasmids or Naked DNA of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor Accelerates Healing of Duodenal Ulcer in Rats

Abstract

After we demonstrated that daily intragastric administration of angiogenic growth factors like basic fibroblast growth factor (bFGF

Cellular and Molecular Mechanisms of Gastrointestinal Ulcer Healing

“…These studies clearly proved that the local gene therapy with plasmid cDNA encoding VEGF significantly increases angiogenesis and accelerates gastric esophageal ulcer healing (51,52). Szabo and co-workers successfully applied gene therapy with adenoviral plasmids or naked DNA of VEGF and PDGF to duodenal ulcers and demonstrated that this treatment accelerates duodenal ulcer healing (53,54).…”

Improved stomach selectivity of gene expression following microinstillation of plasmid DNA onto the gastric serosal surface in mice

“…This process involves many genes encoding growth factors, including epidermal growth factor, VEGF, keratinocyte growth factor, hepatocyte growth factor, platelet-derived growth factor, basic fibroblast growth factor, and angiopoietins. It has been reported that genes encoding these growth factors have an ulcer healing effect in vivo [2,[32][33][34]. Moreover, gene therapy has been tried for gastric cancer in vitro and in vivo with various therapeutic genes, namely p53 [35], FHIT [36], NK4 [37,38] and the Fas ligand [39].…”

Pretreatment with Epidermal Growth Factor Enhances Naked Plasmid DNA Transfer onto Gastric Serosal Surface in Mice

“…It has been reported that genes encoding these growth factors have an ulcer healing effect in vivo. 2,[36][37][38] Moreover, gene therapy has been tried for gastric cancer in vitro and in vivo with various strategies, such as transfer of suicide genes, 39) the p51A gene, 40) dominant negative insulin-like growth factor I receptor gene, 41) and RhoA and RhoC short interfering RNA. 42) Efficient and targetselective gene delivery systems are important factors determining whether or not gene therapy succeeds.…”