Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery

Dobson, Jon

doi:10.1038/sj.gt.3302720

Cited by 533 publications

(341 citation statements)

References 14 publications

Supporting

Mentioning

332

Contrasting

Unclassified

Order By: Relevance

“…Stimuli-responsive polyplexes [52][53][54] react with structural alterations such as conformational changes, cleavage of chemical bonds, exposure of functional domains or disassembly (see Figure 3). In addition to physiological differences, programmed nanosystems may also be responsive to artificial stimuli as applied in physical targeting strategies including magnetic fields, 55 or light. 2,56 For example, PEG-shielded polyplexes that in the endosomal acidic environment release their PEG shield by cleavage of pHGene therapy progress and prospects D Schaffert and E Wagner labile bonds displayed improved transfection efficiency.…”

Section: Combinatorial Chemistry Allows Rapid Polymer Development Formentioning

confidence: 99%

Gene therapy progress and prospects: synthetic polymer-based systems

2008

View full text Add to dashboard Cite

Low efficiency, significant toxicity, polymer polydispersity and poorly understood delivery mechanisms have initially plagued the field of polymer-based gene therapy. Numerous strategies have helped to improve polyplexes, including the development of biodegradable polymers with reduced toxicity, incorporation of cell targeting, surface shielding and additional transport domains for effective and specific delivery, or improved chemistry for syntheses of polymers with uniform size and topology. Combined biooptical imaging and bioinformatics, providing insights into transfer bottlenecks, have helped to design improved polyplexes. Bioresponsive multifunctional polymers adapt in a dynamic manner to delivery barriers for efficient transfer of pDNA or siRNA to the target site.

show abstract

Section: Combinatorial Chemistry Allows Rapid Polymer Development Formentioning

confidence: 99%

Gene therapy progress and prospects: synthetic polymer-based systems

2008

View full text Add to dashboard Cite

show abstract

“…Several reports have been published on the use of IONPs as nanocarriers for drug and gene delivery [75]. The presence of a magnetic core off ers the promise of targeting specifi c organs within the body [76].…”

Section: Drug and Gene Deliverymentioning

confidence: 99%

The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applications

et al. 2010

View full text Add to dashboard Cite

Iron-oxide nanoparticles (IONPs) represent a significant class of inorganic nanomaterial that is contributing to the current revolution in nanomedicine [1][2]. Their unique physical properties, including high surface area to volume ratios and superparamagnetism, confer useful attributes for medical applications such as magnetic resonance imaging (MRI), drug and gene delivery, tissue engineering and bioseparation [3].Two distinct classes of superparamagnetic IONP-based materials are currently used for medical applications: superparamagnetic iron-oxide (SPIO) nanoparticles with a mean particle diameter of 50-100 nm, and ultra-small superparamagnetic iron-oxide (USPIO) nanoparticles with a size below 50 nm. Th ese two classes of IONPs have been studied widely for medical applications, particularly as the next (potential) generation of MRI contrast agents. Th ey are also seen as potential vectors for drug and gene delivery. Th e biodistribution of these nanoparticles can be altered by the application of an external magnetic fi eld; they also have potential applications in hyperthermia therapy as some magnetic particles can heat up under the infl uence of a localized high-frequency magnetic fi eld.Th e intent of this review is to present recent advances in the synthesis of IONPs and their subsequent stabilization in biological fluids using polymers, focusing on the current strategies used to graft polymers onto IONPs surfaces (see Figure 1) and the diff erent types of polymers used. Th e additional properties conferred by the polymers, such as targeting, eff ects on biodistribution and pharmacokinetics, are also discussed, and the main applications of IONPs are reviewed. Synthesis and properties of iron-oxide nanoparticlesSPIO and USPIO nanoparticles are the most extensively studied magnetic nanoparticles for biomedical applications as they are both biocompatible and easy to synthesize. Both are composed of ferrite nanocrystallites of magnetite (Fe 3 O 4 ) or maghemite (Fe 2 O 3 , γ). Over the last ten years, there has been an explosion of interest in these materials and this has been reflected in a large number of recent publications describing the synthesis and modifi cation of hybrid IONPs. In this review, we focus on the polymer modifi cation of the nanoparticles, and provide only minimal information on the inorganic synthesis of IONPs. For more detailed information on IONP synthesis, readers are referred to other reviews [3,4]. The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applicationsCyrille Boyer 1 * , Michael R. Whittaker 1 , Volga Bulmus 2 , Jingquan Liu 1 and Thomas P. Davis 1 * University of New South Wales, AustraliaOver the past decade, the synthesis of superparamagnetic nanoparticles, especially iron-oxide nanoparticles (IONPs), has been researched intensively for many high-technology applications, including enhanced storage media, biosensing and medical applications. In medicine, IONPs are used as contrast agents in magnetic resonance imaging and in hyperthermia...

show abstract

“…[164] It is postulated that DNA are released into the cytoplasm depending on the composition of the magnetic nanoparticles. [165] Magnetofection has been successfully applied to a wide range of primary cells and cells that are hard to transfect using other nonviral methods. [166] Recent work using a local injection of the nanoparticles into the gastrointestinal track and the ear vasculature [167] imply that this well accepted method for in vitro gene delivery may be applicable to in vivo gene delivery.…”

Section: Magnetofectionmentioning

confidence: 99%

Advances in Gene Delivery Systems

et al. 2011

View full text Add to dashboard Cite

The transfer of genes into cells, both in vitro and in vivo, is critical for studying gene function and conducting gene therapy. Methods that utilize viral and nonviral vectors, as well as physical approaches, have been explored. Viral vector-mediated gene transfer employs replication-deficient viruses such as retro-virus, adenovirus, adeno-associated virus and herpes simplex virus. A major advantage of viral vectors is their high gene delivery efficiency. The nonviral vectors developed so far include cationic liposomes, cationic polymers, synthetic peptides and naturally occurring compounds. These nonviral vectors appear to be highly effective in gene delivery to cultured cells in vitro but are significantly less effective in vivo. Physical methods utilize mechanical pressure, electric shock or hydrodynamic force to transiently permeate the cell membrane to transfer DNA into target cells. They are simpler than viral-and nonviral-based systems and highly effective for localized gene delivery. The past decade has seen significant efforts to establish the most desirable method for safe, effective and target-specific gene delivery, and good progress has been made. The objectives of this review are to (i) explain the rationale for the design of viral, nonviral and physical methods for gene delivery; (ii) provide a summary on recent advances in gene transfer technology; (iii) discuss advantages and disadvantages of each of the most commonly used gene delivery methods; and (iv) provide future perspectives.

show abstract

Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery

Cited by 533 publications

References 14 publications

Gene therapy progress and prospects: synthetic polymer-based systems

Gene therapy progress and prospects: synthetic polymer-based systems

The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applications

Advances in Gene Delivery Systems

Contact Info

Product

Resources

About