Gene structure, molecular characterization and transcriptional expression of two p38 isoforms ( MAPK11 and MAPK14 ) from rock bream ( Oplegnathus fasciatus )

Umasuthan, Navaneethaiyer; Bathige, S.D.N.K.; Noh, Jae Koo

doi:10.1016/j.fsi.2015.09.018

Cited by 39 publications

(15 citation statements)

References 55 publications

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“…Mitogen-activated protein kinase 14 ( MAPK14 ) encodes P38 mitogen-activated protein kinase and can be activated by various environmental stressors and pro-inflammatory cytokines (Han et al, 2015). MAPK14 regulates the activation of several transcription factors responses, including gene expression, growth, inflammation, metabolism, and apoptosis (Umasuthan et al, 2015). MAPK14 activity-deficient mice had less kidney dysfunction, inflammation, and apoptosis in acute folate nephropathy, while MAPK14 siRNA targeting decreased inflammation and cell death in cultured tubular cells (Ortiz et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Target Identification of Active Constituents of Shen Qi Wan to Treat Kidney Yang Deficiency Using Computational Target Fishing and Network Pharmacology

et al. 2019

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Background: Kidney yang deficiency syndrome (KYDS) is one of the most common syndromes treated with traditional Chinese medicine (TCM) among elderly patients. Shen Qi Wan (SQW) has been effectively used in treating various diseases associated with KYDS for hundreds of years. However, due to the complex composition of SQW, the mechanism of action remains unknown. Purpose: To identify the mechanism of the SQW in the treatment of KYDS and determine the molecular targets of SQW. Methods: The potential targets of active ingredients in SQW were predicted using PharmMapper. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out using the Molecule Annotation System (MAS3.0). The protein–protein interaction (PPI) network of these potential targets and “components-targets-pathways” interaction networks were constructed using Cytoscape. We also established a KYDS rat model induced by adenine to investigate the therapeutic effects of SQW. Body weight, rectal temperature, holding power, water intake, urinary output, blood urea nitrogen (BUN), serum creatinine (Scr), adrenocorticotrophic hormone (ACTH), cortisol (CORT), urine total protein (U-TP), and 17-hydroxy-corticosteroid (17-OHCS) were measured. Additionally, the mRNA expression levels of candidates were detected by qPCR. Results: KYDS-caused changes in body weight, rectal temperature, holding power, water intake, urinary output, BUN, Scr, ACTH, CORT, U-TP, and 17-OHCS were corrected to the baseline values after SQW treatment. We selected the top 10 targets of each component and obtained 79 potential targets, which were mainly enriched in the proteolysis, protein binding, transferase activity, T cell receptor signaling pathway, and focal adhesion. SRC , MAPK14 , HRAS , HSP90AA1 , F2 , LCK , CDK2 , and MMP9 were identified as targets of SQW in the treatment of KYDS. The administration of SQW significantly suppressed the expression of SRC , HSP90AA1 , LCK , and CDK2 and markedly increased the expression of MAPK14 , MMP9 , and F2 . However, HRAS levels remained unchanged. Conclusion: These findings demonstrated that SQW corrected hypothalamic–pituitary–target gland axis disorder in rats caused by KYDS. SRC, MAPK14 , HRAS , HSP90AA1 , F2 , LCK , CDK2 , and MMP9 were determined to the therapeutic target for the further investigation of SQW ...

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Section: Discussionmentioning

confidence: 99%

Target Identification of Active Constituents of Shen Qi Wan to Treat Kidney Yang Deficiency Using Computational Target Fishing and Network Pharmacology

et al. 2019

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“…A knock-down experiment in zebrafish showed MAPK3 and MAPK14 mRNA expression was required at an early embryonic stage (from the 2,4 cell stage to 50% epiboly) in the gilthead sea bream (Sparus aurata) [65,66]. Zygotic expression of MAPK3 and MAPK14 were upregulated by the immune challenge in several fish species [65,67]. Expression of maternal MAPK14 in M. anguillicaudatus declined around the gastrulation stage.…”

Section: Identification Of Maternal Gene Transcripts Related To Immunmentioning

confidence: 99%

Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of Misgurnus anguillicaudatus and Identification of Immune-Related Maternal Genes

Kim

Seo

et al. 2020

IJMS

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Maternal genes are important in directing early development and determining egg quality in fish. We here report the de novo transcriptome from four tissue libraries of the cyprinid loach, Misgurnus anguillicaudatus, and for the first time identified maternal gene transcripts in unfertilized eggs and suggest their immune system involvement. Expression profiles and functional enrichment revealed a total 24,116 transcripts were expressed as maternal transcripts in unfertilized eggs, which were involved in a wide range of biological functions and pathways. Comparison expression profiles and analysis of tissue specificity revealed that the large numbers of maternal transcripts were stored in unfertilized eggs near the late phase of ovarian maturation and before ovulation. Functional classification showed a total of 279 maternal immune-related transcripts classified with immune system process GO term and immune system KEGG pathway. qPCR analysis showed that transcript levels of identified maternal immune-related candidate genes were dynamically modulated during development and early ontogeny of M. anguillicaudatus. Taken together, this study could not only provide knowledge on the protective roles of maternal immune-related genes during early life stage of M. anguillicaudatus but could also be a valuable transcriptomic/genomic resource for further analysis of maternally provisioned genes in M. anguillicaudatus and other related teleost fishes.

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“…In addition, since the CM is an animal model for serious coronavirus infection [60], this may have research implications for COVID-19 vaccine and therapeutics development. Given these considerations, and that the MAPK are active in innate cell immunity [61], we believe that our research is most relevant and may potentially inform future development in preventative strategies as has been done in rats using stem cell vaccination [62] based on the "seed" hypothesis that could be applied to the "soil" counterpart based on the above findings.…”

Section: Discussionmentioning

confidence: 91%

p38γ Activation and BGP (Biliary Glycoprotein) Induction in Primates at Risk for Inflammatory Bowel Disease and Colorectal Cancer—A Comparative Study with Humans

2020

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Colorectal cancer (CRC) is a common cause of cancer-related deaths largely due to CRC liver metastasis (CRLM). Identification of targetable mechanisms continues and includes investigations into the role of inflammatory pathways. Of interest, MAPK is aberrantly expressed in CRC patients, yet the activation status is not defined. The present study assessed p38γ activation in CRC patients, cancer cells, and tissues of cotton top tamarin (CTT) and common marmoset (CM). The primate world is an overlooked resource as colitis-CRC-prone CTT are usually inure to liver metastasis while CM develop colitis but not CRC. The results demonstrate that p38γ protein and phosphorylation levels are significantly increased in CRC patients compared to normal subjects and CTT. Furthermore, p38γ phosphorylation is significantly elevated in human CRC cells and hepatoblastoma cells but not in CM colon. Additionally, carcinoembryonic antigen (CEA) and biliary glycoprotein (BGP) are induced in the CRC patients that showed p38γ phosphorylation. Inhibition of p38 MAPK in CRC cells showed a significant decline in cell growth with no effect on apoptosis or BGP level. Overall, p38γ is activated in CRC tumorigenesis and likely involves CEA antigens during CRLM in humans but not in the CTT or CM, that rarely develop CRLM.

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Gene structure, molecular characterization and transcriptional expression of two p38 isoforms ( MAPK11 and MAPK14 ) from rock bream ( Oplegnathus fasciatus )

Cited by 39 publications

References 55 publications

Target Identification of Active Constituents of Shen Qi Wan to Treat Kidney Yang Deficiency Using Computational Target Fishing and Network Pharmacology

Target Identification of Active Constituents of Shen Qi Wan to Treat Kidney Yang Deficiency Using Computational Target Fishing and Network Pharmacology

Transcriptome Analysis of Maternal Gene Transcripts in Unfertilized Eggs of Misgurnus anguillicaudatus and Identification of Immune-Related Maternal Genes

p38γ Activation and BGP (Biliary Glycoprotein) Induction in Primates at Risk for Inflammatory Bowel Disease and Colorectal Cancer—A Comparative Study with Humans

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