Gene structure and spatiotemporal expression profile of tomato genes encoding YUCCA-like flavin monooxygenases: The ToFZY gene family

Expósito-Rodríguez, Marino; Borges, Andrés A.; Borges-Pérez, Andrés; Pérez, Jose Antonio Pérez

doi:10.1016/j.plaphy.2011.02.022

Cited by 57 publications

(55 citation statements)

References 52 publications

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“…10B). ToFZY1 and ToFZY6 are highly expressed in young tomato fruits (Expósito-Rodríguez et al, 2011). LMHS reduced the transcript levels of ToFZY1 in 2-dbp ovaries and ToFZY6 in 2-dap fruits in the wild type but not in the CWIN-elevated transgenic plants (Fig.…”

Section: Cwin-elevated Transgenic Tomato Exhibited a Sustained Or Incmentioning

confidence: 84%

“…This analysis includes two auxin-responsive genes (IAA9 and ARF7) and two FZY genes (ToFZY1 and ToFZY6). IAA9 and ARF7 are transcription factors negatively regulating the auxin response and signaling during tomato fruit set (Wang et al, 2005;De Jong et al, 2009), whereas the two tomato FZY genes are YUCCA-like genes encoding flavin monooxygenases, which are rate-limiting enzymes in the synthesis of indole-3-acetic acid (IAA; Expósito-Rodríguez et al, 2011). The analyses revealed a significantly lower level of IAA9 mRNA in transgenic tomato than that in the wild type in 2-dbp ovaries and 2-dap fruits under both control and LMHS conditions (Fig.…”

Section: Cwin-elevated Transgenic Tomato Exhibited a Sustained Or Incmentioning

confidence: 99%

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Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

2016

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Reduced cell wall invertase (CWIN) activity has been shown to be associated with poor seed and fruit set under abiotic stress. Here, we examined whether genetically increasing native CWIN activity would sustain fruit set under long-term moderate heat stress (LMHS), an important factor limiting crop production, by using transgenic tomato (Solanum lycopersicum) with its CWIN inhibitor gene silenced and focusing on ovaries and fruits at 2 d before and after pollination, respectively. We found that the increase of CWIN activity suppressed LMHS-induced programmed cell death in fruits. Surprisingly, measurement of the contents of H 2 O 2 and malondialdehyde and the activities of a cohort of antioxidant enzymes revealed that the CWINmediated inhibition on programmed cell death is exerted in a reactive oxygen species-independent manner. Elevation of CWIN activity sustained Suc import into fruits and increased activities of hexokinase and fructokinase in the ovaries in response to LMHS. Compared to the wild type, the CWIN-elevated transgenic plants exhibited higher transcript levels of heat shock protein genes Hsp90 and Hsp100 in ovaries and HspII17.6 in fruits under LMHS, which corresponded to a lower transcript level of a negative auxin responsive factor IAA9 but a higher expression of the auxin biosynthesis gene ToFZY6 in fruits at 2 d after pollination. Collectively, the data indicate that CWIN enhances fruit set under LMHS through suppression of programmed cell death in a reactive oxygen species-independent manner that could involve enhanced Suc import and catabolism, HSP expression, and auxin response and biosynthesis.

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Section: Cwin-elevated Transgenic Tomato Exhibited a Sustained Or Incmentioning

confidence: 84%

Section: Cwin-elevated Transgenic Tomato Exhibited a Sustained Or Incmentioning

confidence: 99%

Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

2016

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“…YUC genes have been identified in all of the sequenced plant genomes, suggesting that YUCs are involved in an evolutionarily conserved auxin biosynthesis pathway. It has been shown that overexpression of YUC genes in Arabidopsis (Zhao et al, 2001;Cheng et al, 2006;Kim et al, 2007;Hentrich et al, 2013;), tomato (Exposito-Rodriguez et al, 2011), tobacco (Zhao et al, 2001), petunia (Tobena-Santamaria et al, 2002), potato (Kim et al, 2012), strawberry , and rice (Yamamoto et al, 2007) leads to auxin overproduction.…”

Section: Identification Of Yucca Genes By Activation Taggingmentioning

confidence: 99%

Auxin Biosynthesis

Zhao

2014

The Arabidopsis Book

188

171

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Indole-3-acetic acid (IAA), the most important natural auxin in plants, is mainly synthesized from the amino acid tryptophan (Trp). Recent genetic and biochemical studies in Arabidopsis have unambiguously established the first complete Trp-dependent auxin biosynthesis pathway. The first chemical step of auxin biosynthesis is the removal of the amino group from Trp by the TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) family of transaminases to generate indole-3-pyruvate (IPA). IPA then undergoes oxidative decarboxylation catalyzed by the YUCCA (YUC) family of flavin monooxygenases to produce IAA. This two-step auxin biosynthesis pathway is highly conserved throughout the plant kingdom and is essential for almost all of the major developmental processes. The successful elucidation of a complete auxin biosynthesis pathway provides the necessary tools for effectively modulating auxin concentrations in plants with temporal and spatial precision. The progress in auxin biosynthesis also lays a foundation for understanding polar auxin transport and for dissecting auxin signaling mechanisms during plant development.

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“…The YUCCA-like gene family sequences were aligned by ClustalW (Thompson et al, 1994) in BioEdit (Hall, 1999) and the occurrence of the already described conserved amino acid motifs characteristic of the flavin monooxygenase gene family was determined (Expósito-Rodríguez et al, 2011;Expósito-Rodríguez et al, 2007).…”

Section: Characterization Of the Yucca-like Gene Familymentioning

confidence: 99%

Auxin Is Rapidly Induced by Herbivore Attack and Regulates a Subset of Systemic, Jasmonate-Dependent Defenses

et al. 2016

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Plant responses to herbivore attack are regulated by phytohormonal networks. To date, the role of the auxin indole-3-acetic acid (IAA) in this context is not well understood. We quantified and manipulated the spatiotemporal patterns of IAA accumulation in herbivore-attacked Nicotiana attenuata plants to unravel its role in the regulation of plant secondary metabolism. We found that IAA is strongly, rapidly, and specifically induced by herbivore attack. IAA is elicited by herbivore oral secretions and fatty acid conjugate elicitors and is accompanied by a rapid transcriptional increase of auxin biosynthetic YUCCA-like genes. IAA accumulation starts 30 to 60 s after local induction and peaks within 5 min after induction, thereby preceding the jasmonate (JA) burst. IAA accumulation does not require JA signaling and spreads rapidly from the wound site to systemic tissues. Complementation and transport inhibition experiments reveal that IAA is required for the herbivore-specific, JA-dependent accumulation of anthocyanins and phenolamides in the stems. In contrast, IAA does not affect the accumulation of nicotine or 7-hydroxygeranyllinalool diterpene glycosides in the same tissue. Taken together, our results uncover IAA as a rapid and specific signal that regulates a subset of systemic, JA-dependent secondary metabolites in herbivore-attacked plants.

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Gene structure and spatiotemporal expression profile of tomato genes encoding YUCCA-like flavin monooxygenases: The ToFZY gene family

Cited by 57 publications

References 52 publications

Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

Cell Wall Invertase Promotes Fruit Set under Heat Stress by Suppressing ROS-Independent Cell Death

Auxin Biosynthesis

Auxin Is Rapidly Induced by Herbivore Attack and Regulates a Subset of Systemic, Jasmonate-Dependent Defenses

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