Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease

Singh, Swati; Kaushal, Ankur; Gupta, Sunil; Kumar, Ashok

doi:10.1007/s12088-016-0620-6

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…Biosensor based disease diagnosis with higher sensitivity and specificity becoming the future of disease diagnosis. It is becoming a strong point of care diagnosis system for hasty and precise diagnosis of several diseases that are not facile to diagnose by other laboratory diagnosis methods (Kaushal et al 2017 ; Gupta et al 2017 ; Singh et al 2017a , b ). Biosensors composed of biospecific ligands, antibodies, nucleic acid receptors (Fig.…”

Section: Future Of Scrub Typhus Diagnosismentioning

confidence: 99%

Diagnosis of scrub typhus: recent advancements and challenges

et al. 2020

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Scrub typhus is a mite-borne, acute febrile illness caused by the bacterium Orientia tsutsugamushi. It is a re-emerging infectious disease of the tsutsugamushi triangle. Scrub typhus is transmitted through bites of contaminated chiggers (larval stage). Diagnosis of scrub typhus is challenging as its symptoms mimic with other acute febrile illnesses. Several methods are effectual for diagnosis of scrub typhus that includes enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay (IFA), immunochromatographic test (ICT), Weil-Felix, polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). Weil-Felix test was initially used for the diagnosis of scrub typhus in underdeveloped countries but not preferred due to a lack of both specificity and sensitivity. Other immuno-based methods like IFA and ELISA are most outrank for detection of scrub typhus due to their higher sensitivity and specificity, but not vigorous to lay bare the infection at early stages and need the convalescent sampling for verification of positive samples. On another deed, PCR based methods becoming acceptable over era due to its dexterity of early-stage diagnosis with higher specificity and sensitivity but lack its applicability in circumstances of scrub typhus due to the variegated genetic makeup of Orientia tsutsugamushi among its serotypes. The present review focused on various detection methods along with their advantages and disadvantages used in the diagnosis of scrub typhus. A comparison between available methods of diagnosis with challenges in the detection of scrub typhus is also summarized.

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Section: Future Of Scrub Typhus Diagnosismentioning

confidence: 99%

Diagnosis of scrub typhus: recent advancements and challenges

et al. 2020

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“…When the cloned gene sample has a complementary DNA sequence representing a target for a pre-immobilized probe, a hybridization reaction takes place and results in dsDNA at the surface of the working electrode. A redox indicator methylene blue (MB) was used for the electrochemical study (Dash et al 2012 ; Singh et al 2017 ). This study is explains a non-infectious way to develop a detection method with the help of non-infectious microbes.…”

Section: Introductionmentioning

confidence: 99%

Development of amperometric biosensor based on cloned hemagglutinin gene of H1N1 (swine flu) virus

et al. 2022

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The recent emergence of respiratory viruses especially COVID-19 and swine flu has underscored the need for robust and bedside detection methods. Swine flu virus is a very infectious virus of the respiratory system. Timely detection of this virus with high specificity and sensitivity is crucial for reducing morbidity as well as mortality. Cloning of gene segments into a non-infectious agent helps in the development of detection methods, vaccine development, and other studies. In this study, cloning was used to develop a biosensor for H1N1 pdm09 detection. A segment of the hemaglutinin gene was cloned in a vector and characterized with the help of colony touch PCR and blue–white screening. The recombinant plasmid was extracted, and the gene segment was confirmed with the help of HA-specific primers. A 5′ amine group-attached hemagglutinin (HA) gene-specific DNA probe was immobilized on the working gold electrode surface to make a quick, specific, reliable, and sensitive detection method for H1N1pdm09 virus in human nasal swab samples. The HA probe was immobilized on the cysteine applied gold electrode of the screen-printed electrode through 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N -hydroxysuccinimide (NHS). Differential pulse voltammetry was performed with the help of methylene blue, which is a redox indicator for the detection of single-stranded cloned HA gene segment. The developed sensor depicted high sensitivity for the H1N1 influenza virus with a detection limit of 0.6 ng ssDNA/6 µl of the cloned HA sample. Specificity was also checked using H3N2 virus, N. meningitides , influenza A and positive H1N1pdm09 samples.

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“…It causes infections of impetigo, scarlatina, pharyngitis and also leads to necrotizing fasciitis and sepsis diseases [2,3]. Kalia et al have several publications on genome based approach to identify specific biomarkers for identification of different strains of Streptococcus and many other bacterial pathogens [4][5][6]. One major protein streptococcal cysteine proteinase involve in the manifestation of infection-SpeB, streptococcal erythrogenic toxin B.…”

Section: Introductionmentioning

confidence: 99%

Nano-Au/cMWCNT Modified speB Gene Specific Amperometric Sensor for Rapidly Detecting Streptococcus pyogenes causing Rheumatic Heart Disease

et al. 2017

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A specific 5 0 NH 2 labeled DNA probe of speB gene was immobilized onto the gold nanoparticles/carboxylated multi walled carbon nanotubes (Nano-Au/ cMWCNT) screen printed electrode using EDC/NHS cross linking chemistry. This was followed by hybridization with 0.5-50 ng/6 ll of single stranded genomic DNA Streptococcus pyogenes infected patient throat swab samples. Electrochemical amperometric assay was deciphered by using cyclic voltammetry (CV) with methylene blue a redox indicator. The sensor had a sensitivity of 104.7 lA cm -2 ng -1 using CV with a R 2 of 0.907 and 0.01 ng/6 ll as the limit of detection (LOD). The modified electrode surface morphology was characterized using scanning electron microscopy. The stability of the electrode was seen at 4°C for 180 days having 6% loss in the initial current. The sensor is speB gene specific and can detect the pathogen within 30 min.

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Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease

Cited by 5 publications

References 21 publications

Diagnosis of scrub typhus: recent advancements and challenges

Diagnosis of scrub typhus: recent advancements and challenges

Development of amperometric biosensor based on cloned hemagglutinin gene of H1N1 (swine flu) virus

Nano-Au/cMWCNT Modified speB Gene Specific Amperometric Sensor for Rapidly Detecting Streptococcus pyogenes causing Rheumatic Heart Disease

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