Gene SNQ2 of Saccharomyces cerevislae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases

Servos, Jörg; Haase, Eckard; Brendel, Martin

doi:10.1007/bf00277115

Cited by 188 publications

(141 citation statements)

References 38 publications

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“…Of the 16 genes that have previously been shown to be controlled by one or more of the five transcription factors, we counted six transporters, of which four were members of the ATP-binding cassette (ABC) transporter, PDR5 (Balzi et al, 1994), PDR10 (Wolfger et al, 1997), PDR15 (Wolfger et al, 2004) SNQ2 (Servos et al, 1993), and the remaining two were member of the major facilitator superfamilies TPO1 (Tomitori et al, 1999) and FLR1 (Alarco et al, 1997). The 11 remaining genes that were identified in this search do not encode multidrug resistance transporters but have other or still unknown functions.…”

Section: Carotenoid Production Induces Expression Of Genes Involved Imentioning

confidence: 99%

Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response

Verwaal

Jiang

Wang

et al. 2010

Yeast

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To obtain insight into the genome-wide transcriptional response of heterologous carotenoid production in Saccharomyces cerevisiae, the transcriptome of two different S. cerevisiae strains overexpressing carotenogenic genes from the yeast Xanthophyllomyces dendrorhous grown in carbon-limited chemostat cultures was analysed. The strains exhibited different absolute carotenoid levels as well as different intermediate profiles. These discrepancies were further sustained by the difference of the transcriptional response exhibited by the two strains. Transcriptome analysis of the strain producing high carotenoid levels resulted in specific induction of genes involved in pleiotropic drug resistance (PDR). These genes encode ABC-type and major facilitator transporters which are reported to be involved in secretion of toxic compounds out of cells. β-Carotene was found to be secreted when sunflower oil was added to the medium of S. cerevisiae cells producing high levels of carotenoids, which was not observed when added to X. dendrorhous cells. Deletion of pdr10, one of the induced ABC transporters, decreased the transformation efficiency of a plasmid containing carotenogenic genes. The few transformants that were obtained had decreased growth rates and lower carotenoid production levels compared to a pdr5 deletion and a reference strain transformed with the same genes. Our results suggest that production of high amounts of carotenoids in S. cerevisiae leads to membrane stress, in which Pdr10 might play an important role, and a cellular response to secrete carotenoids out of the cell.

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Section: Carotenoid Production Induces Expression Of Genes Involved Imentioning

confidence: 99%

Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response

Verwaal

Jiang

Wang

et al. 2010

Yeast

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“…The best characterized plasma membrane members of the PDR-subfamily are the related ABC transporters Pdr5p [9] and Snq2p [10]. Overexpression of Pdr5p and Snq2p leads to PDR, since they extrude hundreds of structurally and functionally unrelated xenobiotics across the plasma membrane [10,11].…”

Section: Plasma Membrane Abc Transporters -The First Defense Linementioning

confidence: 99%

Yeast ABC transporters – A tale of sex, stress, drugs and aging

2005

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Yeast ATP-binding cassette (ABC) proteins are implicated in many biological phenomena, often acting at crossroads of vital cellular processes. Their functions encompass peptide pheromone secretion, regulation of mitochondrial function, vacuolar detoxification, as well as pleiotropic drug resistance and stress adaptation. Because yeast harbors several homologues of mammalian ABC proteins with medical importance, understanding their molecular mechanisms, substrate interaction and three-dimensional structure of yeast ABC proteins might help identifying new approaches aimed at combating drug resistance or other ABC-mediated diseases. This review provides a comprehensive discussion on the functions of the ABC protein family in the yeast Saccharomyces cerevisiae.

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“…A homology search using the BLAST program (Altschul et al, 1997) showed the closest homologs of NpABC1 to be a putative ABC transporter (AC013453) from Arabidopsis (70% identity) and TUR2 from S. polyrrhiza (69% identity; Smart and Fleming, 1996). In fungi, the closest homologs are PDR5 (28% identity; Balzi et al, 1994) and SNQ2 (25% identity; Servos et al, 1993) from S. cerevisiae .…”

Section: Isolation and Characterization Of Npabc1mentioning

confidence: 99%

A Plant Plasma Membrane ATP Binding Cassette–Type Transporter Is Involved in Antifungal Terpenoid Secretion

et al. 2001

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ATP binding cassette (ABC) transporters, which are found in all species, are known mainly for their ability to confer drug resistance. To date, most of the ABC transporters characterized in plants have been localized in the vacuolar membrane and are considered to be involved in the intracellular sequestration of cytotoxins. Working on the assumption that certain ABC transporters might be involved in defense metabolite secretion and their expression might be regulated by the concentration of these metabolites, we treated a Nicotiana plumbaginifolia cell culture with sclareolide, a close analog of sclareol, an antifungal diterpene produced at the leaf surface of Nicotiana spp; this resulted in the appearance of a 160-kD plasma membrane protein, which was partially sequenced. The corresponding cDNA ( NpABC1 ) was cloned and shown to encode an ABC transporter. In vitro and in situ immunodetection showed NpABC1 to be localized in the plasma membrane. Under normal conditions, expression was found in the leaf epidermis. In cell culture and in leaf tissues, NpABC1 expression was strongly enhanced by sclareolide and sclareol. In parallel with NpABC1 induction, cells acquired the ability to excrete a labeled synthetic sclareolide derivative. These data suggest that NpABC1 is involved in the secretion of a secondary metabolite that plays a role in plant defense. ń INTRODUCTIONThe ATP binding cassette (ABC) superfamily of membrane transporters is found in all prokaryotic and eukaryotic species. All members of this family are involved in the active transport of many chemically and structurally unrelated compounds and use ATP hydrolysis as a source of energy (Higgins, 1992). Several of these proteins have been identified by their ability to confer drug resistance, hence their designation as multidrug resistance (MDR) or pleiotropic drug resistance (PDR) proteins. For example, the human MDR1 (Chen et al., 1986), which is also designated P-glycoprotein, is implicated in resistance of cell lines to antitumor drugs (Ueda et al., 1987), PDR5 is a multidrug transporter found in Saccharomyces cerevisiae (Balzi et al., 1994), and Candida PDR1 (CDR1) and CDR2, identified in the yeast Candida albicans , contribute to fungicide resistance (Prasad et al., 1995;Sanglard et al., 1997). An analog of human MDR1 also has been found in prokaryotes (van Veen et al., 1996). Other ABC transporters were characterized by their ability to secrete internal compounds; this is the case with the S. cerevisiae protein STE6, which is involved in the transport of a mating pheromone peptide (Kuchler et al., 1989), and mammalian MDR2, which acts as a phospholipid translocator (Ruetz and Gros, 1994).Multidrug resistance-related proteins (MRPs) are ABC proteins that can be distinguished from the proteins described above by their possession of an N-terminal hydrophobic extension and an internal regulatory domain. In mammals, the MRPs HmMRP1 (Cole et al., 1992) and cMOAT (Buchler et al., 1996) transport glutathione S -conjugates and are implicated in the de...

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Gene SNQ2 of Saccharomyces cerevislae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases

Cited by 188 publications

References 38 publications

Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response

Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response

Yeast ABC transporters – A tale of sex, stress, drugs and aging

A Plant Plasma Membrane ATP Binding Cassette–Type Transporter Is Involved in Antifungal Terpenoid Secretion

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