Gene silencing results in instability of antibody production in transgenic plants

Neve, Myriam De; Buck, Sylvie De; Wilde, Chris De; Houdt, Helena Van; Strobbe, I; Jacobs, Anni; Montagu, Marc Van; Depicker, Anna

doi:10.1007/s004380050932

Cited by 56 publications

(39 citation statements)

References 42 publications

(45 reference statements)

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“…This is the first published investigation on cotton reporting the production of a large number (82) of transgenic lines that were subjected to systematic analysis to study the transgene expression levels in different lines and then monitor the in several plant species (Caligari et al, 1993;De Neve et al, 1999;James et al, 2002;Vain et al, 2002). Our results add to the growing literature indicating the importance of screening a large number of independent transformation events to identify lines with desired levels of transgene expression and then monitoring the stability of expression over several generations .…”

Section: Discussionmentioning

confidence: 99%

Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens

Emani

García

Lopata-Finch

et al. 2003

Plant Biotechnology Journal

136

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SummaryMycoparasitic fungi are proving to be rich sources of antifungal genes that can be utilized to genetically engineer important crops for resistance against fungal pathogens. Homozygous T 2 plants of the high endochitinase-expressing cotton lines were tested for disease resistance against a soil-borne pathogen, Rhizoctonia solani and a foliar pathogen, Alternaria alternata . Transgenic cotton plants showed significant resistance to both pathogens.

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Section: Discussionmentioning

confidence: 99%

Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens

Emani

García

Lopata-Finch

et al. 2003

Plant Biotechnology Journal

136

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“…2 and 4; Table 1). De Neve et al (1999) have described a similar trans silencing eect of one transgene on another based on a 204-nucleotide identity in the 3¢UTR.…”

Section: Discussionmentioning

confidence: 99%

Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism

Houdt¹,

Montagu²,

Depicker³

2000

Mol Gen Genet

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Two stable transgenic tobacco lines were obtained as segregants from a primary transformant. Plants homozygous for a T-DNA inverted repeat locus (HOlo1) showed posttranscriptional gene silencing (PTGS) of the neomycin phosphotransferase II (nptII) transgenes, whereas HOlo2 plants, homozygous for a single T-DNA insert, expressed the nptII genes normally. Transient expression of nptII genes newly introduced into leaves of both the HOlo2 and nptII-silenced HOlo1 plants was downregulated only in the silenced background. Different chimeric beta-glucuronidase (gus) genes with parts of the nptII transgene inserted in sense or antisense orientation into the 3'-untranslated region, which encoded transcripts that had homology or complementarity to nptII transcripts. showed reduced transient expression specifically in nptII-silenced tissue. Therefore, we conclude that RNAs of both polarities are targets for PTGS-induced RNA degradation, which supports the notion that double-stranded RNA acts as an inducing signal for silencing.

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“…Either the GUS expression could significantly change or the epigenetic status of the 35S-GUS transgenes in the original T-DNA locus could be maintained (De Neve et al, 1999;Fojtová et al, 2006), resulting in similar transgene expression levels of the parental locus and the resolved locus. The three F2 progenies that contained only one T-DNA copy displayed high and stable transgene expression levels.…”

Section: Discussionmentioning

confidence: 99%

Generation of Single-Copy T-DNA Transformants in Arabidopsis by the CRE/loxP Recombination-Mediated Resolution System

et al. 2007

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We investigated whether complex T-DNA loci, often resulting in low transgene expression, can be resolved efficiently into single copies by CRE/loxP-mediated recombination. An SB-loxP T-DNA, containing two invertedly oriented loxP sequences located inside and immediately adjacent to the T-DNA border ends, was constructed. Regardless of the orientation and number of SB-loxP-derived T-DNAs integrated at one locus, recombination between the outermost loxP sequences in direct orientation should resolve multiple copies into a single T-DNA copy. Seven transformants with a complex SB-loxP locus were crossed with a CRE-expressing plant. In three hybrids, the complex T-DNA locus was reduced efficiently to a single-copy locus. Upon segregation of the CRE recombinase gene, only the simplified T-DNA locus was found in the progeny, demonstrating DNA had been excised efficiently in the progenitor cells of the gametes. In the two transformants with an inverted T-DNA repeat, the T-DNA resolution was accompanied by at least a 10-fold enhanced transgene expression. Therefore, the resolution of complex loci to a single-copy T-DNA insert by the CRE/loxP recombination system can become a valuable method for the production of elite transgenic Arabidopsis thaliana plants that are less prone to gene silencing.

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Gene silencing results in instability of antibody production in transgenic plants

Cited by 56 publications

References 42 publications

Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens

Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens

Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism

Generation of Single-Copy T-DNA Transformants in Arabidopsis by the CRE/loxP Recombination-Mediated Resolution System

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