Gene silencing of TNF-alpha in a murine model of acute colitis using a modified cyclodextrin delivery system

McCarthy, Joanna; O’Neill, Martin J.; Bourré, Ludovic; Walsh, D.; Quinlan, Aoife; Hurley, Gráinne; Ogier, Julien; Shanahan, Fergus; Melgar, Silvia; Darcy, Raphael; O’Driscoll, Caitriona M.

doi:10.1016/j.jconrel.2013.03.004

Cited by 64 publications

(71 citation statements)

References 39 publications

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“…The resulting two-phase system was briefly sonicated in a bath-type sonicator (NEY 28H, USA: 120 W, 45 kHz) under a nitrogen atmosphere until the mixture forms a homogeneous opalescent dispersion. Liposomes consisting of only SO 3 -CDC 16 were purified by column chromatography on a Sephadex G-50 column (30 cm × 1.5 cm i.d.) and eluted with pure water.…”

Section: Preparation Of Liposomes By Reverse-phase Evaporationmentioning

confidence: 99%

“…DPPC and cholesterol were added to SO 3 -CDC 16 to increase the stability of the liposomes. The mole ratio of DPPC, cholesterol, and SO 3 -CDC 16 10-12 in the liposomes was 100:10:4.…”

Section: Preparation Of Liposomes By Reverse-phase Evaporationmentioning

confidence: 99%

“…Herein, r was 1.02. Figure 1 shows the synthetic method for SO 3 -α-, β-, and γ-CDC 16 . First, selective silylation at the primary hydroxyl groups of α-, β-, and γ-CD was carried out with tert-butyldimethylsilyl chloride (TBDMS-Cl) according to the method described by Ashton et al [37] to give silylated α-, β-, and γ-CD derivatives 1-3, respectively.…”

Section: Determination Of Calcein Loading and Releasing Amountsmentioning

confidence: 99%

“…In a previous paper, we reported the synthesis of 6-O-sulfated CD amphiphiles (SO 3 -CDC 16 ) and preliminary data on the formation of liposomes containing these CD derivatives [34]. Herein, the miscibility of monolayers composed of SO 3 -CDC 16 with dipalmitoyl phosphatidylcholine (DPPC) and cholesterol was investigated in detail.…”

Section: Introductionmentioning

confidence: 99%

“…Herein, the miscibility of monolayers composed of SO 3 -CDC 16 with dipalmitoyl phosphatidylcholine (DPPC) and cholesterol was investigated in detail. Moreover, the formation of these liposomes was confirmed by transition electron microscopy (TEM) using negative staining.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Sulfated Cyclodextrin Amphiphiles with Liposomal Encapsulation Properties

Furuike¹,

Nasu²,

Tamura³

2015

JEAS

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A novel class of amphiphiles with sulfate groups at the C-6 position and palmitoyl groups at the C-2, 3 positions of α-, β-, and γ-cyclodextrin (CD) were efficiently synthesized. These compounds formed stable monolayers with high collapse pressures at the air-water interface. The mixed monolayer behaviors of the 6-O-sulfated CD amphiphiles (SO3-CDC16) in the presence of dipalmitoyl phosphatidylcholine (DPPC) and cholesterol were discussed using the surface pressure-molecular area (π-A) isotherms. The collapse pressures showed maxima at molar ratios of SO3-CDC16 lower than 10 mol%. A morphological analysis of the liposomes containing DPPC and 4 mol% SO3-CDC16 formed in PBS was carried out using transmission electron microscopy with negative staining, and vesicles with maximum diameters of 350 -500 nm were observed. Moreover, the releasing ability of these liposomes was examined using a fluorescent compound, calcein. It was clearly shown that liposomes containing SO3-CDC16 could release encapsulated calcein more easily than liposomes consisting only of DPPC, and that the release rate depended on the phase transition temperature of the SO3-CDC16 included in the liposome membrane.

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Section: Preparation Of Liposomes By Reverse-phase Evaporationmentioning

confidence: 99%

“…DPPC and cholesterol were added to SO 3 -CDC 16 to increase the stability of the liposomes. The mole ratio of DPPC, cholesterol, and SO 3 -CDC 16 10-12 in the liposomes was 100:10:4.…”

Section: Preparation Of Liposomes By Reverse-phase Evaporationmentioning

confidence: 99%

Section: Determination Of Calcein Loading and Releasing Amountsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Synthesis of Sulfated Cyclodextrin Amphiphiles with Liposomal Encapsulation Properties

Furuike¹,

Nasu²,

Tamura³

2015

JEAS

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Delivering Nucleic‐Acid Based Nanomedicines on Biomaterial Scaffolds for Orthopedic Tissue Repair: Challenges, Progress and Future Perspectives

et al. 2016

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As well as acting to fill defects and allow for cell infiltration and proliferation in regenerative medicine, biomaterial scaffolds can also act as carriers for therapeutics, further enhancing their efficacy. Drug and protein delivery on scaffolds have shown potential, however, supraphysiological quantities of therapeutic are often released at the defect site, causing off-target side effects and cytotoxicity. Gene therapy involves the introduction of foreign genes into a cell in order to exert an effect; either replacing a missing gene or modulating expression of a protein. State of the art gene therapy also encompasses manipulation of the transcriptome by harnessing RNA interference (RNAi) therapy. The delivery of nucleic acid nanomedicines on biomaterial scaffolds - gene-activated scaffolds -has shown potential for use in a variety of tissue engineering applications, but as of yet, have not reached clinical use. The current state of the art in terms of biomaterial scaffolds and delivery vector materials for gene therapy is reviewed, and the limitations of current procedures discussed. Future directions in the clinical translation of gene-activated scaffolds are also considered, with a particular focus on bone and cartilage tissue regeneration.

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Oral Delivery of Biologics for Precision Medicine

2019

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Precision medicine has led to the identification of new biological drugs and targets for personalized treatments. A limitation of biological drugs relies on their difficulties for overcoming biological barriers. However, recent developments on drug delivery are opening new avenues that may soon make feasible the oral administration of biologics. In article number 1901935, María José Alonso and co‐workers provide an overview of the current situation, future prospects, barriers to clinical translation, and identified opportunities for advancement in this field.

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Gene silencing of TNF-alpha in a murine model of acute colitis using a modified cyclodextrin delivery system

Cited by 64 publications

References 39 publications

Synthesis of Sulfated Cyclodextrin Amphiphiles with Liposomal Encapsulation Properties

Synthesis of Sulfated Cyclodextrin Amphiphiles with Liposomal Encapsulation Properties

Delivering Nucleic‐Acid Based Nanomedicines on Biomaterial Scaffolds for Orthopedic Tissue Repair: Challenges, Progress and Future Perspectives

Oral Delivery of Biologics for Precision Medicine

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