Gene silencing of stearoyl-ACP desaturase enhances the stearic acid content in Chlamydomonas reinhardtii

Jaeger, Lenny de; Springer, Jan; Wolbert, E.J.H.; Martens, Dirk E.; Eggink, Gerrit; Wijffels, René H.

doi:10.1016/j.biortech.2017.06.128

Cited by 19 publications

(7 citation statements)

References 46 publications

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“…The increase in oleic acid upon nitrogen starvation was also reported for different Nannochloropsis species (Rodolfi et al 2009;Simionato et al 2013;Xiao et al 2013). Silencing stearoyl-ACP desaturase (Δ9-desaturase), which converts stearic acid into oleic acid, resulted in an increased stearic acid content in TAG (de Jaeger et al 2017). The abundance of stearoyl-ACP desaturase proteins was decreased under initial and long term nitrogen depletion in N. oceanica (Dong et al 2013).…”

Section: Eicosapentaenoic Acid (Epa) Synthesismentioning

confidence: 54%

Time-dependent transcriptome profile of genes involved in triacylglycerol (TAG) and polyunsaturated fatty acid synthesis in Nannochloropsis gaditana during nitrogen starvation

et al. 2020

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In this research, the gene expression of genes involved in lipid metabolism of the eustigmatophyte alga Nannochloropsis gaditana was measured by transcriptomic data. This microalga can be used as a source of triacylglycerol (TAG) and the omega-3 fatty acid eicosapentaenoic acid (EPA). Insight in TAG and EPA production and regulation are needed to improve their productivity. Nitrogen starvation induces TAG accumulation in N. gaditana. Previous research showed that during nitrogen starvation, EPA was translocated from the polar lipids to TAG and de novo synthesized in N. gaditana. Therefore, the expression levels of genes involved in fatty acid translocation and de novo TAG synthesis were measured. Furthermore, the genes involved in de novo EPA synthesis such as elongases and desaturases were studied. The expression levels were measured during the first hours of nitrogen starvation and the subsequent period of 14 days. One phospholipid:diacylglycerol acyltransferase (PDAT) gene involved in translocation of fatty acids from membrane lipids to TAG was upregulated. In addition, several lipases were upregulated, suggesting that these enzymes might be responsible for the translocation of EPA to TAG. Most desaturases and elongases involved in de novo EPA synthesis were downregulated during nitrogen starvation, except for Δ9 desaturase which was upregulated. This upregulation correlates with the increase in oleic acid. Due to the presence of many hypothetical genes, improvement in annotation is needed to increase our understanding of these pathways and their regulation.

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Section: Eicosapentaenoic Acid (Epa) Synthesismentioning

confidence: 54%

Time-dependent transcriptome profile of genes involved in triacylglycerol (TAG) and polyunsaturated fatty acid synthesis in Nannochloropsis gaditana during nitrogen starvation

et al. 2020

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“…Although oleic acid content showed no signi cant changes in CHL-2220, it signi cantly elevated from 7.64% to 17.09% in CHL-2221. This suggests the accumulation of TAG as oleic acid as it is one of the major constituents of triacylglycerols (TAGs) [44]. Also, an increased level of oleic acid in response to salinity stress was detected in green microalgae [45].…”

Section: Effect Of Tobacco Smoke On Fatty Acid Pro Lementioning

confidence: 94%

Effect of tobacco smoke on the growth, biochemical composition, and biodiesel characteristics of two Chlamydomonas strains

Barati

Zafar

Wang

et al. 2021

Preprint

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Microalgae has been known as one of the efficient biological carbon dioxide (CO2) sequesters. In recent years, they have been applied to improve the air quality by reducing CO2 concentration and boosting oxygen (O2) level, and have the ability to tolerate diverse toxic elements in the air. The current study investigates the effect of tobacco smoke on the growth, biochemical, and biodiesel characteristics of two Chlamydomonas strains. For this purpose, strains were exposed to seven days of tobacco smoke by burning one cigarette per hour in a closed box containing the air supply. CHL-2220 continued its proliferation without demonstrating any inhibition, while the growth of the other strain (CHL-2221) was inhibited severely. Tobacco smoke significantly altered the biochemical composition of CHL-2221, and caused the accumulation of more carotenoids and carbohydrates as part of the stress response. The analyzed biodiesel parameters presented poor quality from the extracted lipid of both strains. However, the fatty acid profile of the studied strain demonstrated their promising application as nutrient food supplements and feed for animals.

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“…Thermophilic microalgae cultivated in large-scale open ponds are able to produce bulk amount of lipids, which can be utilised for biodiesel production technology [ 103 ]. Particularly, in recent years increasing attention were posed on the regulation of fatty acid dehydrogenase (FAD) in freeze resistant microalgae [ 59 , 104 , 105 ]. This studies contributed to both elucidation of the regulation of algal membrane assembly, and fatty acid metabolism.…”

Section: Extremophilic Microalgae As Bioreactorsmentioning

confidence: 99%

Advanced Applications for Protein and Compounds from Microalgae

Castiglia¹,

Landi

Esposito

2021

Plants

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Algal species still show unrevealed and unexplored potentiality for the identification of new compounds. Photosynthetic organisms represent a valuable resource to exploit and sustain the urgent need of sustainable and green technologies. Particularly, unconventional organisms from extreme environments could hide properties to be employed in a wide range of biotechnology applications, due to their peculiar alleles, proteins, and molecules. In this review we report a detailed dissection about the latest and advanced applications of protein derived from algae. Furthermore, the innovative use of modified algae as bio-reactors to generate proteins or bioactive compounds was discussed. The latest progress about pharmaceutical applications, including the possibility to obtain drugs to counteract virus (as SARS-CoV-2) were also examined. The last paragraph will survey recent cases of the utilization of extremophiles as bio-factories for specific protein and molecule production.

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Gene silencing of stearoyl-ACP desaturase enhances the stearic acid content in Chlamydomonas reinhardtii

Cited by 19 publications

References 46 publications

Time-dependent transcriptome profile of genes involved in triacylglycerol (TAG) and polyunsaturated fatty acid synthesis in Nannochloropsis gaditana during nitrogen starvation

Time-dependent transcriptome profile of genes involved in triacylglycerol (TAG) and polyunsaturated fatty acid synthesis in Nannochloropsis gaditana during nitrogen starvation

Effect of tobacco smoke on the growth, biochemical composition, and biodiesel characteristics of two Chlamydomonas strains

Advanced Applications for Protein and Compounds from Microalgae

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