Gene Silencing in Mammalian Cells with Light‐Activated Antisense Agents

Young, Douglas D.; Lusic, Hrvoje; Lively, Mark O.; Yoder, Jeffrey A.; Deiters, Alexander

doi:10.1002/cbic.200800627

Cited by 95 publications

(108 citation statements)

References 62 publications

(23 reference statements)

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“…www.chembiochem.org ture. [91] A second approach used ncPNA ASOs targeted at chordin and bozozok mRNA within zebrafish embryos. The ncPNA antisense strand was bound to a 2'-OMe sense strand (via a PL) which were introduced into zebrafish embryos (Figure 3).…”

Section: Gene Function Analysismentioning

confidence: 99%

Chemical Modification of Oligonucleotides for Therapeutic, Bioanalytical and other Applications

2009

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Although known for more than a century, bromine trifluoride is not a common reagent in day to day research work in organic chemistry. Its tendency to react exothermically with solvents containing Lewis base elements such as water, acetone or ethers distanced it from the mind of many. Still, under the proper conditions, it can perform quite a few selective reactions which are difficult to achieve by other reagents. We discuss in this review reactions which have been published in the last 30 years. They consist of fluorinating heteroatoms, substituting carbon‐halogen bonds with carbon‐fluorine bonds, syntheses of anesthetics, construction of the trifluoromethyl (CF3) and the difluoromethylene (CF2) groups, aromatic brominations and more.

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Section: Gene Function Analysismentioning

confidence: 99%

Chemical Modification of Oligonucleotides for Therapeutic, Bioanalytical and other Applications

2009

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“…[35] Recently the Deiters group also used an antisense approach in mammalian cells. [36] A full account of competing and complementary research is beyond the scope of this article but we are currently preparing a book chapter in which there will be much more room for these discussions. 6.…”

Section: Resultsmentioning

confidence: 97%

Light-Activatable Nucleic Acids 'Caged' at the Nucleobases

Buff¹,

Mack²,

Heckel³

2009

Chimia

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The attachment of photolabile groups to biologically active molecules offers a very versatile way to put a biological effect under the control of an external trigger signal and thus confers spatiotemporal and dose control to this effect. Over the last years we have prepared a number of oligonucleotide derivatives that are modified in such a way. These derivatives are usually referred to as 'caged compounds' and in particular our photolabile 'caging groups' are located at the nucleobases of oligonucleotides so that the Watson-Crick interaction is temporarily impossible. Thus several nucleic acid-based applications have now become controllable with light.

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“…When present in an oligonucleotide (42) causes a steric block to polymerase extension, but irradiation at 365nm results in unmasking of the thymine base and hence PCR is allowed to proceed. 184,185 The same protecting group has also been attached to O4 of thymidine, with similar photoactivated control of PCR. 186 Oligonucleotides bearing N3-azidoethyl and a N3-propargyl modifications at the termini have been used to prepare cyclic DNA via a triazole linkage.…”

Section: Oligonucleotides Containing Modified Sugarsmentioning

confidence: 99%

Nucleotides and Nucleic Acids; Oligo- and Polynucleotides

Loakes

2010

Organophosphorus Chemistry

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Gene Silencing in Mammalian Cells with Light‐Activated Antisense Agents

Cited by 95 publications

References 62 publications

Chemical Modification of Oligonucleotides for Therapeutic, Bioanalytical and other Applications

Chemical Modification of Oligonucleotides for Therapeutic, Bioanalytical and other Applications

Light-Activatable Nucleic Acids 'Caged' at the Nucleobases

Nucleotides and Nucleic Acids; Oligo- and Polynucleotides

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