Gene set enrichment analysis highlights different gene expression profiles in whole blood samples X-irradiated with low and high doses

El‐Saghire, Houssein; Thierens, Hubert; Monsieurs, Pieter; Michaux, Arlette; Vandevoorde, Charlot; Baatout, Sarah

doi:10.3109/09553002.2013.782448

Cited by 60 publications

(48 citation statements)

References 65 publications

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“…Previously, we have reported that MAPK cascades play a central role in whole blood samples irradiated with 0.05 Gy (5). In addition to that, studies on exposure to low doses have reported a proliferative response through the activation of ERK 1/2, p38 and JNK (34,35).…”

Section: Discussionmentioning

confidence: 87%

“…Previously, whole genome expression studies showed that high doses of ionizing radiation predominantly induced DNA damage and apoptosis with p53 signaling playing a key role, while low doses induced mainly cytokine and chemokine signaling (5). To clarify the mechanisms behind this immune response induction, we investigated the activation of central immune pathways in the primary human monocytes following exposure to low and high doses of ionizing radiation.…”

Section: Discussionmentioning

confidence: 99%

“…PF4 gene was upregulated in whole blood samples exposed to low doses of ionizing radiation (5). PF4 encodes for the platelet factor-4 protein, a chemokine expressed in platelets, T cells, monocytes, endothelial and muscle cells (32).…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we demonstrated that low doses of ionizing radiation induce immune responses in whole blood samples, with the gene set enrichment analysis showing the involvement of both innate and adaptive immunity (5). Monocytes are key players in the immune response.…”

Section: Introductionmentioning

confidence: 94%

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Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes

El‐Saghire

Michaux

Thierens

et al. 2013

International Journal of Molecular Medicine

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Abstract. The health effects arising from exposure to low doses of ionizing radiation are of particular concern, mainly due to the increased application of diagnostic and therapeutic X-ray modalities. The mechanisms behind the cell and tissue responses to low doses remain to be elucidated. Accumulating evidence suggests that low doses of ionizing radiation induce activation of the immune response; however, the processes involved have yet to be adequately investigated. Monocytes are key players in the induction of an immune response. Within the context of this study, we investigated the activation of toll-like receptors (TLRs), mitogen-activated protein kinases (MAPKs) and NF-κB signaling in isolated human primary monocytes in response to low doses (0.05 and 0

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

See 2 more Smart Citations

Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes

El‐Saghire

Michaux

Thierens

et al. 2013

International Journal of Molecular Medicine

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“…To overcome this problem, analysis tools have been developed, such as single gene statistical analysis methods (SGA), which are widely used to determine the differentially expressed genes, and the gene set enrichment analysis (GSEA), which aims to identify gene expression differences in groups of genes, for instance in those acting synergistically in a cell process (9,10). The two analytical methods were used concomitantly in this study.…”

Section: Introductionmentioning

confidence: 99%

Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts

Beck

Moreels

Quintens

et al. 2014

International Journal of Molecular Medicine

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Abstract. microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model was utilized to monitor gene expression changes in fetal skin fibroblasts of murine origin. Cells were continuously subjected for 65 h to a low dose (55 mSv) of ionizing radiation (IR), comprising a mixture of high-linear energy transfer (LET) neutrons and low-LET gamma-rays, and/or simulated microgravity using the random positioning machine (RPM), after which microarrays were performed. The data were analyzed both by gene set enrichment analysis (GSEA) and single gene analysis (SGA). Simulated microgravity affected fetal murine fibroblasts by inducing oxidative stress responsive genes. Three of these genes are targets of the nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), which may play a role in the cell response to simulated microgravity. In addition, simulated gravity decreased the expression of genes involved in cytoskeleton remodeling, which may have been caused by the downregulation of the serum response factor (SRF), possibly through the Rho signaling pathway. Similarly, chronic exposure to low-dose IR caused the downregulation of genes involved in cytoskeleton remodeling, as well as in cell cycle regulation and DNA damage response pathways. Many of the genes or gene sets that were altered in the individual treatments (RPM or IR) were not altered in the combined treatment (RPM and IR), indicating a complex interaction between RPM and IR.

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Individual Radiation Sensitivity and Biomarkers: Molecular Radiation Biology

Ainsbury,

Abrantes,

Baatout

et al. 2023

Radiobiology Textbook

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In recent years, scientific understanding of the changes radiation makes to the various tissues of the body has vastly increased. Identification of biological markers of radiation exposure and response has become a wide field with an increasing interest across the radiation research community. This chapter introduces the concepts of individual radiosensitivity, radiosusceptibility, and radiodegeneration, which are the key factors to classify radiation responses. Biomarkers are then introduced, and their key characteristics as well as classification are explained, with a particular focus on those biomarkers which have been identified for use in epidemiological studies of radiation risk—as this is a crucial topic of current interest within radiation protection. Brief information on collection of samples is followed by a detailed presentation of predictive assays in use in different settings including clinical applications with responses assessed chiefly in tissue biopsy or blood samples. The sections toward the end of this chapter then discuss the evidence associated with the relationship between age and separately sex, and radiosensitivity, as well as some genetic syndromes associated with radiosensitivity. The final section of this chapter provides a brief summary of how our current knowledge can further support individual, personalized, uses of radiation, particularly in clinical settings.

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Gene set enrichment analysis highlights different gene expression profiles in whole blood samples X-irradiated with low and high doses

Cited by 60 publications

References 65 publications

Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes

Low doses of ionizing radiation induce immune-stimulatory responses in isolated human primary monocytes

Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts

Individual Radiation Sensitivity and Biomarkers: Molecular Radiation Biology

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