Gene sensor using ferrocenyl oligonucleotide

Ihara, Toshihiro; Nakayama, Masamichi; Murata, Mitsuru; Nakano, Koji; Maeda, Mizuo

doi:10.1039/a703401f

Cited by 189 publications

(136 citation statements)

References 14 publications

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“…Another method relies on an end-labeled ODN that is complementary to the target (reporter probe), which is bound so that the label is next to the hybridized probe (Figure 4a) on the 5´ terminus (25). I In nd di ic ca at to or r--f fr re ee e s sy ys st te em ms s. .…”

Section: Dn Na a C Co On Nd Du Uc Ct Ti Iv Vi It Ty Ymentioning

confidence: 99%

Peer Reviewed: Detecting DNA Hybridization and Damage

E¹,

Fojta²

2001

Anal. Chem.

424

308

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Section: Dn Na a C Co On Nd Du Uc Ct Ti Iv Vi It Ty Ymentioning

confidence: 99%

Peer Reviewed: Detecting DNA Hybridization and Damage

E¹,

Fojta²

2001

Anal. Chem.

424

308

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“…A variety of approaches have been proposed and tested for electrochemical detection: (a) detecting the oxidative or reductive current of DNA bases directly [3,4]; (b) detecting the anodic current of DNA bases with the aid of a mediator [5][6][7]; (c) detecting the oxidation of DNA sugar moieties [8]; (d) detecting the electrochemical reaction of a non-covalent DNA binder [9][10][11][12][13][14][15][16][17]; (e) detecting the current generated from a covalently linked label inorganic and organic molecules, enzymes, and nanoparticles [18][19][20][21][22][23]. The advantages of electrochemical devices are their low-cost, fast response, simple design, small dimension and low power requirement.…”

Section: Introductionmentioning

confidence: 99%

“…Because many of the DNA binders are redoxactive, they have also been employed as electrochemical probes in the detection of DNA. Bard and colleagues [32,33] pioneered the field of electrochemical study of metal complex/DNA interaction, whereas Mikkelsen and others [9][10][11][12][13][14][15][16][17] used the redox-indicators in the detection of DNA hybridization events on electrode surfaces. The most promising indicators are the metal complexes containing the dipyrido[3,2-a :2 0 ,3 0 -c]phenazine (dppz) ligand, which have been found to intercalate into DNA with high affinity (K = 10 6 -10 7 M À1 ) due to its extended aromatic structure [29,34].…”

Section: Introductionmentioning

confidence: 99%

A new chemically amplified electrochemical system for the detection of biological affinity reactions: direct and competitive biotin assay

Guo

Yang²

2005

Analyst

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A newly developed chemically amplified electrochemical detection system was applied to the quantitation of DNA in solution. The system employed Ru(bpy) 2 dppz (bpy = 2,2 0 -bipyridine, dppz = dipyrido[3,2-a :2 0 ,3 0 -c]phenazine), a high-affinity DNA intercalator, as the electrochemical indicator, oxalate as the sacrificial electron donor to chemically amplify the electrochemical signal, and tin-doped indium oxide as the working electrode to suppress background current. Intercalation of Ru(bpy) 2 dppz into calf thymus DNA in solution led to a reduction in the oxalate-amplified electrochemical current as compared to a DNA-free solution. The degree of reduction was a function of the concentration of DNA, thus forming the basis for DNA detection. To illustrate the advantages of the new system, a direct comparison was made between amplified (with oxalate) and non-amplified (without oxalate) DNA detection. In the presence of 100 mM oxalate, anodic current of Ru(bpy) 2 dppz was amplified by more than 60 folds, resulting in substantial improvement in signal-to-background ratio. Furthermore, as the DNA concentration was increased, the amplified current decayed much faster than the non-amplified signal, giving rise to higher detection sensitivity. The steeper decay was attributed to slower redox reaction between DNA-intercalated Ru(bpy) 2 dppz and oxalate, as the negatively charged phosphate groups on DNA repelled the anions. Effect of ionic strength was investigated to provide support for the interpretation. As expected, the decay of the amplified response with increasing concentration of DNA became less steep when more NaCl was added into the solution. The opposite effect was observed when tri-propylamine, a cationic electron donor, was used instead of oxalate. With an optimized concentration of 30 mM oxalate and 5 lM Ru(bpy) 2 dppz, calf thymus DNA of as low as 1 pM was detected in solution, which was close to the detection limit of some fluorescence measurements.

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“…Synthesis of the ferrocene-modified dodecamer deoxyribothymine (FcT12) was made by the covalent coupling of N-(ferrocenecarbonyloxy)succinimide with the 5′-amino-modified deoxyribothymine dodecamer. 24,26 Similarly, the complementary sequence of K-ras was modified with ferrocene and was used as the model-target DNA (t12Fc). Each DNA sequence was dissolved in 50 mM phosphate buffer (pH 7) containing 100 mM NaCl to give a solution with a concentration of 100 μM (1 M = 1 mol dm -3 ).…”

Section: Biogenic Substances and Chemical Reagentsmentioning

confidence: 99%

“…23 Although this type of modification was originally developed for antisense oligonucleotide technologies, 24 we found that the particular derivative spontaneously forms self-assemblies on gold surfaces through chemisorption. 25 The technique represents DNA-backbone modification, and therefore allows us to arbitrarily assign the number as well as the location of the phosphorothioate function in the corresponding DNA sequence.…”

Section: Introductionmentioning

confidence: 99%

A Pivot-Hinge-Style DNA Immobilization Method with Adaptable Surface Concentration Based on Oligodeoxynucleotide-Phosphorothioate Chemisorption on Gold Surfaces

et al. 2012

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The chemisorption of oligodeoxynucleotide phosphorothioate (s-oligo) is reported. A series of s-oligo DNAs was designed for use as capture probe DNA molecules. The s-oligo DNAs consist of the K-ras gene (5′-GGA GCT GGT GGC-3′) and a dodecamer deoxyriboadenosine, both of which lie on either side of an s-oligo DNA sequence. By primarily focusing on the capture probe DNA having five-successive s-oligo sequences, e37, the immobilization chemistry of e37 was examined; atomic force microscopy achieved the direct visualization of individual molecules on Au (111) substrates, while a series of surface analyses, including IR, ellipsometry, and microgravimetry, showed that the s-oligo functional groups played a pivotal role in the surface-adlayer through the gold-thiol interaction. Interestingly, the amount of immobilization showed a definite relationship with the number of s-oligo linkages introduced, which should be important to regulate the concentration of the capture probe DNA molecules on the surface. Some preliminary studies using ferrocene-modified complementary sequences indicated that electrochemical labeling and readouts were possible.

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Gene sensor using ferrocenyl oligonucleotide

Cited by 189 publications

References 14 publications

Peer Reviewed: Detecting DNA Hybridization and Damage

Peer Reviewed: Detecting DNA Hybridization and Damage

A new chemically amplified electrochemical system for the detection of biological affinity reactions: direct and competitive biotin assay

A Pivot-Hinge-Style DNA Immobilization Method with Adaptable Surface Concentration Based on Oligodeoxynucleotide-Phosphorothioate Chemisorption on Gold Surfaces

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