Gene regulation associated with sexual development and female fertility in different isolates of Trichoderma reesei

Dattenböck, Christoph; Tisch, Doris; Schuster, André; Monroy, Alberto Alonso; Hinterdobler, Wolfgang; Schmoll, Monika

doi:10.1186/s40694-018-0055-4

Cited by 15 publications

(16 citation statements)

References 60 publications

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“…For T. reesei of those nutrients only glucose was shown so far to be sensed by GPCRs 64 . Additionally, we compared expression patterns under conditions of sexual development and growth on cellulose 65,66 .…”

Section: Resultsmentioning

confidence: 99%

Protein phosphatases regulate growth, development, cellulases and secondary metabolism in Trichoderma reesei

Rodriguez-Iglesias

Schmoll

2019

Sci Rep

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Trichoderma reesei represents one of the most prolific producers of plant cell wall degrading enzymes. Recent research showed broad regulation by phosphorylation in T. reesei , including important transcription factors involved in cellulase regulation. To evaluate factors crucial for changes in these phosphorylation events, we studied non-essential protein phosphatases (PPs) of T. reesei . Viable deletion strains were tested for growth on different carbon sources, osmotic and oxidative stress response, asexual and sexual development, cellulase and protease production as well as secondary metabolism. Six PPs were found to be positive or negative regulators for cellulase production. A correlation of the effects of PPs on protease activities and cellulase activities was not detected. Hierarchical clustering of regulation patterns and phenotypes of deletion indicated functional specialization within PP classes and common as well as variable effects. Our results confirmed the central role of catalytic and regulatory subunits of PP2A which regulates several aspects of cell growth and metabolism. Moreover we show that the additional homologue of PPH5 in Trichoderma spp., PPH5-2 assumes distinct functions in metabolism, development and stress response, different from PPH5. The influence of PPs on both cellulase gene expression and secondary metabolite production support an interrelationship in the underlying regulation mechanisms.

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Section: Resultsmentioning

confidence: 99%

Protein phosphatases regulate growth, development, cellulases and secondary metabolism in Trichoderma reesei

Rodriguez-Iglesias

Schmoll

2019

Sci Rep

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“…3 https://mycocosm.jgi.doe.gov/Trire2/Trire2.home.html is differentially regulated between different carbon sources (Stappler et al, 2017a). Comparative analysis showed upregulation of gpr8 under conditions of sexual development compared to growth on cellulose (>2-fold; p-value <0.005) in different T. reesei strains (Dattenböck et al, 2018). In order to gain first information on the function of GPR8 in T. reesei, we checked co-regulated genes under different conditions in publicly available datasets (Supplementary Material, Table 1).…”

Section: Gpr8 Is Regulated By Light and Associated With Metabolismmentioning

confidence: 99%

“…Although not specifically enriched, co-regulated genes in both conditions comprised numerous genes involved in secondary metabolism. Coregulation under conditions related to development (Dattenböck et al, 2018) revealed significant enrichment of genes associated with metabolism (p-value 7.8 E-04) and in particular in secondary metabolism (p-value 2.4 E-03) (Supplementary Material, Table 1). Consequently, investigation of functions of GPR8 in enzyme production and secondary metabolism should reveal the physiological role of this receptor.…”

Section: Gpr8 Is Regulated By Light and Associated With Metabolismmentioning

confidence: 99%

The G-protein Coupled Receptor GPR8 Regulates Secondary Metabolism in Trichoderma reesei

Hinterdobler

Beier

Monroy

et al. 2020

Front. Bioeng. Biotechnol.

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Changing environmental conditions are of utmost importance for regulation of secondary metabolism in fungi. Different environmental cues including the carbon source, light and the presence of a mating partner can lead to altered production of compounds. Thereby, the heterotrimeric G-protein pathway is of major importance for sensing and adjustment of gene regulation. Regulation of secondary metabolism is crucial in the biotechnological workhorse Trichoderma reesei for knowledge-based adjustment in industrial fermentations, but also with respect to the potential use as a host for heterologous compound production. We investigated the function of the class VII G-protein coupled receptor (GPCR) gene gpr8 that is localized in the vicinity of the SOR cluster, which is responsible for biosynthesis of sorbicillinoids. GPR8 positively impacts regulation of the genes in this cluster in darkness. Accordingly, abundance of trichodimerol and dihydrotrichotetronine as well as other secondary metabolites is decreased in the deletion mutant. Transcriptome analysis moreover showed the major role of GPR8 being exerted in darkness with a considerable influence on regulation of secondary metabolism. Genes regulated in gpr8 overlap with those regulated directly or indirectly by the transcription factor YPR2, especially concerning genes related to secondary metabolism. The predicted FAD/FMN containing dehydrogenase gene sor7, one of the positive targets of the cascade triggered by GPR8, has a positive effect on secondary metabolite production, but also cellulase gene expression. Hence SOR7 has some overlapping, but also additional functions compared to GPR8. The G-protein coupled receptor GPR8 exerts a light dependent impact on secondary metabolism, which is in part mediated by the transcription factor YPR2 and the function of SOR7. Hence, T. reesei may apply GPR8 to adjust production of secondary metabolites and hence chemical communication to signals from the environment.

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“…A 2019 collaborative review highlighted the present state of knowledge and raised a multitude of open questions regarding the diversity and function of fungi in marine ecosystems [13].The symbiont assemblages inside the sponge are well organized in biofilms or dense colonies and are stabilized in the skeleton network over time [14,15]. This certainly impacts their development steps and the expression of biosynthetic clusters of secondary metabolites because it is now well documented that, in fungi, secondary metabolism and life cycle among fungi are co-regulated at the genomic level [16][17][18].This idea drives us to compare the metabolic profile of fungi cultivated on a gar slants and in liquid state. The result is that solid-state cultivation often leads to larger molecular diversity than classical liquid state fermentation LSF [19][20][21].…”

mentioning

confidence: 99%

“…The symbiont assemblages inside the sponge are well organized in biofilms or dense colonies and are stabilized in the skeleton network over time [14,15]. This certainly impacts their development steps and the expression of biosynthetic clusters of secondary metabolites because it is now well documented that, in fungi, secondary metabolism and life cycle among fungi are co-regulated at the genomic level [16][17][18].…”

mentioning

confidence: 99%

Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa

et al. 2019

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The fungi Chrysosporium lobatum TM-237-S5 was isolated from the sponge Acanthella cavernosa, collected from the mesophotic coral ecosystem of the Red Sea. The strain was cultivated on a potato dextrose agar (PDA) medium, coupling solid-state fermentation and solid-state extraction (SSF/SSE) with a neutral macroreticular polymeric adsorbent XAD Amberlite resin (AMBERLITE XAD1600N). The SSF/SSE lead to high chemodiversity and productivity compared to classical submerged cultivation. Ten phenalenone related compounds were isolated and fully characterized by one-dimensional and two-dimensional NMR and HRMS. Among them, four were found to be new compounds corresponding to isoconiolactone, (-)-peniciphenalenin F, (+)-8-hydroxyscleroderodin, and (+)-8-hydroxysclerodin. It is concluded that SSF/SSE is a powerful strategy, opening a new era for the exploitation of microbial secondary metabolites.the massive presence of microorganisms in the mesophyl matrix of the sponges, representing around 50% of their biomass [9][10][11].Fungi in the marine environment, and especially those associated with marine invertebrates, have been extensively investigated and reviewed [12,13]. A 2019 collaborative review highlighted the present state of knowledge and raised a multitude of open questions regarding the diversity and function of fungi in marine ecosystems [13].The symbiont assemblages inside the sponge are well organized in biofilms or dense colonies and are stabilized in the skeleton network over time [14,15]. This certainly impacts their development steps and the expression of biosynthetic clusters of secondary metabolites because it is now well documented that, in fungi, secondary metabolism and life cycle among fungi are co-regulated at the genomic level [16][17][18].This idea drives us to compare the metabolic profile of fungi cultivated on a gar slants and in liquid state. The result is that solid-state cultivation often leads to larger molecular diversity than classical liquid state fermentation LSF [19][20][21]. The major obstacle that stands against agar cultivation is the scale-up. In order to overcome such a challenge, we have developed specific innovative technologies, namely Platotex [22,23] and, more recently, Unifertex [24]. As we systematically coupled the culture of microorganisms with in-situ solid phase extraction (SPE), we also developed a specific SPE procedure for agar cultivation, termed solid-solid extraction (SSE) [25].In the present study, we report the impact of agar-supported cultivation on the production of secondary metabolites by the marine fungi Chrysosporium lobatum TM-237-S5, isolated from the Red Sea sponge Acanthella cavernosa. Chrysosporium lobatum was previously reported in the literature as a mosquito pathogenic fungus [26]. However, a very limited number of secondary metabolites have been reported in the literature for the genus Chrysosporium. Thus, the strain Chrysosporium queenslandicum IFM produced naphthaquinone-type altersolanols A, B, and C, the antifungal queenslandon, a representative...

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Gene regulation associated with sexual development and female fertility in different isolates of Trichoderma reesei

Cited by 15 publications

References 60 publications

Protein phosphatases regulate growth, development, cellulases and secondary metabolism in Trichoderma reesei

Protein phosphatases regulate growth, development, cellulases and secondary metabolism in Trichoderma reesei

The G-protein Coupled Receptor GPR8 Regulates Secondary Metabolism in Trichoderma reesei

Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa

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