Gene Regulation and Cellular Metabolism: An Essential Partnership

Carthew, Richard W.

doi:10.1016/j.tig.2020.09.018

Cited by 40 publications

(29 citation statements)

References 64 publications

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“…Originally, the major roles of metabolism were considered to be the conversion of food into energy and building blocks for macromolecules and the elimination of metabolic waste. However, more recently, it has become clear that cellular metabolism is tightly connected to multiple cellular processes and the orchestration of the expression of gene cassettes that control these critical processes, including quiescence and activation of stem cells, tissue homeostasis and repair after injury, immune and inflammatory responses, tumor immunosurveillance, cell migration, development and lineage commitment and senescence [ 1 , 2 , 3 , 16 ]. Metabolic pathways provide the molecules necessary for gene regulation, e.g., NAD + , SAM-e and Acetyl-CoA [ 1 ] as well as ATP, the primary fuel driving gene expression.…”

Section: Discussionmentioning

confidence: 99%

“…However, more recently, it has become clear that cellular metabolism is tightly connected to multiple cellular processes and the orchestration of the expression of gene cassettes that control these critical processes, including quiescence and activation of stem cells, tissue homeostasis and repair after injury, immune and inflammatory responses, tumor immunosurveillance, cell migration, development and lineage commitment and senescence [ 1 , 2 , 3 , 16 ]. Metabolic pathways provide the molecules necessary for gene regulation, e.g., NAD + , SAM-e and Acetyl-CoA [ 1 ] as well as ATP, the primary fuel driving gene expression. However, cells must constantly adjust their metabolism based upon highly variable metabolite and energy budgets dependent on cellular status, i.e., quiescent versus activated, and nutrient and oxygen availability within their microenvironment.…”

Section: Discussionmentioning

confidence: 99%

“…However, cells must constantly adjust their metabolism based upon highly variable metabolite and energy budgets dependent on cellular status, i.e., quiescent versus activated, and nutrient and oxygen availability within their microenvironment. The molecular mechanisms that connect metabolic function with the regulation of gene expression in cells to control critical cellular states, including quiescence, activation, proliferation, migration and differentiation, are just beginning to come into focus [ 1 ]. The integration of nutrient availability and metabolism and the coupling of energy production with cellular status is highly evolutionarily conserved.…”

Section: Discussionmentioning

confidence: 99%

“…The integration of a cell’s state with its metabolism is critically important and the coupling of energy production and cellular function is highly evolutionarily conserved. This has been demonstrated in stem cell biology, organismal, cellular and tissue differentiation and, perhaps most extensively, in immune cell biology [ 1 , 2 , 3 ]. However, a molecular understanding of how cells coordinate and couple metabolism with transcription as they navigate quiescence, growth, proliferation, differentiation and migration remains in its infancy.…”

Section: Introductionmentioning

confidence: 99%

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Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation

Ono²,

Chimge

et al. 2021

Cancers

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The integration of cellular status with metabolism is critically important and the coupling of energy production and cellular function is highly evolutionarily conserved. This has been demonstrated in stem cell biology, organismal, cellular and tissue differentiation and in immune cell biology. However, a molecular mechanism delineating how cells coordinate and couple metabolism with transcription as they navigate quiescence, growth, proliferation, differentiation and migration remains in its infancy. The extreme N-termini of the Kat3 coactivator family members, CBP and p300, by far the least homologous regions with only 66% identity, interact with members of the nuclear receptor family, interferon activated Stat1 and transcriptionally competent β-catenin, a critical component of the Wnt signaling pathway. We now wish to report based on multiomic and functional investigations, utilizing p300 knockdown, N-terminal p300 edited and p300 S89A edited cell lines and p300 S89A knockin mice, that the N-termini of the Kat3 coactivators provide a highly evolutionarily conserved hub to integrate multiple signaling cascades to coordinate cellular metabolism with the regulation of cellular status and function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation

Ono²,

Chimge

et al. 2021

Cancers

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show abstract

“…To investigate the intracellular metabolic process of propionic acid [28][29][30][31], the metabolic flux of key nodes was analyzed and used to guide the regulation of fermentation process. Metabolic flux analysis of glucose-6-phosphate node: Glucose-6-phosphate was the first key metabolic node, participating in the pentose phosphate pathway and glycolysis pathway.…”

Section: Metabolic Flux Analysis Of Key Nodesmentioning

confidence: 99%

Efficient Production of Propionic Acid in the Fed-Batch Fermentation of Propionibacterium acidipropionici and Its Metabolic Flux Analysis

Zhang¹,

Zhang²,

X³

et al. 2021

austinjnutrmetab

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To improve the fermentation efficiency of Propionibacterium acidipropionici, a simplified metabolic network was established to provide theoretical guidance for medium optimization and process regulation. The effect of glucose and glycerol on propionic acid production and metabolic flux distribution was investigated and the combination of glucose and glycerol was optimized. The results showed that the productivity of propionic acid could be improved by enhancing the synthesis of pyruvate and its flux distribution to the oxaloacetate branch. Finally, the scaled-up fed-batch fermentation of P. acidipropionici was conducted. The concentration of propionic acid reached 51.75 ± 3.62g/L with a glucose/glycerol ratio of 4:1, an improvement of 79.25% relative to the use of glucose alone, and the corresponding productivity and yield were 0.39g/(L· h) and 0.52g/g, respectively. Therefore, the combination of glucose and glycerol significantly improved the productivity of propionic acid and provides a new strategy for industrial production.

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Towards Mapping Mouse Metabolic Tissue Atlas by Mid‐Infrared Imaging with Heavy Water Labeling

Liu

Shi

et al. 2022

Advanced Science

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Understanding metabolism is of great significance to decipher various physiological and pathogenic processes. While great progress has been made to profile gene expression, how to capture organ-, tissue-, and cell-type-specific metabolic profile (i.e., metabolic tissue atlas) in complex mammalian systems is lagging behind, largely owing to the lack of metabolic imaging tools with high resolution and high throughput. Here, the authors applied mid-infrared imaging coupled with heavy water (D 2 O) metabolic labeling to a scope of mouse organs and tissues. The premise is that, as D 2 O participates in the biosynthesis of various macromolecules, the resulting broad C-D vibrational spectrum should interrogate a wide range of metabolic pathways. Applying multivariate analysis to the C-D spectrum, the authors successfully identified both inter-organ and intra-tissue metabolic signatures of mice. A large-scale metabolic atlas map between different organs from the same mice is thus generated. Moreover, leveraging the power of unsupervised clustering methods, spatially-resolved metabolic signatures of brain tissues are discovered, revealing tissue and cell-type specific metabolic profile in situ. As a demonstration of this technique, the authors captured metabolic changes during brain development and characterized intratumoral metabolic heterogeneity of glioblastoma. Altogether, the integrated platform paves a way to map the metabolic tissue atlas for complex mammalian systems.

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Gene Regulation and Cellular Metabolism: An Essential Partnership

Cited by 40 publications

References 64 publications

Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation

Differential Kat3 Usage Orchestrates the Integration of Cellular Metabolism with Differentiation

Efficient Production of Propionic Acid in the Fed-Batch Fermentation of Propionibacterium acidipropionici and Its Metabolic Flux Analysis

Towards Mapping Mouse Metabolic Tissue Atlas by Mid‐Infrared Imaging with Heavy Water Labeling

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