Gene Recombination in Escherichia Coli

Lederberg, Joshua; Tatum, E. L.

doi:10.1038/158558a0

Cited by 540 publications

(289 citation statements)

References 2 publications

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“…Long after the Wrst discovery of bacterial conjugation, which dates back to 1946 (Lederberg and Tatum, 1946), a series of pathogenicity-associated secretion systems delivering toxic protein or DNA substrates into eukaryotic host cells were discovered to be sequence-related to bacterial conjugation systems (Lessl and Lanka, 1994;Lessl et al, 1992). These secretion systems are grouped into the family of type IV secretion systems (T4SS), as originally proposed by Salmond (1994) (Table 1).…”

Section: Introductionmentioning

confidence: 99%

The mating pair formation system of conjugative plasmids—A versatile secretion machinery for transfer of proteins and DNA

Schröder

Lanka

2005

Plasmid

188

146

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The mating pair formation (Mpf) system functions as a secretion machinery for intercellular DNA transfer during bacterial conjugation. The components of the Mpf system, comprising a minimal set of 10 conserved proteins, form a membrane-spanning protein complex and a surface-exposed sex pilus, which both serve to establish intimate physical contacts with a recipient bacterium. To function as a DNA secretion apparatus the Mpf complex additionally requires the coupling protein (CP). The CP interacts with the DNA substrate and couples it to the secretion pore formed by the Mpf system. Mpf/CP conjugation systems belong to the family of type IV secretion systems (T4SS), which also includes DNA-uptake and -release systems, as well as eVector protein translocation systems of bacterial pathogens such as Agrobacterium tumefaciens (VirB/VirD4) and Helicobacter pylori (Cag). The increased eVorts to unravel the molecular mechanisms of type IV secretion have largely advanced our current understanding of the Mpf/CP system of bacterial conjugation systems. It has become apparent that proteins coupled to DNA rather than DNA itself are the actively transported substrates during bacterial conjugation. We here present a uniWed and updated view of the functioning and the molecular architecture of the Mpf/CP machinery. 

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Section: Introductionmentioning

confidence: 99%

The mating pair formation system of conjugative plasmids—A versatile secretion machinery for transfer of proteins and DNA

Schröder

Lanka

2005

Plasmid

188

146

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“…Structural changes might be detected in "sexual" species by their effect on crossing-over, but no aberrations were detected in 128 X-ray and mustard treated chromosomes of Escherichia coli thus tested (Lederberg, 1947b). Whether complex genetic alterations, described by Luria (1946) as occurring too frequently to be accounted for coincidence, are chromosomal aberrations remains to be determined.…”

Section: Modes Of Genetic Variation (I) the Particulate Basismentioning

confidence: 93%

“…Gene recombination has been discovered in a strain of Escheric/zia coli, permitting for the first time of a mendelian or combinatorial analysis of bacterial mutations (Lederberg, 1947b;Lederberg and Tatum, 1946a i946b; Tatum and Lederberg, 1947). Gene recombination was inferred from the presence in mixed populations of multiple biochemical mutants of types characterisable as recombinations of the genotypes of the "parents."…”

Section: Gene Recombination In Bacteria and Bacteriophagementioning

confidence: 99%

Problems in microbial genetics

Lederberg

1948

Heredity

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“…The following linear order, as after Lederberg (1947) with minor additions was found to be a satisfactory guide to interpret the results of the crosses:…”

Section: Methodsmentioning

confidence: 99%

“…colonies growing on the minimal medium, were scored for segregating markers according to the methods given by Lederberg (1947). In most crosses however, in order to avoid parental contamination, which might have been of nuisance for resistance testing, each prototrophic colony was plated on complete agar, one colony picked at random and tested for prototrophism while scored for markers.…”

Section: Methodsmentioning

confidence: 99%