Gene Pyramiding Improved Resistance to Angular Leaf Spot in Common Bean

Ddamulira, G.; Mukankusi, Clare Mugisha; Ochwo-Ssemakula, Mildred; Edema, Richard; Sseruwagi, Peter; Gepts, Paul

doi:10.9734/ajea/2015/17833

Cited by 14 publications

(14 citation statements)

References 28 publications

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“…Given the high diversity of P. griseola races found in Africa (Wagara et al, 2011; Ddamulira et al, 2014b; Leitich, 2016; Kijana et al, 2017), more than one resistance gene will be required to confer durable ALS resistance to a wide range of races. Ddamulira et al (2015) pyramided resistance genes from AND 277 ( Phg‐1 ), Mexico 54 ( Phg‐2 ), and G5686 ( Phg‐4 , Phg‐5 , and Phg G5686C ) and introgressed the resistance into the susceptible cultivars Kanyebwa (local landrace) and CAL 96. The presence of the resistance loci was verified by molecular markers, and the resulting population showed reduced ALS symptom severity compared with single crosses when inoculated with race 61‐63 (Ddamulira et al, 2015).…”

Section: Breeding For Angular Leaf Spot Resistance In East Africamentioning

confidence: 99%

“…Research on ALS in East Africa has focused on the identification and genetic characterization of new sources of resistance in local landraces and released varieties (Ng'ayu-Wanjau, 2013;Ddamulira et al, 2014a;Leitich, 2016;Kijana et al, 2017). In these studies, Mexico 54 was found to be resistant to most races of P. griseola, and this cultivar is the most common source of ALS resistance in breeding in Uganda and East Africa (Namayanja et al, 2006;Chilagane et al, 2013;Ng'ayu-Wanjau, 2013;Ddamulira et al, 2015;Miller et al, 2018).…”

Section: Breeding For Angular Leaf Spot Resistance In East Africamentioning

confidence: 99%

“…Resistance loci are stated with their new name accepted by the Bean Improvement Cooperative Genetics Committee and their originally published name. P. griseola, though this cultivar was resistant to only 5 of 19 Colombian races used in one study (CIAT, 2005;Chilagane et al, 2013;Ng'ayu-Wanjau, 2013;Ddamulira et al, 2015). The putative Phg-2 allele of G10474 confers broad resistance to most races of P. griseola, and it has been extensively used in breeding at CIAT.…”

Section: Allelesmentioning

confidence: 99%

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A Review of Angular Leaf Spot Resistance in Common Bean

et al. 2019

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Angular leaf spot (ALS), caused by Pseudocercospora griseola, is one of the most devastating diseases of common bean (Phaseolus vulgaris L.) in tropical and subtropical production areas. Breeding for ALS resistance is difficult due to the extensive virulence diversity of P. griseola and the recurrent appearance of new virulent races. Five major loci, Phg‐1 to Phg‐5, conferring ALS resistance have been named, and markers tightly linked to these loci have been reported. Quantitative trait loci (QTLs) have also been described, but the validation of some QTLs is still pending. The Phg‐1, Phg‐4, and Phg‐5 loci are from common bean cultivars of the Andean gene pool, whereas Phg‐2 and Phg‐3 are from beans of the Mesoamerican gene pool. The reference genome of common bean and high‐throughput sequencing technologies are enabling the development of molecular markers closely linked to the Phg loci, more accurate mapping of the resistance loci, and the comparison of their genomic positions. The objective of this report is to provide a comprehensive review of ALS resistance in common bean. Furthermore, we are reporting three case studies of ALS resistance breeding in Latin America and Africa. This review will serve as a reference for future resistance mapping studies and as a guide for the selection of resistance loci in breeding programs aiming to develop common bean cultivars with durable ALS resistance.

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Section: Breeding For Angular Leaf Spot Resistance In East Africamentioning

confidence: 99%

Section: Breeding For Angular Leaf Spot Resistance In East Africamentioning

confidence: 99%

Section: Allelesmentioning

confidence: 99%

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A Review of Angular Leaf Spot Resistance in Common Bean

et al. 2019

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“…Ferreira et al (2012) used SCAR, CAPS, and RAPD markers to successfully pyramid Co-2, Co-3/9 anthracnose and I and bc-3 common mosaic virus resistance genes into the 'fabada' market class A25 genotype. Ddamulira et al (2015) reported the effectiveness of gene pyramiding in improving angular leaf spot resistance in susceptible common bean cultivar. Recently, reported marker-assisted pyramiding of bacterial blight and gall midge resistance genes (Gm4, Gm8, and Xa21) into 'RPHR-1005' the restorer line of the popular rice hybrid 'DRRH-3' and the variety developed with cumulating thee genes were better yield and increased disease resistance trait.…”

Section: Gene Pyramiding and Cultivar Developmentmentioning

confidence: 99%

“…Marker-assisted selection (MAS) and gene pyramiding have been reported in common bean research (Kelly et al, 2003, Miklas et al, 2006Ragagnin et al, 2009); Ferreira et al, 2012;. Recently, Ddamulira et al, (2015) reported the efficiency and effectiveness of gene pyramiding in improving angular leaf spot resistance in susceptible common bean cultivar.…”

Section: Introductionmentioning

confidence: 99%

Marker-assisted Pyramiding Resistance Genes Against Angular Leaf Spot and Common Bacterial Blight Disease into Preferred Common Bean Cultivar

Rezene¹,

Tesfaye

Mukankusi

et al. 2019

mpb

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Angular leaf spot (ALS) caused by Pseudocercospora griseola and common bacterial blight (CBB) caused by Xanthomonas campestris pv phaseoli X. campestris pv. phaseoli var. fuscans are the most economically important diseases of common bean production in Ethiopia. This research aims at pyramiding the Phg-2 R gene for angular leaf spot resistance and two CBB major resistance quantitative trait loci (RQTLs) into the background of the most popular and susceptible common bean cultivar "REDWOLAITA" (RW) with the aid of marker-assisted breeding method. Marker-assisted Parallel Back Crossing (MAPBC) breeding scheme with three separate parallel backcrossing streams were adopted for tracking three independent resistance loci linked to g796 (Phg-2 for ALS resistance) and, SU91 and SAP6 genetic markers from two different donor parents to the REDWOLAITA recurrent parent. The two donor parental lines VAX6 (with known RQTLs tagged by the SAP6 and SU91 genetic markers on linkage groups 10 and 8, respectively) and MEX54 with the Phg-2 R gene tagged by the g796 genetic marker at the linkage group 8 were used in the gene pyramiding program. After the BC4 generation, progenies that combined SAP6 and g796 genetic markers were created and selected from the BC4 inter-crossing of progenies. Then, further inter-crossing was made between selected progenies that combined the SAP6 and g796 genetic markers with selected progenies with the SU91 genetic marker. Finally, from this study we developed Monogenic Near Isogenic Lines (MNILs) with R genes tagged by the SAP6, g796, and SU91 molecular markers and polygenic PNILs with different gene combination includes MNIL SAP6 , MNIL SU91 & MNIL g796 , polygenetic PNILs SAP6/g796 , PNILs SU91/g796 , PNILs SAP6/SU , PNILs SAP6/g796/SAP6 , with more than 97% genome recovered from the RW genetic background. Marker-assisted backcrossing facilitated selection of progenies that combined good agronomic traits with resistance loci were constructed from the RW common bean cultivar genetic background and tested under the screening house condition. The developed lines showed high level of disease resistance to the strains of CBB and ALS present under the screening conditions. They were selected to be multiplied and tested under multiple environment, before varietal release and wider production. Developed MNILs with good agronomic background will also be used as alternative donor parent for the future gene pyramiding program.

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