Gene pyramiding combinations confer resistance of Asian soybean rust

Meira, Daniela; Panho, Maiara Cecília; Beche, Eduardo; Woyann, Leomar Guilherme; Madella, Laura Alexandra; Milioli, Anderson Simionato; Colonelli, Lucas Leite; Malone, Gaspar; Júnior, Salvador Brito; Benin, Giovani

doi:10.1002/csc2.20700

Cited by 6 publications

(3 citation statements)

References 74 publications

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“…Soybean lines carrying different combinations of Rpp2 , Rpp3 , Rpp4 and Rpp5 were developed and showed increased resistance to P. pachyrhizi isolates from Japan, Brazil (Yamanaka et al ., 2015 ) and Bangladesh (Yamanaka and Hossain, 2019 ). Rpp1‐b + Rpp5 was the most effective combination of Rpp genes conferring resistance to naturally occurring P. pachyrhizi isolates in soybean fields in Brazil (Meira et al ., 2022 ). Different combinations of Rpp1 , Rpp1‐b , Rpp2 , Rpp3 , Rpp4 and Rpp5 have also been evaluated under field conditions with naturally occurring SBR isolates in Parana state, Brazil, with lines harbouring Rpp2 + Rpp4 or Rpp2 + Rpp1‐b showing the most effective resistance (Panho et al ., 2022 ).…”

Section: Approaches For Improving Sbr Resistance I...mentioning

confidence: 99%

Soybean‐Phakopsora pachyrhizi interactions: towards the development of next‐generation disease‐resistant plants

Chicowski,

Bredow,

Utiyama

et al. 2023

Plant Biotechnology Journal

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SummarySoybean rust (SBR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is a devastating foliar disease threatening soybean production. To date, no commercial cultivars conferring durable resistance to SBR are available. The development of long‐lasting SBR resistance has been hindered by the lack of understanding of this complex pathosystem, encompassing challenges posed by intricate genetic structures in both the host and pathogen, leading to a gap in the knowledge of gene‐for‐gene interactions between soybean and P. pachyrhizi. In this review, we focus on recent advancements and emerging technologies that can be used to improve our understanding of the P. pachyrhizi‐soybean molecular interactions. We further explore approaches used to combat SBR, including conventional breeding, transgenic approaches and RNA interference, and how advances in our understanding of plant immune networks, the availability of new molecular tools, and the recent sequencing of the P. pachyrhizi genome could be used to aid in the development of better genetic resistance against SBR. Lastly, we discuss the research gaps of this pathosystem and how new technologies can be used to shed light on these questions and to develop durable next‐generation SBR‐resistant soybean plants.

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Section: Approaches For Improving Sbr Resistance I...mentioning

confidence: 99%

Soybean‐Phakopsora pachyrhizi interactions: towards the development of next‐generation disease‐resistant plants

Chicowski,

Bredow,

Utiyama

et al. 2023

Plant Biotechnology Journal

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“…Therefore, a combination of molecular marker technology and conventional cultivation techniques, in addition to the introduction of specific PCR marker technology, to select high-quality and high-yield offspring have been verified to accelerate cultivation [50]. To date, most studies of gene pyramids have focused on disease resistance and yield, while gene-pyramiding-related quality is still poor [1]. In this study, three wheat cultivars-Hengguan 35, Zhengmai 366 and Zhengmai 7698-were used to improve wheat quality by polymerizing three highquality beneficial genes through molecular-marker-assisted selection.…”

Section: Marker-assisted Selection Accelerating the Cultivation Of Hi...mentioning

confidence: 99%

“…Due to rising living standards, consumers and processing enterprises have increased the requirements for wheat quality and food safety. Gene pyramiding refers to the accumulation of beneficial target genes that are dispersed in different genetic resources in the same germplasm to cultivate unique wheat varieties with multiple desired traits [1]. The traditional phenotypic identification method cannot readily be used to efficiently select individual plants that aggregate several target genes.…”

Section: Introductionmentioning

confidence: 99%

Quality and Agronomic Trait Analyses of Pyramids Composed of Wheat Genes NGli-D2, Sec-1s and 1Dx5+1Dy10

Fang

et al. 2023

IJMS

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Due to rising living standards, it is important to improve wheat’s quality traits by adjusting its storage protein genes. The introduction or locus deletion of high molecular weight subunits could provide new options for improving wheat quality and food safety. In this study, digenic and trigenic wheat lines were identified, in which the 1Dx5+1Dy10 subunit, and NGli-D2 and Sec-1s genes were successfully polymerized to determine the role of gene pyramiding in wheat quality. In addition, the effects of ω-rye alkaloids during 1BL/1RS translocation on quality were eliminated by introducing and utilizing 1Dx5+1Dy10 subunits through gene pyramiding. Additionally, the content of alcohol-soluble proteins was reduced, the Glu/Gli ratio was increased and high-quality wheat lines were obtained. The sedimentation values and mixograph parameters of the gene pyramids under different genetic backgrounds were significantly increased. Among all the pyramids, the trigenic lines in Zhengmai 7698, which was the genetic background, had the highest sedimentation value. The mixograph parameters of the midline peak time (MPT), midline peak value (MPV), midline peak width (MPW), curve tail value (CTV), curve tail width (CTW), midline value at 8 min (MTxV), midline width at 8 min (MTxW) and midline integral at 8 min (MTxI) of the gene pyramids were markedly enhanced, especially in the trigenic lines. Therefore, the pyramiding processes of the 1Dx5+1Dy10, Sec-1S and NGli-D2 genes improved dough elasticity. The overall protein composition of the modified gene pyramids was better than that of the wild type. The Glu/Gli ratios of the type I digenic line and trigenic lines containing the NGli-D2 locus were higher than that of the type II digenic line without the NGli-D2 locus. The trigenic lines with Hengguan 35 as the genetic background had the highest Glu/Gli ratio among the specimens. The unextractable polymeric protein (UPP%) and Glu/Gli ratios of the type II digenic line and trigenic lines were significantly higher than those of the wild type. The UPP% of the type II digenic line was higher than that of the trigenic lines, while the Glu/Gli ratio was slightly lower than that of the trigenic lines. In addition, the celiac disease (CD) epitopes’ level of the gene pyramids significantly decreased. The strategy and information reported in this study could be very useful for improving wheat processing quality and reducing wheat CD epitopes.

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Rpp-Gene pyramiding confers higher resistance level to Asian soybean rust

et al. 2022

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Asian soybean rust (ASR) causes large reductions in soybean yield, affecting the entire grain market. With low fungicide e ciency, the use of resistant cultivars can be an economical, safe, e cient, and sustainable control alternative. However, the great variability and aggressiveness of ASR and the use of Rpp genes are limited. Thus, gene pyramiding is a promising strategy for the development of cultivars with high resistance to a greater number of isolates. Thus, the aim of this study was to evaluate sister lines with different pyramided Rpp gene for resistance to Phakopsora pachyrhizi and identify which combination of Rpp genes had higher levels of resistance under eld conditions. All Rpp-pyramided lines showed higher levels of resistance, with signi cant reductions in sporulation levels (SL), number of uredinia per lesion (NoU), and frequency of lesions with uredinia (%LU), compared to the resistance sources PI200487 (Rpp5), PI200492 (Rpp1), PI230970 (Rpp2), PI459025A (Rpp4), PI506764 (Rpp3, 5), PI587880A (Rpp1-b), PI594538A (Rpp1-b), and PI594723 (Rpp1-b). Rpp-pyramided lines carrying Rpp1-b + Rpp1-b, Rpp2 + Rpp1-b, Rpp4 + Rpp1-b, and single gene Rpp1-b were classi ed as "highly resistant". Furthermore, one sister line, 52117-57 (Rpp2 + Rpp1-b), showed immunity under eld conditions. The Rpppyramided genes are an alternative for achieving high resistance levels against ASR.

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Gene pyramiding combinations confer resistance of Asian soybean rust

Cited by 6 publications

References 74 publications

Soybean‐Phakopsora pachyrhizi interactions: towards the development of next‐generation disease‐resistant plants

Soybean‐Phakopsora pachyrhizi interactions: towards the development of next‐generation disease‐resistant plants

Quality and Agronomic Trait Analyses of Pyramids Composed of Wheat Genes NGli-D2, Sec-1s and 1Dx5+1Dy10

Rpp-Gene pyramiding confers higher resistance level to Asian soybean rust

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