Gene profiling of bone marrow- and adipose tissue-derived stromal cells: a key role of Kruppel-like factor 4 in cell fate regulation

Effect of low oxygen tension on the biological characteristics of human bone marrow mesenchymal stem cells

“…In stem cells, maintenance of proliferation is affected by molecular mechanisms mediated by stemness genes, such as KLF4, OCT4, and C-MYC. KLF4 prevents embryonic stem cell (ESC) differentiation (Zhang et al 2010) and maintains MSCs in an undifferentiated state (Saulnier et al 2011). Oct4 is a transcription factor essential for self-renewal and survival of MSCs (Tsai et al 2012), and C-MYC plays a vital role in cell proliferation and differentiation of adult stem cells (Bhandari et al 2011).…”

Thalidomide corrects impaired mesenchymal stem cell function in inducing tolerogenic DCs in patients with immune thrombocytopenia

“…Differentially expressed genes indexed by Gene Ontology (Go) biological process of MSCs were shown in the Gene Expression Omnibus database under accession number GSE44948; enrichment for variants was associated with DNA-dependent regulation of transcription, cell cycle, mitosis, DNA replication, cell proliferation, apoptosis, regulation of cell growth, and regulation of apoptosis, etc. Combining with previous research, 10,[30][31][32] we selected several genes associated with cell proliferation, apoptosis and cell cycle, including p27, p16, caspase-8, caspase-10, klf4, c-myc, oct3/4, and tgf-b1 which may be associated with THD modulation. As shown in Figure 2A-B, the expression of caspase-8 and caspase-10 was downregulated in THD-MSCs (ITP), while there was no significant difference between MSCs (control) and THD-MSCs (control).…”

An Adenosine Receptor-Krüppel-like Factor 4 Protein Axis Inhibits Adipogenesis

“…Considering our previous report of enhanced osteoblast differentiation by A2bAR signaling (70) and our current finding that activation of the A2bAR inhibits adipogenesis and increases the expression of KLF4, a stem cell factor, it follows that the A2bAR may be an important mediator of lineage determination. A comparison of the gene expression profiles of differentiated fibroblasts and human mesenchymal stem cells (bone marrow-and adipose tissue-derived) identified KLF4 as a key gene regulating stem cell fate (71). Therefore, our study suggests that signaling through the A2bAR is important in modulating KLF4 expression and, consequently, stem cell determination.…”