Gene mining in halophytes: functional identification of stress tolerance genes in <i>Lepidium crassifolium</i>

Rigó, Gábor; Valkai, Ildikó; Faragó, Dóra; Kiss, É; Houdt, Sara Van; Steene, Nancy Van de; Hannah, Matthew A.; Szabados, László

doi:10.1111/pce.12768

Cited by 23 publications

(19 citation statements)

References 50 publications

(57 reference statements)

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“…In Eutrema salsugineum (a halophyte, relative of Arabidopsis , also known as Thellungiella salsuginea and formerly Thellungiella halophila ), two functional genes responding to salt or heat stress were also identified with the FOX hunting system [ 65 , 66 ]. Also, in another Brassicaceae halophytic relative of Arabidopsis , Lepidium crassifolium , the cDNA library was screened with the FOX hunting system in Arbidopsis , and the proteins GDSL-like lipase and Acyl CoA binding protein 6 were supposed to be involved in salt tolerance and osmotic stress tolerance, respectively [ 67 ]. After a decade of development, this strategy has been identified as a powerful tool for elucidating functional genomics and facilitating the discovery of useful genes in model plants or crops [ 17 , 68 , 69 ] or digging useful candidate genes from some wild plants [ 65 , 66 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

“…Also, in another Brassicaceae halophytic relative of Arabidopsis , Lepidium crassifolium , the cDNA library was screened with the FOX hunting system in Arbidopsis , and the proteins GDSL-like lipase and Acyl CoA binding protein 6 were supposed to be involved in salt tolerance and osmotic stress tolerance, respectively [ 67 ]. After a decade of development, this strategy has been identified as a powerful tool for elucidating functional genomics and facilitating the discovery of useful genes in model plants or crops [ 17 , 68 , 69 ] or digging useful candidate genes from some wild plants [ 65 , 66 , 67 ]. The functional screening and identification of genes by transgenic assays in plants is a time-consuming and costly process during the first course of cDNA library screening.…”

Section: Discussionmentioning

confidence: 99%

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Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Zhang

Zheng

et al. 2018

IJMS

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Ipomoea pes-caprae is a seashore halophytic plant and is therefore a good model for studying the molecular mechanisms underlying salt and stress tolerance in plant research. Here, we performed Full-length cDNA Over-eXpressor (FOX) gene hunting with a functional screening of a cDNA library using a salt-sensitive yeast mutant strain to isolate the salt-stress-related genes of I. pes-caprae (IpSR genes). The library was screened for genes that complemented the salt defect of yeast mutant AXT3 and could grow in the presence of 75 mM NaCl. We obtained 38 candidate salt-stress-related full-length cDNA clones from the I. pes-caprae cDNA library. The genes are predicted to encode proteins involved in water deficit, reactive oxygen species (ROS) scavenging, cellular vesicle trafficking, metabolic enzymes, and signal transduction factors. When combined with the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses, several potential functional salt-tolerance-related genes were emphasized. This approach provides a rapid assay system for the large-scale screening of I. pes-caprae genes involved in the salt stress response and supports the identification of genes responsible for the molecular mechanisms of salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Zhang

Zheng

et al. 2018

IJMS

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“…First, potential genes from halophytes can be identified and used as candidates for improving salt tolerance in glycophytes. This can be achieved either by overexpression of identified gene of interest (reviewed in Himabindu et al, ) or by random approach wherein a cDNA library from halophyte is expressed in glycophyte and then cDNA inserts are identified in lines showing increased salt tolerance (Rigo et al, ). Second, halophyte cultivation is practiced for desalinization of degraded soils (Table ) and recovery of value‐added products (Table ).…”

Section: Halophyte Cultivation and Its Potential Applicationsmentioning

confidence: 99%

Halophytes in biosaline agriculture: Mechanism, utilization, and value addition

Srivastava²,

et al. 2017

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Land is considered as the life‐sustaining platform for food and water. However, there are contaminants such as salt, heavy metal, and industrial waste that decrease land fertility, posing serious threat to sustainable agriculture. In recent years, novel crop varieties with improved tolerance against environmental contaminants have been developed, but most of them face severe yield penalty. Alternatively, naturally tolerant plants such as extremophiles can be screened for their potential as crops. These crops should be tolerant to various abiotic stresses, perform better under extreme conditions and produce higher biomass and yield. In view of this, the present review focuses on the effects of saline soil on plants and how a class of plants termed as “halophytes” can tolerate high levels of salt. The potential applications of halophytes in phytoremediation, desalination, secondary metabolite production, medicine, food, and saline agriculture have been discussed. A concept of saline agriculture has been proposed for rehabilitation of saline and degraded lands. In this context, a potential halophyte is cultivated in salt‐contaminated soil for desalination. The harvested halophyte can have industrial value, and later on, rehabilitated soil can be utilized for agriculture purpose. Some success with halophyte cultivation has been demonstrated in environmentally degraded soils, and it is imperative that large‐scale adoption of halophytes, as potential candidates, can be accorded top priority for rehabilitating contaminated soils, which can pave way for sustainable agriculture.

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“…Random genes were transferred from L. crassifolium to A. thaliana , and it was observed that independent transgenic lines enhanced tolerance under several stress conditions (Rigó et al, 2016). Approximately 15% of functionally unknown genes were additionally expressed under salt stress compared to the non-stress conditions in M. crystallinum (Cushman and Bohnert, 2000; Kore-eda et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Halophytes: Potential Resources for Salt Stress Tolerance Genes and Promoters

2017

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Halophytes have demonstrated their capability to thrive under extremely saline conditions and thus considered as one of the best germplasm for saline agriculture. Salinity is a worldwide problem, and the salt-affected areas are increasing day-by-day because of scanty rainfall, poor irrigation system, salt ingression, water contamination, and other environmental factors. The salinity stress tolerance mechanism is a very complex phenomenon, and some pathways are coordinately linked for imparting salinity tolerance. Though a number of salt responsive genes have been reported from the halophytes, there is always a quest for promising stress-responsive genes that can modulate plant physiology according to the salt stress. Halophytes such as Aeluropus, Mesembryanthemum, Suaeda, Atriplex, Thellungiella, Cakile, and Salicornia serve as a potential candidate for the salt-responsive genes and promoters. Several known genes like antiporters (NHX, SOS, HKT, VTPase), ion channels (Cl−, Ca2+, aquaporins), antioxidant encoding genes (APX, CAT, GST, BADH, SOD) and some novel genes such as USP, SDR1, SRP etc. were isolated from halophytes and explored for developing stress tolerance in the crop plants (glycophytes). It is evidenced that stress triggers salt sensors that lead to the activation of stress tolerance mechanisms which involve multiple signaling proteins, up- or down-regulation of several genes, and finally the distinctive or collective effects of stress-responsive genes. In this review, halophytes are discussed as an excellent platform for salt responsive genes which can be utilized for developing salinity tolerance in crop plants through genetic engineering.

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Gene mining in halophytes: functional identification of stress tolerance genes in Lepidium crassifolium

Cited by 23 publications

References 50 publications

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Halophytes in biosaline agriculture: Mechanism, utilization, and value addition

Halophytes: Potential Resources for Salt Stress Tolerance Genes and Promoters

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