Gene mapping on mouse chromosome 8 by interspecific crosses: New data on a linkage group conserved on human chromosome 16q

Scherer, Gerd; Bausch, E.; Gaa, Andreas; O, von Deimling

doi:10.1016/0888-7543(89)90058-x

Cited by 28 publications

(4 citation statements)

References 34 publications

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“…For filter rehybridization, labeled TELO was removed from the filter in Fig. 3A using two 15 min washes with 0.1 x SSC in 0.5% SDS at 95-100~ This filter was rehybridized with MTI (Searle et al 1984), a cDNA probe to metallothioneine, a locus near the center of mouse Chr 8 (Ceci et al 1990;Scherer et al 1989). Fragment sizes in kb are indicated on the left.…”

Section: Mpmvmentioning

confidence: 99%

“…To determine whether the segregating Dde I fragments are close to the ends of their respective chromosomes, DNA from strain D was treated with Bal 31 for several time periods from 2-90 min, the enzyme was removed and the DNA repurified and digested with the endonuclease Dde I. The fragments were separated, blotted and probed with TELO or with MTI, a probe to the metallothioneine-1 (Mt-1) locus in the central region of Chr 8 (Ceci et al 1990;Scherer et al 1989). Brief treatment (2 min) with Bal 31 (Fig.…”

Section: Mpmvmentioning

confidence: 99%

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DNA variants with telomere probe enable genetic mapping of ends of mouse chromosomes

Elliott

Yen

1991

Mammalian Genome

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Dde I-digested DNA fragments from 11 inbred mouse strains were separated by electrophoresis, blotted and probed with a labeled oligomer, TELO, containing five repeats of the consensus mammalian telomere sequence, TTAGGG. Each strain produced a unique set of hybridizing fragments. Segregation analysis of TELO-hybridizing fragments from the BXD RI strains indicated that each fragment segregated as expected for a single gene. One fragment from strain DBA/2J was genetically linked to locus Xmv-9, previously mapped near the distal end of the map of chromosome (Chr) 4 and three fragments to Cck, near the distal end of Chr 9, suggesting that these fragments are telomeric and represent the ends of the chromosome maps. Confirmation of these map positions was obtained from a backcross. Fragments associated with the short arm of the Y Chr were found in DNA from strains C57BL/6J and DBA/2J. TELO-hybridizing fragments from DBA/2J were digested by the exonuclease Bal 31, under conditions in which fragments hybridizing to a cDNA probe for the metallothionein locus, located at the middle of mouse Chr 8, remained intact. Thus both biochemical and genetic tests indicate that several TELO-hybridizing fragments from Dde I-digested DNA are at the ends of chromosomes and probably derive from mouse telomeres. Using this approach should allow the mapping of genes relative to the ends of other mouse chromosomes.

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Section: Mpmvmentioning

confidence: 99%

Section: Mpmvmentioning

confidence: 99%

DNA variants with telomere probe enable genetic mapping of ends of mouse chromosomes

Elliott

Yen

1991

Mammalian Genome

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“…Nevertheless, genetic variations of MT2A deserve to be taken into consideration in the evaluation of thrombotic risk. Gene mapping showed that a large conserved linkage group exists on human chromosome 16q, including the loci for www.nature.com/scientificreports/ lecithin: cholesterol acyltransferase (LCAT), and metallothionein1 and 2 (Mt-1, -2) 26 . This may help partially explain the relationship between MT2A and LCAT mRNA levels observed in GEPIA.…”

Section: Discussionmentioning

confidence: 99%

Association of metallothionein 2A rs10636 with low mean corpuscular volume (MCV), low mean corpuscular haemoglobin (MCH) in healthy Taiwanese

Chen

Pan

et al. 2023

Sci Rep

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Human metallothionein-2A (MT2A) protein participates in metal homeostasis, detoxification, oxidative stress reduction, and immune defense. It decreases heavy metal ions and reactive oxygen species (ROS) during injury of cells and tissues. The single nucleotide polymorphisms at the MT2A gene have been associated in various human diseases including cancer. The current study aimed to elucidate associations between MT2A genotypes with the clinical, biochemical, and molecular characteristics that potentially related to lowered MT2A ex-pression. One hundred and forty-one healthy Taiwanese subjects were enrolled from Changhua Show-Chwan Memorial Hospital. Clinical, biochemical and molecular characteristics including the frequent minor allele SNPs, rs28366003 and rs10636, within the MT2A gene were determined. The genotype distribution of MT2A rs10636 fits the Hardy–Weinberg equilibrium. The significant associations with gradually decline of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were identified with MT2A rs10636 and rs28366003 using analysis of variance (ANOVA) with Tukey’s analysis as a post hoc test. We further validated the correlations between the expressions of genes in erythropoiesis, cholesterol synthesis, platelet synthesis, insulin with MT2A using the web-based Gene Expression Profiling Interactive Analysis (GEPIA) databases. The results revealed that hypoxia-inducible factor 1α (HIF-1α), erythropoietin (EPO), lipoprotein lipase (LPL), and lecithin-cholesterol acyltransferase (LCAT) mRNA ex-pression are significantly correlated with MT2A mRNA expression. In conclusion, these results suggested that genetic variations of MT2A rs10636 and rs28366003 might be an important risk factor for erythropoiesis in the Taiwanese general population.

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“…Combining these data with the results of this study gives the gene order on human 16q as cen-MT-LCAT-UVO-HP-TAT-CTRB-APRT-qter. The corresponding loci within the conserved linkage group are arranged in apparently the same order on the genetic map of mouse chromosome 8 (Scherer et al, 1989). Notably, the close physical location of LCAT and UVO at proximal 16q22.1 corresponds toaclose genetic linkage (0/101 recombinants) of the corresponding loci.…”

Section: Discussionmentioning

confidence: 99%

Regional assignment of the human loci for uvomorulin (UVO) and chymotrypsinogen B (CTRB) with the help of two overlapping deletions on the long arm of chromosome 16

Natt

Magenis

Zimmer

et al. 1989

Cytogenet Genome Res

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Using cDNA probes for the human uvomorulin (UVO) and rat chymotrypsinogen B (CTRB) genes, we have analyzed two overlapping interstitial deletions on human chromosome 16q by Southern blot analysis. One deletion, with breakpoints at 16q22.1 and 16q22.3, results in loss of the UVO locus The second deletion, whose breakpoints are at 16q22.1 and 16q23.2, leads to loss of the CTRB locus. Therefore, UVO resides between both proximal deletion breakpoints within band 16q22.1 whereas CTRB is located between both distal breakpoints at 16q22.3 and 16q23.2.

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Gene mapping on mouse chromosome 8 by interspecific crosses: New data on a linkage group conserved on human chromosome 16q

Cited by 28 publications

References 34 publications

DNA variants with telomere probe enable genetic mapping of ends of mouse chromosomes

DNA variants with telomere probe enable genetic mapping of ends of mouse chromosomes

Association of metallothionein 2A rs10636 with low mean corpuscular volume (MCV), low mean corpuscular haemoglobin (MCH) in healthy Taiwanese

Regional assignment of the human loci for uvomorulin (UVO) and chymotrypsinogen B (CTRB) with the help of two overlapping deletions on the long arm of chromosome 16

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