Gene mapping and sequence analysis of the unique short region of the simian herpesvirus SA 8 genome

Abstract

A 10.5 kbp BamHI restriction fragment representing most of the unique short (Us) region of the genome of the simian alpha-herpesvirus SA8 was identified and cloned. Partial sequencing of this DNA fragment identified regions of sequence homology with eight open reading frames (ORFs) of HSV1 and/or HSV2. Sequence and size analysis of subcloned fragments of the SA8 Us region and comparison with homologous HSV Us sequences determined that the number, order, size, and orientation of SA8 Us ORFs are comparable to those of HSV. Based on the location of transcriptional control elements, transcription of SA8 Us genes appears to be organized into 3' co-terminal mRNA sets as in HSV, although the grouping of the gene sets is different. The SA8 US4 (gG) ORF is more similar to that of HSV2 than HSV1, both in size and predicted amino acid sequence. Complete sequences were determined for five SA8 genes which represent homologs of the HSV gD, gE, gI, US5, and US9 genes. The predicted polypeptides encoded by SA8 are similar to the corresponding HSV polypeptides. All SA8 Us genes were more closely related to those of HSV than to related gene homologs of other mammalian alpha-herpesviruses.

Complete nucleotide sequence of the herpesvirus simiae glycoprotein G gene and its expression as an immunogenic fusion protein in bacteria

“…Sequencing within the SHBV U s region has provided the full nucleotide sequences for SHBV equivalents of the gJ and gD genes and the incomplete sequence of the gI gene (Bennett et al, 1992); these genes are collinear with their HSV homologues. This further confirmed the close molecular relationship between HSV and SHBV, while similar findings have been made for related glycoprotein genes in the U s region of the SA8 genome (Eberle et al, 1993).…”

“…SHBV gG contains the three conserved cysteines and an adjacent N-glycosylation site which similarly occur in most other alphaherpesvirus gGs (Rea et al, 1985;McGeoch, 1990;Colle et al, 1992;Crabb et al, 1992, Telford et al, 1992Eberle et al, 1993;Kongsuwan et al, 1993;Spatz et al,, 1994). SHBV gG and gG-2 both possess an identical octapeptide APQSLLVG (residue 269 in SHBV gG; Fig.…”

“…1) which shared 74.7 % similarity with the C termini of the protein kinase encoded by the US3 gene of HSV-1 and -2 (McGeoch et al, 1985(McGeoch et al, , 1987. Alignment with the US3 homologue of SA8 (Eberle et at., "1993) The complete ORF (Fig. 1) encoded a 593 amino acid protein and was located between the protein kinase and the gJ genes, the position occupied by the US4 (gG) gene in HSV-1 and -2.…”

“…Glycoprotein G-1 and gG-2 sequences are from McGeoch et al (1985McGeoch et al ( , 1987. Partial sequencing of SA8 gG has covered only the N and C termini (Eberle et al, 1993). The vertical arrow indicates residue 236, which is the first SHBV gG amino acid in the fusion protein expressed from recombinant pEX358C.…”

Equine herpesvirus-4 glycoprotein G is secreted as a disulphide-linked homodimer and is present as two homodimeric species in the virion.

“…Of these, only four, gB, gD and the gH\gL complex, are essential for replication in vitro in cell culture, although it is presumed that the remainder serve important roles in vivo in viral pathogenesis. Glycoprotein G (gG) homologues have been identified in most alphaherpesviruses that have been examined (Colle et al, 1992 ;Crabb et al, 1992 ;Eberle et al, 1993 ;Ficorilli et al, 1995 ;Fukushi et al, 1997 ;Keil et al, 1996 ;Kongsuwan et al, 1993 ;McGeoch et al, 1987 ;Rea et al, 1985 ;Slomka et al, Fax j613 9416 2616. e-mail heidid!ariel.its.unimelb.edu.au associated species, gGV L and gGV S , are present in the virion as 140 and 20 kDa proteins, respectively. As gGS and gGV L do not appear to associate stably with other viral proteins, it is most likely that each species exists as a disulphide-linked homodimer.…”