Gene injections of vascular endothelial growth factor and growth differentiation factor-9 stimulate ovarian follicular development in immature female rats

Shimizu, Takashi; Izutsu, Koji; Ogawa, Yoshinori; Miyazaki, Hitoshi; Sasada, Hiroshi; Sato, Eimei

doi:10.1016/j.fertnstert.2007.06.043

Cited by 13 publications

(9 citation statements)

References 33 publications

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“…Spicer et al (2008) reported that bovine TC isolated from small follicles were more responsive to GDF9 than their counterparts isolated from larger follicles, and that GDF9 stimulated proliferation, while inhibiting progesterone and androstenedione production, by the TC of small antral follicles. Similarly, building on the observations of Vitt et al (2000) in the rat suggesting that GDF9 controls proliferation of GC, Shimizu et al (2008) reported that intra-ovarian injection of GDF9 promoted the development of medium-sized antral follicles. Collectively, these observations in other species suggest that GDF9 modulates aspects of GC and TC function important for follicle selection.…”

Section: Expression Of Bmps and Bmpr In Pig Folliclesmentioning

confidence: 92%

Temporal regulation of BMP2, BMP6, BMP15, GDF9, BMPR1A, BMPR1B, BMPR2 and TGFBR1 mRNA expression in the oocyte, granulosa and theca cells of developing preovulatory follicles in the pig

Paradis

Novak²,

Murdoch³

et al. 2009

Reproduction

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This study aimed to describe the abundance and localization of BMP2, BMP6, BMP15, GDF9, BMPR1A, BMPR1B, BMPR2 and TGFBR1 mRNA during pig preovulatory follicular development and to evaluate their implication in improving follicular maturity in the preovulatory period preceding the second versus first post-weaning oestrus. Oocytes, granulosa (GC) and theca cells (TC) were recovered from antral follicles of primiparous sows at day 1, 2 and 4 after weaning and at day 14, 16 and 20 of their subsequent oestrous cycle. Realtime PCR analysis revealed that with the exception of BMP6 mRNA, which was absent in GC, all genes were expressed in every cell type. Although BMP6, BMP15 and GDF9 mRNA were most abundant in the oocyte, their expression remained relatively constant during follicular development. By contrast, receptor BMPR1B and TGFBR1 expressions in the GC and TC were temporally regulated. BMPR1B mRNA abundance was positively correlated with plasma oestradiol (E 2 ) suggesting that its regulation by oestrogen may be implicated in normal folliculogenesis. Interestingly, the increase in BMPR1B mRNA and protein abundance during the periovulatory period in GC and TC suggests a role for bone morphogenetic protein (BMP) 15 in the ovulatory process. Finally, expression of these ligands and receptors was not associated with potential differences in follicle maturity observed during the second versus first post-weaning preovulatory follicular wave. In conclusion, our results clearly demonstrate the presence of a complex signalling system within the pig follicle involving the transforming growth factor-b superfamily and their receptors, and provide evidence to support a role for BMP15 and BMPR1B during ovulation.

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Section: Expression Of Bmps and Bmpr In Pig Folliclesmentioning

confidence: 92%

Temporal regulation of BMP2, BMP6, BMP15, GDF9, BMPR1A, BMPR1B, BMPR2 and TGFBR1 mRNA expression in the oocyte, granulosa and theca cells of developing preovulatory follicles in the pig

Paradis

Novak²,

Murdoch³

et al. 2009

Reproduction

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“…Permissive selection for further growth and development from primary follicle pools is instead dependent upon GDF‐9. The available evidence suggests that GDF‐9 can increase the number of medium‐sized antral follicles in mice, especially when acting in conjunction with vascular endothelial growth factor (VEGF) (Shimizu, ; Shimizu et al, ; Cerrillo et al, ). This relationship is highlighted by the fact that although intra‐ovarian injections of cDNA encoding each cytokine can stimulate follicular growth in mice, these cytokines synergise when administered together, resulting in more oocytes being ovulated (Shimizu et al, ).…”

Section: The Primordial‐to‐primary Follicle Transitionmentioning

confidence: 99%

“…The available evidence suggests that GDF‐9 can increase the number of medium‐sized antral follicles in mice, especially when acting in conjunction with vascular endothelial growth factor (VEGF) (Shimizu, ; Shimizu et al, ; Cerrillo et al, ). This relationship is highlighted by the fact that although intra‐ovarian injections of cDNA encoding each cytokine can stimulate follicular growth in mice, these cytokines synergise when administered together, resulting in more oocytes being ovulated (Shimizu et al, ). GDF‐9 can also stimulate pre‐antral follicle growth, promote granulosa‐cumulus cell phenotypic transition, and suppress progesterone production by cells in mice, rats, and cattle (Hayashi et al, ; Elvin et al, ,; Vitt et al, ; Juengel et al, ; Spicer et al, ).…”

Section: The Primordial‐to‐primary Follicle Transitionmentioning

confidence: 99%

Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation

Field

Dasgupta

Cummings

et al. 2013

Molecular Reproduction Devel

161

110

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SUMMARYCytokines are key regulators of ovarian physiology, particularly in relation to folliculogenesis and ovulation, where they contribute to creating an environment supporting follicle selection and growth. Their manifold functions include regulating cellular proliferation/differentiation, follicular survival/atresia, and oocyte maturation. Several cytokines, such as TGF-b-superfamily members, are involved at all stages of folliculogenesis while the production of others is stage-dependent. This review draws upon evidence from both human and animal models to highlight the species-specific roles at each milestone of follicular development. Given these pivotal roles and their ease of detection in follicular fluid, cytokines have been considered as attractive biomarkers of oocyte maturational status and of successful assisted reproductive outcome. Despite this, our understanding of cytokines and their interactions remains incomplete, and is still frequently limited to overly simplistic descriptions of their interrelationships. Given our increased appreciation of cytokine activity in complex and highly regulated networks, we put forward the case for using Bayesian modelling approaches to describe their hierarchical relationships in order to predict causal physiological interactions in vivo.Mol. Reprod. Dev. 81: 284À314,

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“…To test this hypothesis, we performed in vivo injection of VEGF gene fragments into pig ovaries in an attempt to over-produce VEGF and enhance the thecal angiogenesis associated with follicular development. 39 – 42 ) Histologic examination revealed that the vascular density in the theca interna of follicles in VEGF-treated ovaries increased two-fold compared with that in untreated ovaries. The pattern of perifollicular angiogenesis in the VEGF-treated group was essentially the same as that during follicular development after treatment with equine chorionic gonadotropin (eCG).…”

Section: The Mechanism Controlling Selective Follicular Development Imentioning

confidence: 99%

Intraovarian control of selective follicular growth and induction of oocyte maturation in mammals

Sato

2015

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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In newborn mammals, most of the germ cell population rests in a pool of quiescent small follicles in the ovaries. Regularly throughout adulthood, a small percentage of these oocytes and follicles grows to a certain stage of development and then either degenerates or matures and ovulates. This entire process is under both exogenous and endogenous control. Recent work, including my laboratory’s, has clarified that cytokines and glycosaminoglycans are involved as exogenous and endogenous factors in ovarian follicular development, atresia, and maturation in mammals. The present article describes our contribution regarding the cytokines and ovarian glycosaminoglycans that act as intraovarian regulators of follicular development and oogenesis, including oocyte maturation, in mammals.

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Gene injections of vascular endothelial growth factor and growth differentiation factor-9 stimulate ovarian follicular development in immature female rats

Cited by 13 publications

References 33 publications

Temporal regulation of BMP2, BMP6, BMP15, GDF9, BMPR1A, BMPR1B, BMPR2 and TGFBR1 mRNA expression in the oocyte, granulosa and theca cells of developing preovulatory follicles in the pig

Temporal regulation of BMP2, BMP6, BMP15, GDF9, BMPR1A, BMPR1B, BMPR2 and TGFBR1 mRNA expression in the oocyte, granulosa and theca cells of developing preovulatory follicles in the pig

Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation

Intraovarian control of selective follicular growth and induction of oocyte maturation in mammals

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