Gene induction by desiccation stress in the entomopathogenic nematode Steinernema carpocapsae reveals parallels with drought tolerance mechanisms in plants

Tyson, Trevor; Reardon, Wesley; Browne, John A.; Burnell, Ann M.

doi:10.1016/j.ijpara.2006.12.015

Cited by 32 publications

(22 citation statements)

References 80 publications

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“…Of the recognisable sequences, those in the stress and protein homeostasis categories are likely to be the most relevant to anhydrobiosis, including LEA protein, antioxidant, DNA repair, molecular chaperone and protein clearance genes. Therefore, although we are clearly only sampling a subset of the desiccome, bdelloids follow a pattern of gene expression observed in other dehydrating anhydrobiotes, principally nematodes (Adhikari et al, 2009;Gal et al, 2003;Reardon et al, 2010;Tyson et al, 2007) and tardigrades (Mali et al, 2010;Schill et al, 2004). Nevertheless, evidence for the involvement of non-reducing disaccharides in bdelloid rotifers is still lacking.…”

Section: Discussionmentioning

confidence: 91%

“…Thus, experimental approaches that identified genes upregulated by dehydration were first established in the resurrection plants (Bartels, 2005;Illing et al, 2005) and have more recently been implemented in invertebrates, primarily in tardigrades (Mali et al, 2010) and nematodes (Gal et al, 2003;Reardon et al, 2010;Tyson et al, 2007). Studies of this type could be particularly informative in bdelloid rotifers, as the anhydrobiology of these aquatic micro-invertebrates has unusual aspects: besides their lack of non-reducing disaccharides, the bdelloid LEA proteins defined to date are not especially hydrophilic and can exhibit secondary structure in solution (Pouchkina-Stantcheva et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciae

Boschetti

Pouchkina-Stantcheva

Hoffmann

et al. 2011

Journal of Experimental Biology

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SUMMARYBdelloid rotifers are aquatic micro-invertebrates with the ability to survive extreme desiccation, or anhydrobiosis, at any life stage. To gain insight into the molecular mechanisms used by bdelloids during anhydrobiosis, we constructed a cDNA library enriched for genes that are upregulated in Adineta ricciae 24h after onset of dehydration. Resulting expressed sequence tags (ESTs) were analysed and sequences grouped into categories according to their probable identity. Of 75 unique sequences, approximately half (36) were similar to known genes from other species. These included genes encoding an unusual group 3 late embryogenesis abundant protein, and a number of other stress-related and DNA repair proteins. Open reading frames from a further 39 novel sequences, without counterparts in the database, were screened for the characteristics of intrinsically disordered proteins, i.e. hydrophilicity and lack of stable secondary structure. Such proteins have been implicated in desiccation tolerance and at least five were found. The majority of the genes identified was confirmed by real-time quantitative PCR to be capable of upregulation in response to evaporative water loss. Remarkably, further database and phylogenetic analysis highlighted four ESTs that are present in the A. ricciae genome but which represent genes probably arising from fungi or bacteria by horizontal gene transfer. Therefore, not only can bdelloid rotifers accumulate foreign genes and render them transcriptionally competent, but their expression pattern can be modified for participation in the desiccation stress response, and is presumably adaptive in this context. Supplementary material available online at

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciae

Boschetti

Pouchkina-Stantcheva

Hoffmann

et al. 2011

Journal of Experimental Biology

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“…One recurring theme is the presence of a number of abundant, highly hydrophilic proteins in desiccation-tolerant organisms (e.g., ref. 29), chief among which are the LEA proteins (5,6).…”

Section: Discussionmentioning

confidence: 99%

Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization function

Chakrabortee

Boschetti

Walton

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

277

248

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The ability of certain plants, invertebrates, and microorganisms to survive almost complete loss of water has long been recognized, but the molecular mechanisms of this phenomenon remain to be defined. One phylogenetically widespread adaptation is the presence of abundant, highly hydrophilic proteins in desiccation-tolerant organisms. The best characterized of these polypeptides are the late embryogenesis abundant (LEA) proteins, first described in plant seeds >20 years ago but recently identified in invertebrates and bacteria. The function of these largely unstructured proteins has been unclear, but we now show that a group 3 LEA protein from the desiccation-tolerant nematode Aphelenchus avenae is able to prevent aggregation of a wide range of other proteins both in vitro and in vivo. The presence of water is essential for maintenance of the structure of many proteins, and therefore desiccation stress induces unfolding and aggregation. The nematode LEA protein is able to abrogate desiccation-induced aggregation of the water-soluble proteomes from nematodes and mammalian cells and affords protection during both dehydration and rehydration. Furthermore, when coexpressed in a human cell line, the LEA protein reduces the propensity of polyglutamine and polyalanine expansion proteins associated with neurodegenerative diseases to form aggregates, demonstrating in vivo function of an LEA protein as an antiaggregant. Finally, human cells expressing LEA protein exhibit increased survival of dehydration imposed by osmotic upshift, consistent with a broad protein stabilization function of LEA proteins under conditions of water stress.anhydrobiosis ͉ late embryogenesis abundant protein W ater is essential for life, but some organisms survive desiccation and the dry state for long periods during which metabolism and life processes come to a halt, but resume on rehydration. Desiccation tolerance, or anhydrobiosis (''life without water''), is found across all biological kingdoms, including animals and plants such as the nematode Aphelenchus avenae and the resurrection plant Craterostigma plantagineum (1-3). Investigations into the molecular mechanisms of desiccation tolerance have highlighted the importance of various hydrophilic proteins, chief among which are the late embryogenesis abundant (LEA) proteins (4).LEA proteins have been known for many years to accumulate in maturing plant seeds as they acquire desiccation tolerance (5, 6), but their discovery in invertebrates (7-13) suggests that similar mechanisms govern anhydrobiosis in both animals and plants. LEA proteins are known to be largely unstructured in solution, probably because their extreme hydrophilicity favors association with water over intrachain interactions, but they can show increased folding when dried or when associated with phospholipid bilayers (14-16). Although LEA proteins are widely held to protect cells against water stress, their precise role has been a puzzle since they were first described. Recently, evidence supporting possible functions has bee...

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“…Animals for which LEA-like proteins have been reported are nematodes (17,(34)(35)(36)(37)(38), rotifers (39)(40)(41), embryos of the brine shrimp Artemia franciscana (42)(43)(44)(45), collembolan species including the arctic springtail Megaphorura arctica (46,47), the chironomid larva Polypedilum vanderplanki (48), and tardigrades (as referenced to an EST library, (49)). …”

Section: Distribution and Expression Of Lea Proteins In Animalsmentioning

confidence: 99%

LEA Proteins During Water Stress: Not Just for Plants Anymore

Hand

Menze

Toner

et al. 2011

Annu. Rev. Physiol.

353

340

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Gene induction by desiccation stress in the entomopathogenic nematode Steinernema carpocapsae reveals parallels with drought tolerance mechanisms in plants

Cited by 32 publications

References 80 publications

Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciae

Foreign genes and novel hydrophilic protein genes participate in the desiccation response of the bdelloid rotifer Adineta ricciae

Hydrophilic protein associated with desiccation tolerance exhibits broad protein stabilization function

LEA Proteins During Water Stress: Not Just for Plants Anymore

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