Gene ilvY of Salmonella typhimurium

Blazey, D L; Burns, R. O.

doi:10.1128/jb.142.3.1015-1018.1980

Cited by 13 publications

(6 citation statements)

References 15 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The existence of the regulatory gene 1t' Y in S. typhimurium has been demonstrated by cistrans analysis although ilvY could not be conclusively mapped with respect to ilvA and ilC (4). The presence of illY on pDU1 was determined by F factor mobilization of the chimeric plasmid to the S. typhimurium strain DU702 iltA ilv'Y.…”

Section: Resultsmentioning

confidence: 98%

See 1 more Smart Citation

Molecular cloning and expression of the ilvGEDAY genes from Salmonella typhimurium

Blazey¹,

Kim²,

Burns³

1981

J Bacteriol

Self Cite

View full text Add to dashboard Cite

The ilvGEDAY genes of Salmonella typhimurium were cloned in Escherichia coli K-12 by in vitro recombination techniques. A single species of recombinant plasmid, designated pDU1, was obtained by selecting for Valr Ampr transformants of strain SK1592. pDU1 was shown to contain a 14-kilobase EcoRI partial digestion product of the S. typhimurium chromosome inserted into the EcoRI site of the pVH2124 cloning vector. The ilvGEDAY genes were found to occupy a maximum length of 7.5 kilobases. Restriction endonuclease analysis of the S. typhimurium ilv gene cluster provided another demonstration of the gene order as well as established the location of ilv Y between ilvA and ilvC. The presence of a ribosomal ribonucleic acid operon on the pDU1 insert, about 3 kilobases from the 5' end of ilvG, was shown by Southern hybridization. The expression of the ilvGEDA operon from pDU1 was found to be elevated, reflecting the increased gene dosage of the multicopy plasmid. A polarity was observed with respect to ilvEDA expression which is discussed in terms of the possible translational effects of the two internal promoter sequences, one located proximal to ilvE and the other located proximal to ilvD.

show abstract

Section: Resultsmentioning

confidence: 98%

“…The RNAs were 32P labeled with polynucleotide kinase by the method of Richardson (31). rRNA (0.4 mg) and tRNA (0.02 mg) each were reacted with 0.12 nmol of [y-_32P]ATP with a specific activity of 4,200 Ci/mmol. Southern (36).…”

Section: Vol 147 1981mentioning

confidence: 99%

Molecular cloning and expression of the ilvGEDAY genes from Salmonella typhimurium

Blazey¹,

Kim²,

Burns³

1981

J Bacteriol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cis-dominant effect of ilvY::TnJO or ilvY::Mu insertion mutations on ilvC expression was explained by the ability of these elements to effect termination of transcription initiated at the ilvY promoter. Two S. typhimurium ilvY mutants have been described that arose by imprecise excision of TnJO from ilvA::TnJO insertion mutations (5,6). Chromosomal alterations associated with imprecise excision of TnWO are either inversions or deletions (13).…”

Section: Resultsmentioning

confidence: 99%

“…ilvA::TnJO-generated mutations affecting ilvY expression would necessarily disrupt the association between py and ilvC by inverting py or deleting py. However, both S. typhimurium ilvY mutants could be complemented in trans by appropriate F' ilv or recombinant plasmids (5,6). In view of the apparent difference between the S. typhimurium and E. coli K-12 ilvY-ilvC systems, we used the S. typhimurium ilv YC-galK plasmids to define further the relationship between ilv Y and ilvC.…”

Section: Resultsmentioning

confidence: 99%

“…The S. typhimurium ilvY gene was mapped between ilvA and ilvC by analysis of cloned fragments of the gene cluster (6). The ilvY gene product has an essential role in expression of ilvC as demonstrated by the isolation of an ilv Y mutant that displays a trans-recessive IlvCphenotype (5). The ilv YC genes constitute a distinctive regulatory system that employs positive control, which is uncommon for biosynthetic genes (21) and sharply contrasts with the control of the ilvGEDA operon.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Regulation of Salmonella typhimurium ilvYC genes

Blazey¹,

Burns²

1984

J Bacteriol

Self Cite

View full text Add to dashboard Cite

The Salmonella typhimurium LT2 ilvYC genes were studied by fusion of each gene to the Escherichia coli K-12 galK gene. The expression of ilvY and ilvC could then be determined by measurement of the galK-encoded galactokinase enzyme. The promoter for ilvC, Pc, was located by this technique to a 0.42-kilobase BglII-EcoRI fragment of the S. typhimurium ilvGEDAYC gene cluster. This sequence was completely sufficient for aLacetohydroxyacid-inducible galK expression. The ilvY gene was located within a 1.0-kilobase XhoI-SalI fragment. ilvY gene expression was constitutive with respect to ilv-specific control signals. The ilvY gene was transcribed in the same direction as the other two transcriptional units in the ilvGEDAYC gene cluster, ilvGEDA and ilvC. Transcription of the ilvC gene was completely dependent upon the activity of its own promoter, Pc, and independent from transcription of the ilvY gene. The role of the intervening region between ilvY and ilvC in regulation of ilvC expression was explored.

show abstract

Control of arg gene expression in salmonella typhimurium by the arginine repressor from Escherichia coli K-12

1983

View full text Add to dashboard Cite

The regulation of synthesis of arg enzymes in Salmonella typhimurium by the arginine repressor of Escherichia coli K-12 has been reevaluated using a strain of S. typhimurium in which the argR gene was rendered nonfunctional by inserting the translocatable tetracycline-resistance element Tn10 into the argR gene. In contrast to previous studies, the introduction of the argR+ allelle of E. coli on an F-prime factor to the argR::Tn10 S. typhimurium strain reduced the synthesis of arg enzymes to essentially wild-type levels. The elevated levels of arg enzymes observed in other hybrid merodiploids may have been the consequence of the formation of hybrid repressor molecules. The readily scoreable phenotype of tetracycline resistance facilitated establishing linkage of cod and argR (0.6% cotransduction) by P22 phage-mediated transduction.

show abstract

Gene ilvY of Salmonella typhimurium

Abstract: Evidence is presented for the existence in Salmonella typhimurium LT2 of the regulatory gene ilvY. The Escherichia coli K-12 ilvY gene product is shown to complement a S. typhimurium ilvY mutation in vivo.

Cited by 13 publications

References 15 publications

Molecular cloning and expression of the ilvGEDAY genes from Salmonella typhimurium

Molecular cloning and expression of the ilvGEDAY genes from Salmonella typhimurium

Regulation of Salmonella typhimurium ilvYC genes

Control of arg gene expression in salmonella typhimurium by the arginine repressor from Escherichia coli K-12

Contact Info

Product

Resources

About