“…As can be seen in Figure 4B, the amount of newly synthesized 5.8S RNA was the same in oocytes that had been injected with oligonucleotide 61-75 (lane 2) as in control oocytes that were injected with an unrelated oligonucleotide (lane 1) or not injected with any oligonucleotide (lanes 3 and 4). The doublet band seen for 5.8S RNA reflects heterogeneity of a few nucleotides at the 5' end of both 5.8S RNA and its 32S RNA precursor, as previously reported for Xenopus (Boseley et al, 1978;Ford and Mathieson, 1978), mouse (Bowman et al, 1983), rat (Smith et al, 1984), HeLa cells (Khan and Maden, 1977) and yeast (Veldman et al, 1980). does not affect the amount of newly synthesized 5.8S, 18S and 28S rRNAs relative to controls.…”