Gene Flow in Plant Pathosystems

McDermott, Joseph M; McDonald, Bruce A.

doi:10.1146/annurev.py.31.090193.002033

Cited by 517 publications

(232 citation statements)

References 54 publications

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“…The amount of genetic variation within a population indicates how rapidly a pathogen can evolve and thus provides valuable information in predicting the effectiveness of control measures such as host resistance genes or chemical control (McDermott and McDonald 1993). The main evolutionary forces that contribute to the genetic structure within and between populations are gene flow, genetic drift, reproduction/mating systems, population size, and selection.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers

et al. 2010

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Knowledge of genetic diversity in A. rabiei provides different levels of information that are important in the management of crop germplasm resources. Gene flow on a regional level indicates a significant potential risk for the regional spread of novel alleles that might contribute to fungicide resistance or the breakdown of resistance genes. Simple sequence repeat (SSR) and mating type (MAT) markers were used to determine the genetic structure, and estimate genetic diversity and the prevalence of mating types in 103 Ascochyta rabiei isolates from seven counties in the Ilam and Kermanshah provinces of western Iran (Ilam, Aseman abad, Holaylan, Chardavol, Dareh shahr, Gilangharb, and Sarpul). A set of 3 microsatellite primer pairs revealed a total of 75 alleles; the number of alleles varied from 15 to 34 for each marker. A high level of genetic variability was observed among A. rabiei isolates in the region. Genetic diversity was high (He=0.788) within populations with corresponding high average gene flow and low genetic distances between populations. The smallest genetic distance was observed between isolates from Ilam and Chardavol. Both mating types were present in all populations, with the majority of the isolates belonging to Mat1-1 (64%), but within populations the proportions of each mating type were not significantly different from 50%. Results from this study will be useful in breeding for Ascochyta blight-resistant cultivars and developing necessary control measures.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers

et al. 2010

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“…Estimate of gene flow (Nm) was calculated as Nm=0.5×(1−Gst)/Gst (McDermott and McDonald 1993), where Gst is the gene differentiation. Analysis of molecular variance (AMOVA) input files were created using AMOVA-PREP version 1.01 (Miller 1998) and the analyses were made using WinAMOVA version 1.5 (Excoffier et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of RAPD, ISSR and DAMD markers in assessment of genetic variability and population structure of wild Musa acuminata colla

Lamare

Rao

2015

Physiol Mol Biol Plants

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North east India is considered as one of the major biodiversity hotspots worldwide and centre of origin of several plant species including Musa. Musa acuminata Colla is known to be one of the wild progenitors of cultivated bananas and plantains. Three single primer based DNA marker techniques viz., random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and directed amplification of minisatellites DNA (DAMD) were used for diversity diagnostics among 25 genotypes of wild M. acuminata collected from Meghalaya province of north east India. A total of 58 primers (26-RAPD, 21-ISSR, and11-DAMD) yielded 451 DNA fragments, of which 395 (87.58 %) were found to be polymorphic in nature. The polymorphic information content (PIC) values were almost identical for each marker system. The resolving power of the marker system was found to be highest in RAPD (3.96) whereas ISSR resolved highest marker index (16.39) in the study. Selected amplicon data obtained through single primer amplification reactions were utilized for determination of diversity within and among the populations of M. acuminata. Nei's genetic differentiation (Gst) value (0.451) indicated higher proportion of the genetic variation within the populations which is supported by the AMOVA analysis (88 %). The study provides insight into the efficacy of RAPD, ISSR and DAMD to analyse the genetic variation existing in the wild Musa germplasm, which can further be exploited for quality trait improvement and domestication of such important horticultural crops. The genetic diversity based population structure may shed light on the genetic basis of speciation and evolution of various species within the genus Musa.

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“…Analysis of multiple locations in Popgene (Nei 1973, McDermott andMcDonald 1993) revealed a moderately high level of differentiation (G st ϭ 0.5032) (Table 3). G st values within groups were variable, although it was slightly lower between locations in newly established territory (G st ϭ 0.4194) than among locations within the historical range (G st ϭ 0.5121).…”

Section: Resultsmentioning

confidence: 99%

Population Genetics of the Western Bean Cutworm (Lepidoptera: Noctuidae) Across the United States

Lindroth¹,

Hunt²,

Skoda

et al. 2012

Annals of the Entomological Society of America

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The western bean cutworm, Striacosta albicosta (Smith), is a secondary pest of maize (Zea mays L.) and dry beans (Phaseolus vulgaris L.) in the western United States. Recently, this insect has undergone a major territory expansion into the eastern United States and has become a pest throughout much of the Corn Belt. This study was instigated to examine the population genetics of this pest to facilitate control and resistance management, as well as to shed light on the current habitat expansion. S. albicosta individuals were collected from 24 different locations across the traditional and expanded range and amplified fragment length polymorphism analysis was conducted to assess genetic variability. In total, 90 markers were analyzed, encompassing >90% of genetic variation. Gst across all locations was moderately high (Gst = 0.5032). AMOVA analysis revealed that the majority of genetic variation was within locations (54%) and among locations within groups (45%) indicating genetic differentiation of subpopulations. The Mantel test revealed no correlation between geographic and genetic distance (n = 548; r = 0.0045; P = 0.4350). Locations sampled in the eastern United States did not exhibit any reduction in genetic variation in comparison to locations sampled in the western United States, so we conclude that no bottleneck event has occurred with this territory expansion.

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Gene Flow in Plant Pathosystems

Cited by 517 publications

References 54 publications

Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers

Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers

Efficacy of RAPD, ISSR and DAMD markers in assessment of genetic variability and population structure of wild Musa acuminata colla

Population Genetics of the Western Bean Cutworm (Lepidoptera: Noctuidae) Across the United States

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