Gene families of maize glutathione–ascorbate redox cycle respond differently to abiotic stresses

Liu, Yun-Jun; Yuan, Yuan; Liu, Yanyan; Liu, Yan; Fu, Junjie; Zheng, Jun; Wang, Guoying

doi:10.1016/j.jplph.2011.08.018

Cited by 50 publications

(60 citation statements)

References 43 publications

(74 reference statements)

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“…Increases in APX activity and transcript expression in the foliar tissues of higher plants exposed to osmotic treatment have been shown in previous studies [18,59]. In this study, the transcripts of different ZmAPX genes showed varying responses in the maize seedlings treated with 15% PEG-6000 (Fig 8).…”

Section: Discussionsupporting

confidence: 82%

“…It has been reported that MDHAR overexpression can increase the resistance to oxidative stress [52,53]. In addition, previous studies have shown that the overexpression of DHAR genes is beneficial in elevating tolerance towards heavy metal exposure, drought stress and salt stress because it allows the maintenance of high AsA levels [18,54,55,56]. In the present work, the DCPTA pre-treatment resulted in significant increases in MDHAR and DHAR activities and MDHAR1 and MDHAR4 transcript levels, which may be primary causes of the maintenance of lower levels of DHA and higher levels of AsA as well as higher AsA/DHA ratios (compared to drought stress alone) [57,58].…”

Section: Discussionmentioning

confidence: 99%

“…Specific primers for each gene were designed from the 3’ ends of the gene sequences (Table 1). The synthesis of cDNA and real-time PCR were performed as previously described of Liu et al (2012) [18]. The 2 −ΔΔCt method was used to calculate the relative transcript levels.…”

Section: Methodsmentioning

confidence: 99%

“…In maize, multigene families encode the AsA-GSH cycle enzymes. To date, seven APX genes (cytosolic APX1, cytosolic APX2, cytosolic APX4, peroxisomal APX3, mitochondrial APX5, mitochondrial APX6 and chloroplastic APX7), two GR genes (Cytosol GR1 and Cytosol GR2), three DHAR genes (Chloroplast DHAR1, Cytosol DHAR2 and Mitochondria DHAR3) and four MDHAR genes (Cytosol MDAR1, Cytosol MDAR2 and Cytosol MDAR3 and Mitochondrial MDAR4) have been identified [18]. Until now, it has remained unclear how the expression of the genes encoding the enzymes of the AsA-GSH cycle in maize responds to plant growth regulators.…”

Section: Introductionmentioning

confidence: 99%

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Modulating the antioxidant system by exogenous 2-(3,4-dichlorophenoxy) triethylamine in maize seedlings exposed to polyethylene glycol-simulated drought stress

Xie

Zhang

et al. 2018

PLoS ONE

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Maize (Zea mays L.), an important agricultural crop, suffers from drought stress frequently during its growth period, thus leading to a decline in yield. 2-(3,4-Dichlorophenoxy) triethylamine (DCPTA) regulates many aspects of plant development; however, its effects on crop stress tolerance are poorly understood. We pre-treated maize seedlings by adding DCPTA to a hydroponic solution and then subjected the seedlings to a drought condition [15% polyethylene glycol (PEG)-6000 treatment]. The activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR) were enhanced under drought stress and further enhanced by the DCPTA application. The activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and catalase (CAT) declined continuously under drought stress; however, the activities partially recovered with DCPTA application. Up-regulation of the activities and transcript levels of APX, GR, MDHAR and DHAR in the DCPTA treatments contributed to the increases in ascorbate (AsA) and glutathione (GSH) levels and inhibited the increased generation rate of superoxide anion radicals (O2·−), the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA), and the electrolyte leakage (EL) induced by drought. These results suggest that the enhanced antioxidant capacity induced by DCPTA application may represent an efficient mechanism for increasing the drought stress tolerance of maize seedlings.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modulating the antioxidant system by exogenous 2-(3,4-dichlorophenoxy) triethylamine in maize seedlings exposed to polyethylene glycol-simulated drought stress

Xie

Zhang

et al. 2018

PLoS ONE

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“…The critical role of APX and MDAR in stress tolerance (Liu et al 2012) and upregulation of mdar and apx transcripts have been reported by Secenji et al (2008) in tolerant and sensitive varieties of Triticum aestivum under drought stress. Variations in physiological parameters potentially contribute to the complex phenotype of drought tolerance; however, a clear relation between stress and coexpression of antioxidant genes has so far not been evident.…”

Section: Discussionmentioning

confidence: 66%

Drought stress-induced enzyme activity and mdar and apx gene expression in tolerant and susceptible genotypes of Eleusine coracana (L.)

Bartwal

Arora

2016

In Vitro Cell.Dev.Biol.-Plant

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Drought is a major constraint limiting agricultural productivity worldwide. Exposure to drought results in altered physiology of plants and causes oxidative stress. The aim of this study was to evaluate genotypic differences in oxidative stress markers and transcript levels of genes encoding ascorbate peroxidase (APX) and monodehydroascorbate reductase (MDAR) from two finger millet (Eleusine coracana L.) varieties differing in their stress tolerance potential. Seedlings of PR202, a stress-tolerant variety, and PES400, a susceptible variety, were subjected to different polyethylene glycol 6000 (PEG-6000) concentrations (0, 5, 10, 15, and 20%). Increases in activity of APX and MDAR were observed under stress, with maximal increases being recorded at 15 and 20% PEG-6000. At these PEG-6000 levels, PES400 showed comparatively higher accumulations of malondialdehyde and hydrogen peroxide and a lower APX:MDAR ratio than PR202. Adaptive stress response was better in PR202 than in PES400, demonstrating the stress-tolerant character of PR202. Under oxidative stress, the expression of genes encoding APX and MDAR was positively correlated with the respective enzyme activities. The results demonstrated that 15 and 20% PEG-6000 concentrations are optimum for revealing differences in stress response between PR202 and PES400. The results of this study will be useful in understanding the expression of drought-responsive genes in E. coracana.

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Chloroplastic ascorbate peroxidases targeted to stroma or thylakoid membrane: The chicken or egg dilemma

et al. 2022

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Ascorbate peroxidases (APXs) are heme peroxidases that remove hydrogen peroxide in different subcellular compartments with concomitant ascorbate cycling. Here, we analysed and discussed phylogenetic and molecular features of the APX family. Ancient APX originated as a soluble stromal enzyme, and early during plant evolution, acquired both chloroplast‐targeting and mitochondrion‐targeting sequences and an alternative splicing mechanism whereby it could be expressed as a soluble or thylakoid membrane‐bound enzyme. Later, independent duplication and neofunctionalization events in some angiosperm groups resulted in individual genes encoding stromal, thylakoidal and mitochondrial isoforms. These data reaffirm the complexity of plant antioxidant defenses that allow diverse plant species to acquire new means to adapt to changing environmental conditions.

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Gene families of maize glutathione–ascorbate redox cycle respond differently to abiotic stresses

Cited by 50 publications

References 43 publications

Modulating the antioxidant system by exogenous 2-(3,4-dichlorophenoxy) triethylamine in maize seedlings exposed to polyethylene glycol-simulated drought stress

Modulating the antioxidant system by exogenous 2-(3,4-dichlorophenoxy) triethylamine in maize seedlings exposed to polyethylene glycol-simulated drought stress

Drought stress-induced enzyme activity and mdar and apx gene expression in tolerant and susceptible genotypes of Eleusine coracana (L.)

Chloroplastic ascorbate peroxidases targeted to stroma or thylakoid membrane: The chicken or egg dilemma

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