Gene expression studies of Saccharum spontaneum, a wild relative of sugarcane in response to salinity stress

Abstract

The study is aimed to assess the morphological, physiological, and molecular responses of seven Saccharum spontaneum clones for salinity stress. These clones (IND‐07‐1462, IND‐07‐1465, IND‐07‐1470, IND‐07‐1471, IND 16–1761, IND 16–1762, and IND 16–1763) were subjected to salinity stress at two different concentrations of electrical conductivity 6 and 8 ds/m after 60 days of planting. All seven genotypes showed a decrease in relative water content and nitrate reductase activity with an increase in severity of salt stress. The effect was more pronounced in IND‐07‐1471, while IND‐16‐1762 exhibited only a minimum drop. Similarly we observed an increase in proline content and lipid peroxidation activity for the genotype IND‐07‐1471, while IND‐16‐1762 showed minimum increase. Molecular profiling of genes/transcription factors like salt overly sensitive, responsive to abscissic acid, dirigent, myeloblastosis, ethylene responsive factor associated with salinity stress tolerance showed 19‐, 18‐, 17‐, 10‐, and 9‐fold increased expression at 8 ds/m of salinity stress, respectively, in IND‐16‐1762 showed. Based on the evidences obtained from expression profiling, we have cloned the conserved regions of RAB and SOS1 genes. The domain of SOS and RAB was identified as a regulatory subunit of cAMP‐dependent protein kinases which is involved in a signaling pathway.

Deep sequencing of suppression subtractive library identifies differentially expressed transcripts of Saccharum spontaneum exposed to salinity stress

“…Manjunatha et al (2018) studied the variability in 304 S. spontaneum accessions from North eastern India and observed that the accessions showed high variability for both quantitative and qualitative traits. Considering the morphological data and our earlier report (Kasirajan et al, 2021), where IND‐16‐1762 showed an increased expression of 9–19 fold for genes and TFs associated with several stress responses (including salinity stress tolerance), we chose IND‐16‐1762 for the SSH experiment.…”

“…Fourteen different clones were grown under normal environmental conditions in pots and screened at the ICAR‐Sugarcane Breeding Institute (Coimbatore, India) as previously reported in Kasirajan et al (2021). About 90–100 days after planting, the clones were subjected to salinity stress with a saline solution mixture composed of sodium sulfate, sodium chloride, and calcium chloride (Himedia see Table 1 for exact composition), along with Hoagland solution.…”

“…studies(Kasirajan et al, 2021). The secondary PCR products obtained from subtracted and unsubtracted cDNAs were approximately 600 bp in length (Figure1A-D).…”

The Wild Sugarcane and Sorghum Kinomes: Insights Into Expansion, Diversification, and Expression Patterns

“…Additionally, AGC_PKA-PKG subfamily has been reported as broadly important in both Ssp and Sbi. In Ssp studies have demonstrated the association of its members with signaling pathways ( Kasirajan et al, 2020 ), cell proliferation ( Li et al, 2016b ), infection responses ( Santa Brigida et al, 2016 ; Xu et al, 2018 ), hormone signal transduction in response to drought ( Li et al, 2016a ), and pathways related to sucrose storage and photosynthesis ( Hoang et al, 2017 ; Thirugnanasambandam et al, 2017 ). In Sbi, the importance of this family is also linked with stress and signal responses ( Li et al, 2018 ; Parra-Londono et al, 2018 ; Nagaraju et al, 2020 ; Vikal et al, 2020 ).…”

De-novo Domestication for Improving Salt Tolerance in Crops

“…Wild relatives of other important crops also provide the salinity tolerant genes including SOS and RAB in wild sugarcane (Kasirajan et al, 2020 ), ZmHKT2 in wild maize (Cao et al, 2019 ), and HAI1, HAB2 (Liu et al, 2020b ), IpDHN (Zhang et al, 2018a ), and IpSR (Zhang et al, 2018b ) in wild potato ( Ipomoea pes-caprae ).…”