Gene expression studies of developing bovine longissimusmuscle from two different beef cattle breeds

Lehnert, S. A.; Reverter, Antônio; Byrne, Keren; Wang, Yonghong; Nattrass, G. S.; Hudson, Nicholas J.; Greenwood, P. L.

doi:10.1186/1471-213x-7-95

Cited by 117 publications

(103 citation statements)

References 43 publications

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“…Details relating to the animal experiment, RNA extraction, microarray hybridisation and data normalisation can be found in the studies by Wang et al (2005 and, Lehnert et al (2007), Hudson et al (2009a and2009b). As previously described in a study by Lehnert et al (2007), Hereford cows were artificially inseminated or mated to one of five different Wagyu sires (W × H) or one of six different Piedmontese sires (P × H).…”

Section: Methodsmentioning

confidence: 99%

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Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

Hudson

Reverter

Greenwood

et al. 2015

Animal

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Intramuscular fat (IMF) can improve meat product quality through its impact on flavour and juiciness. High marbling cuts can command premium prices in some countries and grading systems, but there is substantial cost involved in choosing to grain feed animals in an effort to deposit more IMF. There would be value in developing methods to predict predisposition to 'marble' well. Unfortunately, the biological mechanisms underpinning marbling remain a mystery: the key adipocyte cell populations have not been defined, there are no reliable DNA markers, no known (if any) causal mutations and gene expression analyses in the main have tended to characterise increases in expression of end-point fat metabolism proteins such as the fatty acid-binding proteins. To shed light on expression-based markers of marbling potential, we contrasted LD gene expression in high IMF Wagyu cross animals with a low IMF Piedmontese cross at various time points. The expected divergence in the fat metabolism genes FABP4, THRSP, CIDEC and ACACA between the breeds occurs surprisingly late in postnatal development at about 20 months. On the other hand, divergent expression of WISP2, RAI14 and CYP4F2 was discovered in animals at or before 12 months of age, suggesting these genes may have potential as earlier predictors of marbling potential. In line with other researchers, we found intriguing links between IMF development and connective tissue remodelling. WISP2 -a novel adipokine highly expressed and secreted by adipose precursor cells and an inhibitor of the pro-fibrotic connective tissue growth factor -emerges as a particularly attractive candidate. It is relatively upregulated in high marbling Wagyu before admission to feedlotting, somewhere between 7 and 12 months. This difference is subsequently maintained until 25 months, but not thereafter. RAI14, thought to play a role in porcine adipocyte differentiation and with links to retinoic acid metabolism, has an unusual expression profile. Its expression level increases monotonically with postnatal development, and is always higher in Wagyu than Piedmontese. Strong, sustained upregulation of the anti-inflammatory CYP4F2 in Piedmontese is consistent with Wagyu adiposity being a pro-inflammatory state. Application of regulatory impact factor analysis, a network method for identifying causal effector molecules, suggests marbling roles for transcription factors previously implicated in (1) the formation of liposarcoma (unconstrained fatty masses) (YEATS4, MDM2), (2) adipogenesis (CREBL2, SP1, STAT1) and (3) inflammation (ISGF3G, HOXB13, PML).

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Section: Methodsmentioning

confidence: 99%

“…As previously described in a study by Lehnert et al (2007), Hereford cows were artificially inseminated or mated to one of five different Wagyu sires (W × H) or one of six different Piedmontese sires (P × H). All Piedmontese sires were homozygous for the MSTN mutation in exon 3.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

Hudson

Reverter

Greenwood

et al. 2015

Animal

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“…Differences in the gene expression in muscle from cattle breeds or porcine breeds with different fat deposition capacity have been reported (Lehnert et al, 2007;Liu and Gao, 2010). Guo et al (2009) found that the inhibition of MSTN signalling in skeletal muscle resulted in increased lean mass, reduced fat mass and improved glucose metabolism.…”

Section: Discussionmentioning

confidence: 97%

Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle

Zheng¹,

Lin²,

Yu³

et al. 2011

CZECH J ANIM SCI

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ABSTRACT:The main objective of this study was to reveal the expression profiles of two negative regulators, myostatin (MSTN) and calpastatin (CAST) genes, of skeletal muscle growth in highland yaks (Bos grunniens). mRNA levels of both genes were quantified in different yak tissues by semi-quantitative RT-PCR to reveal the tissue expression pattern, and real-time quantitative RT-PCR was employed to compare the mRNA levels of MSTN and CAST in longissimus muscles of yaks at different ages and adult Yellow cattle. Intramuscular fat (IMF) content, tenderness and pH of longissimus muscle of yaks at different ages and of adult Yellow cattle were also measured. The results showed that MSTN and CAST expressions have tissue specificity and both exhibited a high level in longissimus muscle and a low level in adipose tissue. Yak calves had lower mRNA levels of both MSTN and CAST in longissimus muscle compared with adult yaks. The analysis of meat quality traits of longissimus muscle showed that the shear forces of raw longissimus muscle of yak calves were significantly lower than those of adult yaks and Yellow cattle, no significant difference was found between adult yaks and Yellow cattle of similar age. IMF content in longissimus muscle was lower in yaks than in Yellow cattle. Although yaks were smaller in body size than Yellow cattle, adult yaks showed lower levels of MSTN and similar level of CAST mRNA in longissimus muscle compared to Yellow cattle. These data indicate that the expression of both MSTN and CAST in longissimus muscle differs between adult yaks and yak calves, and the yak longissimus muscle shows a lower IMF content compared to cattle.

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“…Other studies focussed on fetal muscle development (Sudre et al 2003;Lehnert et al 2007), muscle growth potential (Sudre et al 2005;Cassar-Malek et al 2007) and effects of nutritional changes , which all influence the composition of muscle tissue. Intramuscular fat development was also examined Lee et al 2007) since it influences marbling and thus juiciness and flavour.…”

Section: Application To Meat Qualitymentioning

confidence: 99%

Application of gene expression studies in livestock production systems: a European perspective

Cassar-Malek

Picard

Bernard

et al. 2008

Aust. J. Exp. Agric.

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Abstract. In the context of sustainable agriculture and animal husbandry, understanding animal physiology remains a major challenge in the breeding and production of livestock, especially to develop animal farming systems that respond to the new and diversified consumer demand. Physiological processes depend on the expression of many genes acting in concert. Considerable effort has been expended in recent years on examining the mechanisms controlling gene expression and their regulation by biological and external factors (e.g. genetic determinants, nutritional factors, and animal management). Two main strategies have been developed to identify important genes. The first one has focussed on the expression of candidate genes for key physiological pathways at the level of both the transcripts and proteins. An original strategy has emerged with the advent of genomics that addresses the same issues through the examination of the molecular signatures of all genes and proteins using high-throughput techniques (e.g. transcriptomics and proteomics). In this review, the application of the gene expression studies in livestock production systems is discussed. Some practical examples of genomics applied to livestock production systems (e.g. to optimise animal nutrition, meat quality or animal management) are presented, and their outcomes are considered. In the future, integration of the knowledge gained from these studies will finally result in optimising livestock production systems through detection of desirable animals and their integration into accurate breeding programs or innovative management systems.

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Gene expression studies of developing bovine longissimusmuscle from two different beef cattle breeds

Cited by 117 publications

References 43 publications

Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle

Application of gene expression studies in livestock production systems: a European perspective

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