Gene expression signature induced by grape intake in healthy subjects reveals wide-spread beneficial effects on peripheral blood mononuclear cells

Milella, Rosa Anna; Gasparro, Marica; Alagna, Fiammetta; Cardone, Maria Francesca; Rotunno, Silvia; Ammollo, Concetta T.; Semeraro, Fabrizio; Tullo, Apollonia; Marzano, Flaviana; Catalano, Domenico; Antonacci, Donato; Colucci, Mario; D’Elia, Domenica

doi:10.1016/j.jff.2019.103705

Cited by 8 publications

(6 citation statements)

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“…For this reason, "functional foods" containing components that can protect our health are becoming very popular. Grape is qualitatively and quantitatively rich in bioactive compounds such as polyphenols, and as suggested by our previous studies, the intake of fresh table grapes induces health benefits (Ammollo et al, 2017;Milella et al, 2020;Tutino et al, 2021).…”

Section: Discussionmentioning

confidence: 67%

Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.)

et al. 2023

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Grapes represent a significant source of phenolic compounds known for their health-promoting properties, such as antioxidant capacity on normal cells and prooxidant activity on tumor cells. The genotype highly affects the polyphenolic composition in grapes and, consequently, the nutritional quality of berries. This work aimed to characterize the phenolic composition, the antioxidant, and anticancer activity of grape skin extracts (GSEs) of nine new table grape genotypes selected from a breeding program to obtain new cultivars of seedless table grapes, well adapted to the climatic change and with higher nutraceutical properties. The grape polyphenolic profile was characterized by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry analysis. GSE antioxidant activity was determined by the ABTS, DPPH, and ORAC assays; GSE cell growth inhibition test was carried out in the Caco2 human cancer cell line. The nine GSEs showed different flavonoid and non-flavonoid profiles, and all possessed antioxidant activity, with the ‘Aika N.’, ‘Turese N.’, and ‘Egnatia N.’ the most active. As anticancer activity against the tested cancer cell line, ‘Daunia N.’ and ‘Apenestae N.’ showed the EC50 after 24 h of 35.60 µg/mL and 150.91 µg/mL, respectively. The relationship between polyphenolic profile and the antioxidant and anticancer activity of GSE was also investigated. Interestingly, among the different classes of polyphenolics, flavan-3-ols e proanthocyanidins showed the highest positive correlation with the anticancer activity of extracts. These findings can be helpful for the preparation of new extracts for the pharmaceutical and nutraceutical industry and geneticists working in vine breeding programs.

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Section: Discussionmentioning

confidence: 67%

Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.)

et al. 2023

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“…A previous study showed that table grape extracts exert a marked antithrombotic activity in vitro (Ammollo et al, 2017). To shed light on the molecular basis of grape intake effects on human health and investigate the potential role of ncRNAs, we carried out a nutrigenomics study (Milella et al, 2020a and2020b). To this aim, 20 healthy subjects were enrolled to follow a grape-rich diet for 21 days.…”

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confidence: 99%

Benefits of a grape-rich diet on human health: a nutrigenomics study underlining the potential role of non-coding RNAs

et al. 2021

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“…In the pilot study, gene expression profiling of peripheral blood mononuclear cells (PBMCs) identified 930 differentially expressed transcripts. Microarray experiments showed that after 21 days of a fresh table grape-rich diet, 463 genes are modulated while, one month after the end of the grape-rich diet, this number almost doubled, reaching 849 genes (Table 1) [16,18]. The changes in gene expression more pronounced after the washout period could be explained as a consequence of an indirect effect of grape, e.g.…”

Section: Gene Expression Studymentioning

confidence: 99%

“…In this note, we summarise two separate trials in which healthy subjects consumed the black seedless grape Autumn royal for three consecutive weeks, limiting the intake of other polyphenols. The first work evaluated changes in the gene expression profile of the total transcriptome [16], and the second investigated the influence of table grape intake on the circulating miRNA profile [17].…”

Section: Introductionmentioning

confidence: 99%

Nutrigenomics to reveal the effects of grape consumption in healthy subjects

Anna Milella,

Gasparro,

Giannandrea

et al. 2023

BIO Web Conf.

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The Mediterranean diet places fruit and vegetables as the basis of daily nutrition. Table grape is a typical fruit of the Mediterranean tradition and is consumed worldwide. The CREA Research Centre for Viticulture and Enology has conducted nutrigenomics studies in recent years to investigate the effects of table grapes on human health. This note summarises two trials in which healthy subjects ate the black seedless grape Autumn Royal for three consecutive weeks. For our first nutrigenomic study, we used the microarray technique to analyze thousands of genes' expressions simultaneously. The results showed that 463 genes were modulated, and one month after the end of the grape-rich diet, this number almost doubled, reaching 849 genes. Furthermore, more than 200 of these genes are non-coding RNAs important in regulating gene expression. The second nutrigenomic study was conducted to evaluate the effects of grape intake on the expression of microRNAs, identifying 20 circulating microRNAs modulated, most of which were implicated in cancer development. Our results showed that grape intake exerts beneficial effects by modulating genes involved in critical physiological processes such as the immune response, inflammation, autophagy, DNA repair, and mitochondrial functionality.

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Gene expression signature induced by grape intake in healthy subjects reveals wide-spread beneficial effects on peripheral blood mononuclear cells

Cited by 8 publications

References 50 publications

Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.)

Correlation between antioxidant and anticancer activity and phenolic profile of new Apulian table grape genotypes (V. Vinifera L.)

Benefits of a grape-rich diet on human health: a nutrigenomics study underlining the potential role of non-coding RNAs

Nutrigenomics to reveal the effects of grape consumption in healthy subjects

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