Gene expression responses to Rice tungro spherical virus in susceptible and resistant near-isogenic rice plants

Suppression of cell wall-related genes associated with stunting of Oryza glaberrima infected with Rice tungro spherical virus

“…Accumulation of RTSV in O. sativa TW16 was not detected by ELISA. However, as in the previous studies by Encabo et al (2009) and Satoh et al (2013) showing detection of a very low level of RTSV in TW16 by RT-PCR, we assumed that most O. sativa TW16 plants inoculated with RTSV were infected since most O. sativa TN1 plants (susceptible control) inoculated at the same time were found to be infected with RTSV. The comparison between the height reduction rates (Figure 1, Supplementary Material 2) and the RTSV accumulation (Figure 2, Supplementary Material 3) indicates that the level of RTSV accumulation in plants is not correlated with the severity of stunting.…”

“…Genes for CESA5 and cellulose synthase A6 (CESA6), CSLA9 and cellulose synthase-like C7 (CSLC7), expansin 1, and α-expansin 15 precursor (expansin 15) were selected based on their expression patterns in O. sativa TN1 infected with RTSV. The previous microarray analysis by Satoh et al (2013) showed that the expression 1) was activated for CESA5 and CSLC7 genes, 2) was suppressed for CSLA9 and expansin 15 genes, and 3) was not significantly changed for CESA6 and expansin 1 genes by RTSV infection between 6 and 15 dpi (Table 3). …”

“…The three accessions were selected since the severity of stunting by RTSV was significantly different from one another at 21 dpi (Figure 1, Supplementary Material 2). A genome-wide gene expression analysis in O. sativa TN1 and TW16 had been performed previously by Satoh et al (2013). Genes for CESA5 and cellulose synthase A6 (CESA6), CSLA9 and cellulose synthase-like C7 (CSLC7), expansin 1, and α-expansin 15 precursor (expansin 15) were selected based on their expression patterns in O. sativa TN1 infected with RTSV.…”

“…Cell wall-related genes investigated for expression in O. glaberrima (Table 1) were selected based on their expression patterns in O. sativa TN1 and TW16 after infection with RTSV (Satoh et al, 2013). Genes orthologous between O. sativa and O. glaberrima were identified by BLAST-Like Alignment Tool (BLAT) (Kent, 2002) using the O. sativa japonica gene sequences (http://rice.plantbiology.msu.edu) as query and the O. glaberrima genome assembly (www.genome.arizona.edu) as the database.…”

“…Genome-wide gene expression analyses in O. sativa plants infected with RDV (Shimizu et al, 2007; Satoh et al, 2011) and RSV (Satoh et al, 2010) revealed that many stress response-related genes were activated, whereas various development-related genes, including genes involved in cell wall synthesis, were suppressed in O. sativa plants infected with RDV and RSV, indicating that stunting of O. sativa plants by RDV and by RSV might be associated with the suppression of various development-related genes, especially cell wall-related genes. The expression of many defense- and development-related genes, including cell wall-related genes was also found to be regulated in O. sativa plants by infection with RTSV (Satoh et al, 2013). However, the consequences from such changes in gene expression by RTSV were uncertain since the O. sativa plants infected with RTSV remained asymptomatic.…”