Gene expression profiling toward the next generation safety control of influenza vaccines and adjuvants in Japan

Momose, Haruka; Kuramitsu, Madoka; Hamaguchi, Isao; Mizukami, Tamio

doi:10.1016/j.vaccine.2018.09.021

Cited by 2 publications

(1 citation statement)

References 64 publications

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“…A more recent approach to study pathogen- or vaccine-induced responses is the measurement of differential gene expression patterns between activated and non-activated immune cells which can be investigated by microarray or more recent next generation sequencing technologies ( 118 ). Measuring the effects of vaccination on gene expression can be used to assess both the safety and immunogenicity of investigational vaccines ( 119 , 120 ). Several studies demonstrate that gene expression is altered after natural influenza infection or vaccination.…”

Section: Methods To Assess Cell-mediated Immunitymentioning

confidence: 99%

The role of cell-mediated immunity against influenza and its implications for vaccine evaluation

et al. 2022

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Influenza vaccines remain the most effective tools to prevent flu and its complications. Trivalent or quadrivalent inactivated influenza vaccines primarily elicit antibodies towards haemagglutinin and neuraminidase. These vaccines fail to induce high protective efficacy, in particular in older adults and immunocompromised individuals and require annual updates to keep up with evolving influenza strains (antigenic drift). Vaccine efficacy declines when there is a mismatch between its content and circulating strains. Current correlates of protection are merely based on serological parameters determined by haemagglutination inhibition or single radial haemolysis assays. However, there is ample evidence showing that these serological correlates of protection can both over- or underestimate the protective efficacy of influenza vaccines. Next-generation universal influenza vaccines that induce cross-reactive cellular immune responses (CD4+ and/or CD8+ T-cell responses) against conserved epitopes may overcome some of the shortcomings of the current inactivated vaccines by eliciting broader protection that lasts for several influenza seasons and potentially enhances pandemic preparedness. Assessment of cellular immune responses in clinical trials that evaluate the immunogenicity of these new generation vaccines is thus of utmost importance. Moreover, studies are needed to examine whether these cross-reactive cellular immune responses can be considered as new or complementary correlates of protection in the evaluation of traditional and next-generation influenza vaccines. An overview of the assays that can be applied to measure cell-mediated immune responses to influenza with their strengths and weaknesses is provided here.

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Section: Methods To Assess Cell-mediated Immunitymentioning

confidence: 99%

The role of cell-mediated immunity against influenza and its implications for vaccine evaluation

et al. 2022

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Changes of urine metabolite profiles are induced by inactivated influenza vaccine inoculations in mice

Kusunoki

Momose

Furuhata

et al. 2019

Sci Rep

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The safety evaluation of vaccines is critical to avoid the development of side effects in humans. To increase the sensitivity of detection for toxicity tests, it is important to capture not only pathological changes but also physiological changes. 1H nuclear magnetic resonance (NMR) spectroscopy analysis of biofluids produces profiles that show characteristic responses to changes in physiological status. In this study, mouse urine metabolomics analysis with 1H NMR was performed using different influenza vaccines of varying toxicity to assess the usefulness of 1H NMR in evaluating vaccine toxicity. Two types of influenza vaccines were used as model vaccines: a toxicity reference vaccine (RE) and a hemagglutinin split vaccine. According to the blood biochemical analyses, the plasma alanine transaminase levels were increased in RE-treated mice. Changes in metabolite levels between mice administered different types of influenza vaccines were observed in the 1H NMR spectra of urine, and a tendency toward dosage-dependent responses for some spectra was observed. Hierarchical clustering analyses and principal component analyses showed that the changes in various urine metabolite levels allowed for the classification of different types of vaccines. Among them, two liver-derived metabolites were shown to largely contribute to the formation of the cluster. These results demonstrate the possibility that urine metabolomics analysis could provide information about vaccine-induced toxicity and physiological changes.

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Gene expression profiling toward the next generation safety control of influenza vaccines and adjuvants in Japan

Cited by 2 publications

References 64 publications

The role of cell-mediated immunity against influenza and its implications for vaccine evaluation

The role of cell-mediated immunity against influenza and its implications for vaccine evaluation

Changes of urine metabolite profiles are induced by inactivated influenza vaccine inoculations in mice

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