Gene expression profiling of rice seedlings in response to glutamine treatment

Kan, Chia-Cheng; Chung, Tsui-Yun; Hsieh, Ming‐Hsiun

doi:10.1016/j.gdata.2015.08.023

Cited by 4 publications

(2 citation statements)

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“…We have previously shown that glutamine can effectively support rice seedling growth when supplemented as the sole nitrogen source in hydroponics [ 33 ]. In addition to its role in plant nutrition, glutamine can rapidly induce the expression of key transcription factor genes involved in nitrogen and stress responses in rice roots [ 33 , 34 ]. These findings support the notion that amino acid signaling pathways may crosstalk with biotic and abiotic signaling networks in plants.…”

Section: Introductionmentioning

confidence: 99%

Exogenous glutamate rapidly induces the expression of genes involved in metabolism and defense responses in rice roots

Kan

Chung

et al. 2017

BMC Genomics

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BackgroundGlutamate is an active amino acid. In addition to protein synthesis and metabolism, increasing evidence indicates that glutamate may also function as a signaling molecule in plants. Still, little is known about the nutritional role of glutamate and genes that are directly regulated by glutamate in rice.ResultsExogenous glutamate could serve as a nitrogen nutrient to support the growth of rice seedlings, but it was not as effective as ammonium nitrate or glutamine. In nitrogen-starved rice seedlings, glutamate was the most abundant free amino acid and feeding of glutamate rapidly and significantly increased the endogenous levels of glutamine, but not glutamate. These results indicated that glutamate was quickly metabolized and converted to the other nitrogen-containing compounds in rice. Transcriptome analysis revealed that at least 122 genes involved in metabolism, transport, signal transduction, and stress responses in the roots were rapidly induced by 2.5 mM glutamate within 30 min. Many of these genes were also up-regulated by glutamine and ammonium nitrate. Still, we were able to identify some transcription factor, kinase/phosphatase, and elicitor-responsive genes that were specifically or preferentially induced by glutamate.ConclusionsGlutamate is a functional amino acid that plays important roles in plant nutrition, metabolism, and signal transduction. The rapid and specific induction of transcription factor, kinase/phosphatase and elicitor-responsive genes suggests that glutamate may efficiently amplify its signal and interact with other signaling pathways to regulate metabolism, growth and defense responses in rice.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3588-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Exogenous glutamate rapidly induces the expression of genes involved in metabolism and defense responses in rice roots

Kan

Chung

et al. 2017

BMC Genomics

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“…When Gln was administered to maize seedlings via tip-cut leaves, root N uptake was shown to decrease [37]. In addition to transporter regulation, increased Gln was shown to control at least 35 general stress response genes in rice [39,40]. Increased Gln has also been implicated in coordinating sulphate and N nutrition in barley [41], and to exert control over the C4 photosynthetic pathway in maize [42].…”

Section: Implications Of a Limited Gln-availability Period In Maize Lmentioning

confidence: 99%

Biosensor-Mediated In Situ Imaging Defines the Availability Period of Assimilatory Glutamine in Maize Seedling Leaves Following Nitrogen Fertilization

Goron¹,

Raizada²

2017

Nitrogen

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Abstract:The amino acid glutamine (Gln) is an important assimilatory intermediate between root-derived inorganic nitrogen (N) (i.e., ammonium) and downstream macromolecules, and is a central regulator in plant N physiology. The timing of Gln accumulation after N uptake by roots has been well characterized. However, the duration of availability of accumulated Gln at a sink tissue has not been well defined. Measuring Gln availability would require temporal measurements of both Gln accumulation and its reciprocal depletion. Furthermore, as Gln varies spatially within a tissue, whole-organ in situ visualization would be valuable. Here, the accumulation and subsequent disappearance of Gln in maize seedling leaves (Zea mays L.) was imaged in situ throughout the 48 h after N application to roots of N-deprived plants. Free Gln was imaged by placing leaves onto agar embedded with bacterial biosensor cells (GlnLux) that emit luminescence in the presence of leaf-derived Gln. Seedling leaves 1, 2, and 3 were imaged simultaneously to measure Gln availability across tissues that potentially vary in N sink strength. The results show that following root N fertilization, free Gln accumulates and then disappears with an availability period of up to 24 h following peak accumulation. The availability period of Gln was similar in all seedling leaves, but the amount of accumulation was leaf specific. As Gln is not only a metabolic intermediate, but also a signaling molecule, the potential importance of regulating its temporal availability within plant tissues is discussed.

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Influence of N nutrients on GS activity and putative ammonium transporter1;2 (SaAMT1;2) expression in sandal plants (Santalum album L.)

Yusuf

Deepa

2017

Trees

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Gene expression profiling of rice seedlings in response to glutamine treatment

Cited by 4 publications

References 4 publications

Exogenous glutamate rapidly induces the expression of genes involved in metabolism and defense responses in rice roots

Exogenous glutamate rapidly induces the expression of genes involved in metabolism and defense responses in rice roots

Biosensor-Mediated In Situ Imaging Defines the Availability Period of Assimilatory Glutamine in Maize Seedling Leaves Following Nitrogen Fertilization

Influence of N nutrients on GS activity and putative ammonium transporter1;2 (SaAMT1;2) expression in sandal plants (Santalum album L.)

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