Gene expression profiling of resistant and susceptible Bombyx mori strains reveals nucleopolyhedrovirus-associated variations in host gene transcript levels

Bao, Yan-Yuan; Tang, Xudong; Lv, Zu-Yao; Wang, Xiuye; Tian, Cai-Hong; Xu, Yi; Zhang, Chuan‐Xi

doi:10.1016/j.ygeno.2009.04.003

Cited by 97 publications

(73 citation statements)

References 23 publications

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“…Iwanaga et al (21) reported the upregulation of seven genes and downregulation of four genes in the early phase of infection by subtractive hybridization and Northern blot analysis. Using suppression subtractive hybridization (SSH), Bao et al (4,5) reported that a total of 100 genes might be involved in the host antiviral mechanism. Specifically, a total of 15 viral proteins show similarity to silkworm proteins, which are categorized into the following groups: DNA polymerases, protein kinases, inhibitors of apoptosis, ubiquitin, chitinase, ecdysteroid UDP-glucosyltransferase, superoxide dismutase, fibroblast growth factor, SNF2 homolog, desmoplakin, methyltransferase, cathepsin, and protein tyrosine phosphatase (26).…”

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confidence: 99%

Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Xue

Qiao

Zhang

et al. 2012

J Virol

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Although microarray and expressed sequence tag (EST)-based approaches have been used to profile gene expression during baculovirus infection, the response of host genes to baculovirus infection and the interaction between baculovirus and its host remain largely unknown. To determine the host response to Bombyx mori nucleopolyhedrovirus infection and the dynamic interaction between the virus and its host, eight digital gene expression libraries were examined in a Bm5 cell line before infection and at 1.5, 3, 6, 12, 24, 48, and 96 h postinfection. Gene set enrichment analysis of differentially expressed genes at each time point following infection showed that gene sets including cytoskeleton, transcription, translation, energy metabolism, iron ion metabolism, and the ubiquitin-proteasome pathway were altered after viral infection. In addition, a time course depicting protein-protein interaction networks between the baculovirus and the host were constructed and revealed that viral proteins interact with a multitude of cellular machineries, such as the proteasome, cytoskeleton, and spliceosome. Several viral proteins, including IE2, CG30, PE38, and PK-1/2, were predicted to play key roles in mediating virus-host interactions. Based on these results, we tested the role of the ubiquitin-proteasome pathway and iron ion metabolism in the viral infection cycle. Treatment with a proteasome inhibitor and deferoxamine mesylate in vitro and in vivo confirmed that these pathways regulate viral infection. Taken together, these findings provide new insights into the interaction between the baculovirus and its host and identify molecular mechanisms that can be used to block viral infection and improve baculovirus expression systems.

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confidence: 99%

Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Xue

Qiao

Zhang

et al. 2012

J Virol

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“…In the present study, BmNPV infection caused a strong induction of B. mori gloverin gene expression in larval midguts at 24 hpi. The regulatory factors activated or blocked by BmNPV invasion, which caused a differential induction of antibacterial peptide genes in the B. mori susceptible and resistant races (Bao et al, 2009). In resistant larval midguts, these genes promptly responded to BmNPV infection at an early stage (24 hpi) indicating that they might act with each other and develop an effective defense mechanism against BmNPV infection.…”

Section: Analysis Of Bmnpv Proliferation and Differential Level Of Gementioning

confidence: 99%

“…The information available on viral immune response in silkworm Bombyx mori remains scanty, except few publications on Bm lipase, Bm serine protease and few immune related proteins. (Ponnuvel et al, 2003;Nakazawa et al, 2004;Bao et al, 2009).…”

Section: Rna Isolation and First Strand Cdna Synthesismentioning

confidence: 99%

“…(Bao et al, 2009) selected 6 hpi as a time point, to determine the resistance-related genes in the B.mori midgut, using suppressive subtractive hybridization, and found significant up regulation of eight genes from two subtracted cDNA libraries. Indian silkworm strains are known to have resistant character to viral pathogens.…”

Section: Rna Isolation and First Strand Cdna Synthesismentioning

confidence: 99%

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Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

Lekha¹,

Vijayagowri²,

Sasibhushan³

et al. 2014

International Journal of Industrial Entomology

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The variation in the level of immune response related gene expression in silkworm, Bombyx mori following infection with Bombyx mori nucleopolyhedrovirus (BmNPV) was analyzed at different time intervals. The occlusion bodies of BmNPV orally inoculated to the two most divergent silkworm races viz., Sarupat (resistant to BmNPV infection) and CSR2 (susceptible to BmNPV infection) were subjected to oral BmNPV inoculation. The expression profile of gp41 gene of BmNPV in the Sarupat and CSR2 races revealed that the virus could invade the midguts of both susceptible and resistant races. However, its multiplication was significantly less in the midgut of resistant race, while, in the susceptible race, the viral multiplication reached maximum level within 12 h. These findings indicate that potential host genes are involved in the inhibition of viral multiplication within larval midgut.

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“…Our results provide new evidence for this relationship. We showed that genes induced during NPV infection (35,36) were also induced in the hemocytes and fat body cells of the silkworm when PP was activated. These findings suggest that various molecules involved in the defense system against NPV infection may be induced by PP activation.…”

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confidence: 96%

Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (Bombyx mori) muscle contraction assay

et al. 2012

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In silkworm larvae, the mature form of paralytic peptide (PP), an insect cytokine, is produced from pro-PP in association with activation of innate immune responses, resulting in slow muscle contraction. We utilized this reaction, muscle contraction in silkworms coupled with innate immunity stimulation, to quantitatively measure the innate immune stimulating activity of various natural polysaccharides. β-Glucan of Gyrophora esculenta (GE-3), fucoidan from sporophyll of Undaria pinnatifida, and curldan induced silkworm muscle contraction. We further demonstrated that GE-3 had therapeutic effects on silkworms infected by baculovirus. Based on these findings, we propose that the silkworm muscle contraction assay is useful for screening substances that stimulate innate immunity before evaluating therapeutic effectiveness in mammals.

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Gene expression profiling of resistant and susceptible Bombyx mori strains reveals nucleopolyhedrovirus-associated variations in host gene transcript levels

Cited by 97 publications

References 23 publications

Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Differential Level of Host Gene Expression Associated with Nucleopolyhedrovirus Infection in Silkworm Races of Bombyx mori

Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (Bombyx mori) muscle contraction assay

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