Gene Expression Profiling of Preovulatory Follicle in the Buffalo Cow: Effects of Increased IGF-I Concentration on Periovulatory Events

Rao, Jyotsna U.; Shah, Kunal B; Puttaiah, Jayaram; Medhamurthy, R.

doi:10.1371/journal.pone.0020754

Cited by 30 publications

(21 citation statements)

References 121 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In agreement with these findings, knockdown of miR-21 in mouse granulosa cells resulted in a transient increase in PTEN protein levels (Carletti et al 2010). A decrease in the expression of PTEN and RASA1 during ovulation is consistent with previous follicular data on cattle (Rao et al 2011) and with the involvement of these two gene products in negative regulation of AKT and RAS, two cell signaling pathways involved in survival and proliferation/differentiation, and which activation in granulosa cells is central to LH-induced oocyte maturation, ovulation, and luteinization (Gilbert et al 2011, Fan et al 2012. In contrast to PTEN and RASA1, we did not detect any differences in transcript levels of the antiproliferative factor, BTG2, between DO and L follicles.…”

Section: Mirnas In Equine Folliclessupporting

confidence: 91%

Involvement of miRNAs in equine follicle development

et al. 2013

View full text Add to dashboard Cite

Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian follicle development. The objective of this study was to determine changes in the levels of these miRNAs in relation to follicle selection, maturation, and ovulation in the monovular equine ovary. In Experiment 1, follicular fluid was aspirated during ovulatory cycles from the dominant (DO) and largest subordinate (S) follicles of an ovulatory wave and the dominant (DA) follicle of a mid-cycle anovulatory wave (nZ6 mares). Follicular fluid levels of progesterone and estradiol were lower (P!0.01) in S follicles than in DO follicles, whereas mean levels of IGF1 were lower (P!0.01) in S and DA follicles than in DO follicles. Relative to DO and DA follicles, S follicles had higher (P%0.01) follicular fluid levels of miR-145 and miR-378. In Experiment 2, follicular fluid and granulosa cells were aspirated from dominant follicles before (DO) and 24 h after (L) administration of an ovulatory dose of hCG (nZ5 mares/group). Relative to DO follicles, L follicles had higher follicular fluid levels of progesterone (PZ0.05) and lower granulosa cell levels of CYP19A1 and LHCGR (P!0.005). Levels of miR-21, miR-132, miR-212, and miR-224 were increased (P!0.05) in L follicles; this was associated with reduced expression of the putative miRNA targets, PTEN, RASA1, and SMAD4. These novel results may indicate a physiological involvement of miR-21, miR-145, miR-224, miR-378, miR-132, and miR-212 in the regulation of cell survival, steroidogenesis, and differentiation during follicle selection and ovulation in the monovular ovary.

show abstract

Section: Mirnas In Equine Folliclessupporting

confidence: 91%

Involvement of miRNAs in equine follicle development

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Recently, the authors have reported that insulin activates TI cell proliferation and the expression of cell-cycle-regulatory components (CDK4, CCND3, and PCNA) by triggering the mTORC1-dependent pathway (Palaniappan et al 2013). Rao et al (2011) analyzed changes in transcriptome of preovulatory follicles in response to the LH surge in buffalo cows. The global gene expression data suggested differential expression of many genes associated with the IGF1 system and its signaling (Table 2).…”

Section: Pi3k/pten/akt and Tsc/mtor Pathways And Ovarian Somatic Cellsmentioning

confidence: 99%

PI3K/PTEN/Akt and TSC/mTOR signaling pathways, ovarian dysfunction, and infertility: an update

Makker

Goel

Mahdi

2014

Journal of Molecular Endocrinology

177

115

View full text Add to dashboard Cite

Abnormalities in ovarian function, including defective oogenesis and folliculogenesis, represent a key female reproductive deficiency. Accumulating evidence in the literature has shown that the PI3K/PTEN/Akt and TSC/mTOR signaling pathways are critical regulators of ovarian function including quiescence, activation, and survival of primordial follicles, granulosa cell proliferation and differentiation, and meiotic maturation of oocytes. Dysregulation of these signaling pathways may contribute to infertility caused by impaired follicular development, intrafollicular oocyte development, and ovulation. This article reviews the current state of knowledge of the functional role of the PI3K/PTEN/Akt and TSC/mTOR pathways during mammalian oogenesis and folliculogenesis and their association with female infertility.

show abstract

“…Each of these developmental steps involves sequential and profound changes in tissue structure and function, requiring finely tuned and coordinated changes in the expression of thousands of genes. Transcriptome analyses have identified many such genes during follicle selection, maturation, and the follicle-luteal transition (Gilbert et al 2011, Rao et al 2011, Christenson et al 2013, although relatively little is still known about the molecular mechanisms regulating their expression, particularly at the posttranscriptional level.…”

Section: Introductionmentioning

confidence: 99%

Characterization of microRNAs differentially expressed during bovine follicle development

Sontakke¹,

Mohammed²,

McNeilly³

et al. 2014

Reproduction

View full text Add to dashboard Cite

Several different miRNAs have been proposed to regulate ovarian follicle function; however, very limited information exists on the spatiotemporal patterns of miRNA expression during follicle development. The objective of this study was to identify, using microarray, miRNA profiles associated with growth and regression of dominant-size follicles in the bovine monovular ovary and to characterize their spatiotemporal distribution during development. The follicles were collected from abattoir ovaries and classified as small (4-8 mm) or large (12-17 mm); the latter were further classified as healthy or atretic based on estradiol and CYP19A1 levels. Six pools of small follicles and individual large healthy (nZ6) and large atretic (nZ5) follicles were analyzed using Exiqon's miRCURY LNA microRNA Array 6th gen, followed by qPCR validation. A total of 17 and 57 sequences were differentially expressed (greater than or equal to twofold; P!0.05) between large healthy and each of small and large atretic follicles respectively. Bovine miRNAs confirmed to be upregulated in large healthy follicles relative to small follicles (bta-miR-144, bta-miR-202, bta-miR-451, bta-miR-652, and bta-miR-873) were further characterized. Three of these miRNAs (bta-miR-144, bta-miR-202, and bta-miR-873) were also downregulated in large atretic follicles relative to large healthy follicles. Within the follicle, these miRNAs were predominantly expressed in mural granulosa cells. Further, body-wide screening revealed that bta-miR-202, but not other miRNAs, was expressed exclusively in the gonads. Finally, a total of 1359 predicted targets of the five miRNAs enriched in large healthy follicles were identified, which mapped to signaling pathways involved in follicular cell proliferation, steroidogenesis, prevention of premature luteinization, and oocyte maturation.

show abstract

Gene Expression Profiling of Preovulatory Follicle in the Buffalo Cow: Effects of Increased IGF-I Concentration on Periovulatory Events

Cited by 30 publications

References 121 publications

Involvement of miRNAs in equine follicle development

Involvement of miRNAs in equine follicle development

PI3K/PTEN/Akt and TSC/mTOR signaling pathways, ovarian dysfunction, and infertility: an update

Characterization of microRNAs differentially expressed during bovine follicle development

Contact Info

Product

Resources

About