Gene expression profiling of potato responses to cold, heat, and salt stress

Abstract

In order to identify genes involved in abiotic stress responses in potato, seedlings were grown under controlled conditions and subjected to cold (4 degrees C), heat (35 degrees C), or salt (100 mM NaCl) stress for up to 27 h. Using an approximately 12,000 clone potato cDNA microarray, expression profiles were captured at three time points following initiation of the stress (3, 9, and 27 h) from two different tissues, roots and leaves. A total of 3,314 clones could be identified as significantly up- or down-regulated in response to at least one stress condition. The genes represented by these clones encode transcription factors, signal transduction factors, and heat-shock proteins which have been associated with abiotic stress responses in Arabidopsis and rice, suggesting similar response pathways function in potato. These stress-regulated clones could be separated into either stress-specific or shared-response clones, suggesting the existence of general response pathways as well as more stress-specific pathways. In addition, we identified expression profiles which are indicative for the type of stress applied to the plants.

Understanding the molecular events underpinning cultivar differences in the physiological performance and heat tolerance of cotton (Gossypium hirsutum)

“…Additionally, induction of genes encoding redox regulating enzymes indicate increased oxidative stress and are consistent with large increases in nonphotochemical quenching (42%) under heat stress (Cottee et al 2012). These trends are consistent with the initial heat stress response of potato (Rensink et al 2005), tobacco (Rizhsky et al 2002), sunflower (Hewezi et al 2008), Festuca sp. (Zhang et al 2005) and A. thaliana (Rizhsky et al 2004;Busch et al 2005;Lim et al 2006;Kant et al 2008), and indicate that plants respond at a molecular level in a similar manner to temperatures well above their thermal optimum.…”

“…Plant responses to high temperature stress at a molecular level have been studied predominantly in model systems such as Arabidopsis thaliana (L.), although some crop species with a higher inherent heat tolerance such as sunflower (Helianthus annuus L.), potato (Solanum tuberosum L.) and tobacco (Nicotiana tabacum L.) have also been studied (Rizhsky et al 2002;Rensink et al 2005;Hewezi et al 2008) and show similar responses to abiotic stress as model species (Rizhsky et al 2004). Molecular analysis indicates that the heat response is complex and varied, involving significant changes in global gene expression (Rizhsky et al 2004;Busch et al 2005;Rensink et al 2005;Kant et al 2008;Larkindale and Vierling 2008) and multigene interactions (Humphreys and Humphreys 2005).…”

“…Molecular analysis indicates that the heat response is complex and varied, involving significant changes in global gene expression (Rizhsky et al 2004;Busch et al 2005;Rensink et al 2005;Kant et al 2008;Larkindale and Vierling 2008) and multigene interactions (Humphreys and Humphreys 2005). The initial heat stress response occurs within the first 0.5-1 h (Busch et al 2005;Kant et al 2008) after exposure to temperatures exceeding the thermal kinetic window that permits normal enzyme function in plants (Burke et al 1988) but may vary between species.…”

Identification of a drought- and cold-stress inducibleWRKYgene in the cold-hardyCitrusrelativePoncirus trifoliata

“…Abiotic stresses in plants induce physiological and biochemical changes controlled by regulation of gene expression. Genome-wide gene-expression studies in cold-acclimated plants have shown that expression of several hundred genes are induced in response to low temperatures in the model plant Arabidopsis thaliana (Seki et al 2001(Seki et al , 2002Fowler & Thomashow 2002;Matsui et al 2010) and economically important crops, such as rice (Rabbani et al 2003), sugarcane (Nogueira et al 2003), pepper (Hwang et al 2005), potato (Rensink et al 2005) and sunflower (Fernandez et al 2008). expression constitute a major group of genes induced in response to abiotic stresses (Seki et al 2001(Seki et al , 2002Fowler & Thomashow 2002;Sakuma et al 2002;S¸ahin-Ç evik & Moore 2006aS¸ahin-Ç evik & Moore , 2006bWei at al.…”

Respuesta transcripcional de Populus a bifenilos policlorados: Aspectos aplicados a la fitorremediación

“…Sin embargo también se ha encontrado su participación en mecanismos de respuesta a estrés abiótico (Alves et al, 2015;. Ejemplos: estrés por sequía (Davey et al, 2009;Golldack et al, 2011;Ranjan et al, 2012;Yan et al, 2012; ELEMENTOS REGULADORES Bhardwaj et al, 2013;Cao et al, 2013); estrés por temperatura, la cual incluye altas temperaturas, bajas temperaturas y congelación (Rensink et al, 2005;Lim et al, 2006;Bedon et al, 2010;Yun et al, 2010;Jiang et al, 2013;Tian et al, 2013); estrés por metales, como el hierro (Colangelo y Guerinot, 2004;Palmer et al, 2013) y el aluminio (Zeng et al, 2012;Yang et al, 2012b); y estrés por luz (Stracke et al, 2010;Rius et al, 2012), entre otros.…”