Gene expression profiling of normal thyroid tissue from patients with thyroid carcinoma

Ria, Roberto; Simeon, Vittorio; Melaccio, Assunta; Meo, Giovanna Di; Trino, Stefania; Mazzoccoli, Carmela; Saltarella, Ilaria; Lamanuzzi, Aurelia; Morano, Annalisa; Gurrado, Angela; Pasculli, Alessandro; Lastilla, Gaetano; Musto, Pellegrino; Reale, Antonia; Dammacco, Franco; Vacca, Angelo; Testini, Mario

doi:10.18632/oncotarget.8820

Cited by 13 publications

(13 citation statements)

References 37 publications

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“…HBEGF has been proved to be critical in tumorigenesis, progression and metastasis of different solid tumors [23][24][25]. Previous studies showed that HBEGF was up-regulated in thyroid cancer [26] and promoted the invasion and metastasis of thyroid cancer cells [26,27]. It was hence speculated that miR-376c-3p might inhibit MTC by downregulating HBEGF.…”

Section: Discussionmentioning

confidence: 99%

MiR-376c-3p targets heparin-binding EGF-like growth factor (HBEGF) to inhibit proliferation and invasion in medullary thyroid carcinoma cells

Bai

Hou

Mao

et al. 2020

aoms

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Introduction: Aggressive medullary thyroid carcinomas (MTC) have a high mortality rate and the treatment for patients diagnosed with advanced MTC is comparatively ineffective. We hence aimed to test the effects of miR-376c-3p on MTC and to explore the relevant mechanism. Material and methods: Cell Counting Kit-8 (CCK-8) and soft agar colony formation assay were applied to evaluate the proliferation of transfected MZ-CRC-1 cells. Wound healing and transwell assay were employed to evaluate MTC cell migration and invasion, respectively. Luciferase assay was performed to validate the downstream target of miR-376c-3p in MZ-CRC-1 cells. Quantitative polymerase chain reaction was used to detect mRNA abundance of key genes. Western blot technique was used to analyze protein levels of HBEGF, E-cadherin, ZO-1, N-cadherin and vimentin. Results: MiR-376c-3p inhibited the viability, migration and invasion of MZ-CRC-1 cells. Moreover, miR-376c-3p mimic downregulated expression of N-cadherin and vimentin but upregulated that of E-cadherin and ZO-1 in MZ-CRC-1 cells. Results for the luciferase reporter assay showed that miR-376c-3p was able to bind the 3′ untranslated region of heparin-binding EGF-like growth factor (HBEGF), of which overexpression nearly nullified the miR-376c-3p mimic-induced inhibitory effects in the MTC cells. Conclusions: MiR-376c-3p showed suppressive effects on MZ-CRC-1 cells via targeting and downregulating HBEGF, suggesting that miR-376c-3p could potentially be targeted for the treatment of MTC.

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Section: Discussionmentioning

confidence: 99%

MiR-376c-3p targets heparin-binding EGF-like growth factor (HBEGF) to inhibit proliferation and invasion in medullary thyroid carcinoma cells

Bai

Hou

Mao

et al. 2020

aoms

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“…To identify such DEGs, microarray gene expression profiling is widely used [13,14], and several such studies have been performed on thyroid cancer subtypes [15]. For example, Huang et al [16] studied gene expression profiles of thyroid cancers, although not functional interactions between gene products.…”

Section: Introductionmentioning

confidence: 99%

Network-Based Genetic Profiling Reveals Cellular Pathway Differences Between Follicular Thyroid Carcinoma and Follicular Thyroid Adenoma

Hossain

Akter

Rahman

et al. 2020

IJERPH

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Molecular mechanisms underlying the pathogenesis and progression of malignant thyroid cancers, such as follicular thyroid carcinomas (FTCs), and how these differ from benign thyroid lesions, are poorly understood. In this study, we employed network-based integrative analyses of FTC and benign follicular thyroid adenoma (FTA) lesion transcriptomes to identify key genes and pathways that differ between them. We first analysed a microarray gene expression dataset (Gene Expression Omnibus GSE82208, n = 52) obtained from FTC and FTA tissues to identify differentially expressed genes (DEGs). Pathway analyses of these DEGs were then performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) resources to identify potentially important pathways, and protein-protein interactions (PPIs) were examined to identify pathway hub genes. Our data analysis identified 598 DEGs, 133 genes with higher and 465 genes with lower expression in FTCs. We identified four significant pathways (one carbon pool by folate, p53 signalling, progesterone-mediated oocyte maturation signalling, and cell cycle pathways) connected to DEGs with high FTC expression; eight pathways were connected to DEGs with lower relative FTC expression. Ten GO groups were significantly connected with FTC-high expression DEGs and 80 with low-FTC expression DEGs. PPI analysis then identified 12 potential hub genes based on degree and betweenness centrality; namely, TOP2A, JUN, EGFR, CDK1, FOS, CDKN3, EZH2, TYMS, PBK, CDH1, UBE2C, and CCNB2. Moreover, transcription factors (TFs) were identified that may underlie gene expression differences observed between FTC and FTA, including FOXC1, GATA2, YY1, FOXL1, E2F1, NFIC, SRF, TFAP2A, HINFP, and CREB1. We also identified microRNA (miRNAs) that may also affect transcript levels of DEGs; these included hsa-mir-335-5p, -26b-5p, -124-3p, -16-5p, -192-5p, -1-3p, -17-5p, -92a-3p, -215-5p, and -20a-5p. Thus, our study identified DEGs, molecular pathways, TFs, and miRNAs that reflect molecular mechanisms that differ between FTC and benign FTA. Given the general similarities of these lesions and common tissue origin, some of these differences may reflect malignant progression potential, and include useful candidate biomarkers for FTC and identifying factors important for FTC pathogenesis.

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“…O carcinoma de tireoide é o mais comum dentre todos os tumores de cabeça e pescoço, sendo o que mais acomete o sistema endócrino (CHA; KOO, 2016;KIRSCHNER et al, 2016). A incidência desse tipo de câncer tem aumentado gradativamente a nível mundial acometendo tanto mulheres quanto homens, entre todas as idades e diferentes grupos étnicos (YIP, 2015;RIA et al, 2016).…”

Section: Introduçâounclassified

Expressão Do Gene Cited1 Em Carcinoma Papilífero De Tireóide: Um Potencial Marcador Diagnóstico

et al. 2019

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O câncer de tireoide é a neoplasia mais comum do sistema endócrino, apresentando incidência crescente nos últimos anos. O diagnóstico é realizado principalmente pela associação de dois métodos: a ultrassonografia e a punção aspirativa por agulha fina (PAAF); porém ambos apresentam uma elevada taxa de resultados indeterminados. Deste modo, é necessária a busca de métodos diagnósticos alternativos para a obtenção de resultados mais precisos e confiáveis. A biologia molecular é uma área que se destaca nesse segmento de pesquisa, buscando compreender a biologia tumoral por meio da avaliação das alterações de expressão gênica. A técnica de PCR quantitativa em tempo real (RT-qPCR) é um método rápido e eficiente de detecção e quantificação de expressão gênica, apresentando alta sensibilidade e especificidade, e sendo considerada como uma ferramenta útil no diagnóstico de vários tipos de câncer. Neste estudo, amostras de carcinomas foliculares de tireoide, carcinomas papilíferos de tireoide, bócio adenomatoso e amostras de tecido normal da glândula foram obtidas com o objetivo de detectar marcadores moleculares diagnósticos por meio da avaliação da expressão dos genes TERT, CITED1 e SLC5A5 pela técnica de RT-qPCR, detectados com expressão aumentada nestas lesões, respectivamente. A expressão diferencial significativa foi detectada somente para o gene CITED1 na comparação das amostras de carcinoma papilífero de tireoide com bócio e tecidos normais, com aumento de expressão deste gene nas amostras de carcinoma papilifero. Os resultados obtidos sugerem o gene CITED1 como um potencial marcador diagnóstico em carcinoma papilífero de tireoide.

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Gene expression profiling of normal thyroid tissue from patients with thyroid carcinoma

Cited by 13 publications

References 37 publications

MiR-376c-3p targets heparin-binding EGF-like growth factor (HBEGF) to inhibit proliferation and invasion in medullary thyroid carcinoma cells

MiR-376c-3p targets heparin-binding EGF-like growth factor (HBEGF) to inhibit proliferation and invasion in medullary thyroid carcinoma cells

Network-Based Genetic Profiling Reveals Cellular Pathway Differences Between Follicular Thyroid Carcinoma and Follicular Thyroid Adenoma

Expressão Do Gene Cited1 Em Carcinoma Papilífero De Tireóide: Um Potencial Marcador Diagnóstico

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