Gene Expression Profiling of Human Kidneys Undergoing Laparoscopic Donor Nephrectomy

Ye, Xu; Jianli,; Ma, Xiaoping; Lü, Yi; Chen, Ping; Luo, Shunwen; Jia, Zhigang; Liu, Yang; Guo, Yu

doi:10.4293/10860813x13693422519154

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…Furthermore, nephrectomy can activate immune cells in the spleen (Lukacs-Kornek et al, 2008 ). In human kidney donors, the activation of cytokine signaling pathways through STATs and SOCS has been shown to occur (Xu et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Uninephrectomy in rats on a fixed food intake results in adipose tissue lipolysis implicating spleen cytokines

et al. 2015

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The role of mild kidney dysfunction in altering lipid metabolism and promoting inflammation was investigated in uninephrectomized rats (UniNX) compared to Sham-operated controls rats. The impact of UniNX was studied 1, 2, and 4 weeks after UniNX under mild food restriction at 90% of ad libitum intake to ensure the same caloric intake in both groups. UniNX resulted in the reduction of fat pad weight. UniNX was associated with increased circulating levels of beta-hydroxybutyrate and glycerol, as well as increased fat pad mRNA of hormone sensitive lipase and adipose triglyceride lipase, suggesting enhanced lipolysis. No decrease in fat pad lipogenesis as assessed by fatty acid synthase activity was observed. Circulating hormones known to regulate lipolysis such as leptin, T3, ghrelin, insulin, corticosterone, angiotensin 1, and angiotensin 2 were not different between the two groups. In contrast, a select group of circulating lipolytic cytokines, including interferon-gamma and granulocyte macrophage–colony stimulating factor, were increased after UniNX. These cytokine levels were elevated in the spleen, but decreased in the kidney, liver, and fat pads. This could be explained by anti-inflammatory factors SIRT1, a member of the sirtuins, and the farnesoid x receptor (FXR), which were decreased in the spleen but elevated in the kidney, liver, and fat pads (inguinal and epididymal). Our study suggests that UniNX induces adipose tissue lipolysis in response to increased levels of a subset of lipolytic cytokines of splenic origin.

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Section: Discussionmentioning

confidence: 99%

Uninephrectomy in rats on a fixed food intake results in adipose tissue lipolysis implicating spleen cytokines

et al. 2015

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“…Luteolin was observed to decrease sorbitol accumulation in the rat lens under high-sorbitol conditions ex vivo via high inhibitory activity against AR and may be used as natural drugs for treating diabetic complications [36]. Luteolin was also identified as the small-molecule drug during identifying the differentially expressed genes including ESR1 in kidneys undergoing laparoscopic donor nephrectomy [37]. The protein expression of GSTP1 was mainly dominated by AhR pathway and luteolin inhibited the expression of drug-metabolizing enzymes by modulating Nrf2 and AhR pathways [38].…”

Section: Resultsmentioning

confidence: 99%

Systematic Investigation of Scutellariae Barbatae Herba for Treating Hepatocellular Carcinoma Based on Network Pharmacology

Gong

Kao

Zhang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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As the fifth most common type of malignant cancers globally, hepatocellular carcinoma (HCC) is the second leading cause of cancer-related mortality worldwide. As a long-time medicinal herb in Traditional Chinese Medicine (TCM), Scutellariae Barbatae Herba (SBH) has also been used for treating various cancers including HCC, but its underlying mechanisms have not been completely clarified. Presently, an innovative network-pharmacology platform was introduced to systematically elucidate the pharmacological mechanisms of SBH against HCC, adopting active ingredients prescreening, target fishing, and network analysis. The results revealed that SBH appeared to work on HCC probably through regulating 4 molecular functions, 20 biological processes, and hitting on 21 candidate targets involved in 40 pathways. By in-depth analysis of the first-ranked signaling pathway and hit genes, only TTR was highly and specially expressed in the liver tissue. TTR might play a crucial role in neutrophil degranulation pathway during SBH against HCC. Hence, TTR might become a therapeutic target of HCC. The study investigated the anti-hepatoma mechanisms of SBH from a holistic perspective, which provided a theoretical foundation for further experimental research and rational clinical application of SBH.

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“…ESR1 is essential for sexual development and reproductive function and also responsible for bone growth and maintaining normal functions of the cardiovascular and nervous systems. ESR1 mutations or abnormal expression are associated with tumor onset and excessive in ammatory response, and study suggests that luteolin might regulate acute in ammation in renal injury through affecting the expression of ESR1 48 . It is worth noting that the CRP/ALB ratio is a relevant biomarker that re ects microvascular permeability, and CRP/ALB ratio has a strong correlation in assessing cohn's disease activity, analyzing the risk of acute myocardial infarction and predicting mortality in hemodialysis patients [49][50][51][52] .…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of luteolin against inflammation based on integration of network pharmacology, transcriptomics and proteomics

Huang¹,

Zhang²,

Huang³

et al. 2020

Preprint

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Background: Luteolin (3', 4', 5,7-tetrahydroxyflavone), a natural flavonoid exists in various medicinal plants, has strong anti-inflammatory effect. However, anti-inflammatory mechanism of luteolin has not been fully explored. Hence, we aimed to systematically investigate druggability and anti-inflammatory mechanism of luteolin based on network pharmacology. Methods: The absorption, distribution, metabolism, excretion, and toxicity of luteolin were evaluated by TCMSP server. Targets associated with luteolin and inflammation were collected from public databases, and the overlapping targets between luteolin and inflammation were analyzed by Draw Venn diagram. Then the protein-protein interaction network of luteolin against inflammation was constructed to get core genes. Further, gene function and pathway enrichment analysis were performed. Finally, in vitro experiment was carried out to estimate the accuracy of predicted target genes. Results: ADME results indicated that luteolin has great potential to be developed into a drug. 226 overlapping targets (targets of luteolin against inflammation) were screened by matching 280 targets of luteolin with 9015 targets of inflammation. 9 core targets of luteolin against inflammation were identified, including MMP9, MAPK1, HSP90AA1, CASP3, ALB, EGFR, SRC, HRAS and ESR1. Gene function were mainly involved in metabolism, energy pathways and signal transduction. Pathway enrichment results suggested that metabolic pathways, pathways in cancer, PI3K-AKT signaling pathway, Ras signaling pathway and so on might be the critical pathways of luteolin against inflammation. RT-qPCR and ELISA results indicated that luteolin decreased the expression of most of core genes at protein and mRNA levels (MMP9, MAPK1, HSP90AA1, EGFR, SRC and HRAS). Conclusions: The anti-inflammatory mechanism of luteolin were systematically investigated based on network pharmacology, RT-qPCR and ELISA. Luteolin is expounded to have great potential to be developed into a drug and target various genes and pathways to perform systematic anti-inflammatory effect.

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Gene Expression Profiling of Human Kidneys Undergoing Laparoscopic Donor Nephrectomy

Abstract: Background and Objectives: The objective was to compare gene expression profiles of 6 kidneys from open donor nephrectomy with 6 kidneys removed after laparoscopic donor nephrectomy and several hours of carbon dioxide pneumoperitoneum with DNA microarrays and identify small-molecule drugs.

Cited by 4 publications

References 36 publications

Uninephrectomy in rats on a fixed food intake results in adipose tissue lipolysis implicating spleen cytokines

Uninephrectomy in rats on a fixed food intake results in adipose tissue lipolysis implicating spleen cytokines

Systematic Investigation of Scutellariae Barbatae Herba for Treating Hepatocellular Carcinoma Based on Network Pharmacology

Molecular mechanism of luteolin against inflammation based on integration of network pharmacology, transcriptomics and proteomics

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