Gene expression profiling of drug metabolism and toxicology markers using a low-density DNA microarray

Longueville, Françoise de; Surry, Dominic; Meneses-Lorente, Georgina; Bertholet, Vincent; Talbot, Valérie; Evrard, Stéphanie; Chandelier, Nathalie; Pike, Andrew; Worboys, Phil; Rasson, Jean‐Paul; Bourdellès, B. Le; Remacle, José

doi:10.1016/s0006-2952(02)01055-9

Cited by 66 publications

(76 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The value of the technology of low density DNA arrays used in this study has been extensively demonstrated previously in a variety of models, as diverse as hepato-toxicology (de Longueville et al 2002), breast cancer (Gillet et al 2006), skin irritation (Borlon et al 2007b), response to UVB stress (Borlon et al 2007c;Debacq-Chainiaux et al 2005) and cell senescence (de Magalhaes et al 2004;Pascal et al 2005;Zdanov et al 2006). Numerous verifications with real time RT-PCR have been done on all these models.…”

Section: Introductionmentioning

confidence: 80%

“…The variance of the normalized GAPDH was used to generate an estimate of expected variance, leading to a predicted confidence interval for testing the significance of the ratios obtained. Ratios outside the 95% confidence interval were determined to be significantly different using a specifically designed statistical test (de Longueville et al 2002;de Magalhaes et al 2004). …”

Section: Imaging Data Normalization and Analysismentioning

confidence: 99%

See 1 more Smart Citation

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

et al. 2008

View full text Add to dashboard Cite

It is often described that it is difficult to really discriminate the cause of intrinsic skin aging. The aim of this study was to compare the profiles of expression of senescence-associated genes in biopsies of dermis from young and old human donors. TGF-β1 was up-regulated in the dermis of old donors as well as the TGF-β1 -regulated genes. The anti-oxidant enzymes Selenium-dependent Glutathione peroxidase and Glutatione STransferase Theta 1 were also up-regulated in old dermis as well as Tumor Necrosis Factor Receptor Superfamily 1A. None of these genes had altered expression level in skin fibroblasts embedded in a collagen matrix and exposed to sublethal doses of UVB, suggesting their involvement in intrinsic aging. This study represents a proof-of-concept of larger whole transcriptome studies where all avenues should be used to subtract changes in gene expression due to extrinsic aging from changes potentially due to intrinsic aging.

show abstract

Section: Introductionmentioning

confidence: 80%

Section: Imaging Data Normalization and Analysismentioning

confidence: 99%

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The variance of the normalized set of HKGs (except those affected by the tested condition) was used to generate a confidence interval to test the significance of the gene expression ratios (de Longgueville et al 2002). The statistical algorithms of the software are based on a test that was originally developed by Chen et al 1997.…”

Section: Identification Of Leptin-regulated Genesmentioning

confidence: 99%

Leptin-regulated gene expression in MCF-7 breast cancer cells: mechanistic insights into leptin-regulated mammary tumor growth and progression

Perera¹,

Chin²,

Duru³

et al. 2008

Journal of Endocrinology

View full text Add to dashboard Cite

Obesity is a recently established risk factor for breast cancer incidence and mortality. A characteristic of obesity is elevated circulating levels of adipocyte-derived hormone leptin. Evidence indicates that leptin plays an important role in mammary tumor formation; however, the mechanisms involved are poorly understood. Toward better defining the role of leptin in breast cancer, we describe the identification of leptin-regulated genes in hormone-responsive Michigan Cancer Foundation-7 (MCF-7) human breast cancer cells using a microarray system. More than 64 leptin-regulated genes were identified including those for growth factors, cell cycle regulators, extracellular matrix (ECM) proteins, and genes associated with metastasis. Cell cycle genes up-regulated by leptin include cyclins D and G, cyclin-dependent kinase 2, p21, p27, and p16. Leptin suppressed the expression of transforming growth factor-b , a cell cycle suppressor. Determining the significance of this effect, treatment of MCF-7 cells with TGFB1 abrogated leptin-stimulated proliferation. Leptin up-regulated the expression of connective tissue growth factor, villin 2, and basigin, factors that are associated with ECM and are known to impact tumor growth. Finally, leptin induced the expression of anti-apoptotic genes BCL2 and survivin, and reduced the expression of apoptotic genes. The effect of leptin on MCF-7 survival was evaluated via TUNEL assay and demonstrated a sixfold reduction in apoptosis in leptin-treated cells, compared with controls. These data suggest leptin promotes mammary tumor growth through multiple mechanisms, including regulating the cell cycle, apoptosis, and by modulating the extracellular environment. The identification of leptinregulated genes begins to provide mechanistic links into the relationship between obesity and breast cancer incidence and morbidity.

show abstract

“…The use of different intensities allows the quantitation of both the high and low copy expressed genes as described in ref. (17).…”

Section: Expression Profiling Of Abc Transporters With the Dualchip Hmentioning

confidence: 99%

Expression profiling of ATP-binding cassette transporters in childhood T-cell acute lymphoblastic leukemia

Efferth

Gillet

Sauerbrey³

et al. 2006

Molecular Cancer Therapeutics

Self Cite

View full text Add to dashboard Cite

A major issue in the treatment of T-cell acute lymphoblastic leukemia (T-ALL) is resistance to chemotherapeutic drugs. Multidrug resistance can be caused by ATP-binding cassette (ABC) transporters. The majority of these proteins have not yet been examined in T-ALL. Using a newly developed microarray for the simultaneous quantification of 38 ABC transporter genes, we observed a consistent overexpression of ABCA2/ABCA3 in clinical samples of ALL. Therefore, we analyzed the association of these two genes with drug resistance. Treatment of CCRF-CEM and Jurkat cells with methotrexate, vinblastine, or doxorubicin led to an induction of ABCA3 expression, whereas a significant increase of ABCA2 expression was only observed in Jurkat cells. To study the causal relationship of ABCA2/A3 overexpression with drug resistance, we applied RNA interference (RNAi) technology. RNAi specific for ABCA2 or ABCA3 led to a partial decrease of expression in these two ABC transporters. Upon cotreatment of RNAi for ABCA2 with methotrexate and vinblastine, a partial decrease of ABCA2 expression as well as a simultaneous increase of ABCA3 expression was observed. Vice versa, ABCA3 RNAi plus drugs decreased ABCA3 and increased ABCA2 expression. This indicates that down-regulation of one ABC transporter was compensated by the up-regulation of the other. Application of RNAi for both ABCA2 and ABCA3 resulted in a more efficient reduction of the expression of both transporters. As a consequence, a significant sensitization of cells to cytostatic drugs was achieved. In conclusion, ABCA2 and ABCA3 are expressed in many T-ALL and contribute to drug resistance. [Mol Cancer Ther 2006;5(8):1986 -94]

show abstract

Gene expression profiling of drug metabolism and toxicology markers using a low-density DNA microarray

Cited by 66 publications

References 39 publications

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study

Leptin-regulated gene expression in MCF-7 breast cancer cells: mechanistic insights into leptin-regulated mammary tumor growth and progression

Expression profiling of ATP-binding cassette transporters in childhood T-cell acute lymphoblastic leukemia

Contact Info

Product

Resources

About