Gene expression profiling of common signal transduction pathways affected by rBMSCs/F92A-Cav1 in the lungs of rat with pulmonary arterial hypertension

Chen, Haiying; Yang, Hongli; Xu, Chong; Yue, Hongmei; Xia, Peng; Strappe, Pádraig; Wang, Lei; Pan, Li; Tang, Wenqiang; Chen, Shuangfeng; Wang, Lexin

doi:10.1016/j.biopha.2016.06.028

Cited by 5 publications

(3 citation statements)

References 26 publications

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“…Lentiviral vector packaging and transduction was performed as described previously with minor modifications (12). For the generation of LV-PGK and LV-ZNF750 lentivirus, 7 µg PGK or ZNF750 lentiviral vector plasmid together with packaging plasmids (7 µg psPax2, 3 µg pRSV-Rev and 3 µg VSV-G) were co-transfected into 70-80% confluent 293T cells, using Lipofectamine ® 2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA).…”

Section: Lentiviral Packaging and Cal-27 Cell Transductionmentioning

confidence: 99%

Overexpression of tumor suppressor gene ZNF750 inhibits oral squamous cell carcinoma metastasis

Yang

Pan

et al. 2017

Oncol Lett

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Abstract. Zinc-finger protein 750 (ZNF750) encodes a putative C2H2 zinc finger protein and is typically mutated or deleted in squamous cell carcinoma. The role of ZNF750 in oral squamous cell carcinoma (OSCC) remains unknown. The aim of the present study was to investigate the effects of ZNF750 overexpression in CAL-27 cells. Cell viability, and the expression of genes associated with proliferation, differentiation and the epithelial-mesenchymal transition were investigated in CAL-27 cells following ZNF750 overexpression, using Cell Counting kit-8, reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. In addition, scratch wound, invasion and migration assays were performed. Cell viability, matrix metalloproteinase 28 expression, cyclin B1 expression and mesenchymal marker neural cadherin expression were decreased following ZNF750 overexpression compared with the control groups. ZNF750 overexpression induced the differentiation-associated genes late cornified envelope 3A and small proline-rich protein 1A and upregulated the expression of late epidermal differentiation factor Kruppel-like factor 4. Overexpression of ZNF750 in CAL-27 cells resulted in inhibition of cell invasion and migration. Taken together, these data suggest that ZNF750 may inhibit the metastasis of OSCC.

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Section: Lentiviral Packaging and Cal-27 Cell Transductionmentioning

confidence: 99%

Overexpression of tumor suppressor gene ZNF750 inhibits oral squamous cell carcinoma metastasis

Yang

Pan

et al. 2017

Oncol Lett

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“…Also, elevated expression of hsa‐miR‐599 triggers restricted proliferation, migration, and invasion of HCC cells in vitro (J. Tian et al, ). Hey2 along with Bcl‐2 is found to be a pro‐proliferation gene in pulmonary arterial hypertension (H. Chen et al, ). Overexpressed Hey‐2 has been detected to enhance cell proliferation and attenuate apoptosis in cerebral ischemic stroke in vitro (Liu et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Tian et al, 2016). Hey2 along with Bcl-2 is found to be a pro-proliferation gene in pulmonary arterial hypertension (H. Chen et al, 2016). Overexpressed Hey-2 has been detected to enhance cell proliferation and attenuate apoptosis in cerebral ischemic stroke in vitro (Liu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

microRNA‐599 promotes apoptosis and represses proliferation and epithelial‐mesenchymal transition of papillary thyroid carcinoma cells via downregulation of Hey2‐depentent Notch signaling pathway

Wang

Tang

Liang

et al. 2019

Journal Cellular Physiology

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Although papillary thyroid carcinoma (PTC) has a favorable prognosis after surgical or medical treatment, its survival rate is still very low. Therefore, finding more reliable therapy methods to limit PTC is a necessity. Compelling evidence has implicated the role of microRNAs (miRNAs or miRs) in PTC. This study aims at investigating the possible effect of microRNA-599 (miR-599) on proliferation, apoptosis, and epithelialmesenchymal transition (EMT) of PTC cells by targeting Hey2 gene. Differentially expressed genes/miRNAs associated with PTC were screened based on microarray analysis. Then, the expression of the candidate gene, as well as, the regulatory miRNA were detected in PTC cells, the related signaling pathway was verified. Afterward, the relationship between the miR and the candidate gene was verified by dual-luciferase reporter gene assay. Subsequently, the effects of overexpressed miR and silenced candidate gene on cell proliferation, cell apoptosis, EMT, migration, and invasion were detected. In PTC tissues and cells, miR-599 was downregulated while Hey2 expressed highly. Hey2 is a target gene of miR-559. In addition, the expression of Bax and E-cadherin was elevated while that of Hey2, Notch1, Delta-like1, Hes1, N1ICD, Jagged1, Snail, Slug, N-cadherin and Vimentin, and Bcl-2 was reduced in cells treated with upregulated miR-599 or downregulated Hey2. Moreover, miR-599 overexpression or Hey2 silencing inhibited cell proliferation, migration, invasion, along with EMT but promoted apoptosis. This study verified that miR-599 promotes apoptosis and represses proliferation, EMT of PTC cells through inactivating the Notch signaling pathway by downregulating Hey2, which has great clinical significance for PTC treatment. K E Y W O R D S epithelial-mesenchymal transition, Hey2, microRNA-599, Notch signaling pathway, papillary thyroid carcinoma, proliferation

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Integrin alpha5beta1 suppresses rBMSCs anoikis and promotes nitric oxide production

Chen

Pan

Yang

et al. 2018

Biomedicine & Pharmacotherapy

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Gene expression profiling of common signal transduction pathways affected by rBMSCs/F92A-Cav1 in the lungs of rat with pulmonary arterial hypertension

Cited by 5 publications

References 26 publications

Overexpression of tumor suppressor gene ZNF750 inhibits oral squamous cell carcinoma metastasis

Overexpression of tumor suppressor gene ZNF750 inhibits oral squamous cell carcinoma metastasis

microRNA‐599 promotes apoptosis and represses proliferation and epithelial‐mesenchymal transition of papillary thyroid carcinoma cells via downregulation of Hey2‐depentent Notch signaling pathway

Integrin alpha5beta1 suppresses rBMSCs anoikis and promotes nitric oxide production

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