Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells

Seo, Dong‐Cheol; Sung, Jong‐Hyuk; Cho, Hee-Jung; Yi, Hee; Seo, Kun‐Ho; Choi, In‐Soo; Kim, Dong-Ku; Kim, Jin-Suk; El‐Aty, A. M. Abd; Shin, Ho‐Chul

doi:10.1186/1476-4598-6-75

Cited by 82 publications

(65 citation statements)

References 39 publications

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“…Furthermore, CD338 can promote resistance to multiple drugs and has been observed in many types of TICs (Bertolini et al, 2009). Lung cancer stem cells, isolated with Hoechst 33342, were found to express significantly higher levels of CD338 (Seo et al, 2007). In our study, we found that the TICs we isolated strongly had highly expressed CD133 + /CD338 + , possessed proliferation and enhanced tumorigenesis capacities, and more highly expressed of stem cell-associated genes.…”

Section: Discussionsupporting

confidence: 52%

Monitoring microRNAs Using a Molecular Beacon in CD133⁺/CD338⁺Human Lung Adenocarcinoma-initiating A549 Cells

Yao

Sun²,

Ma³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Discussionsupporting

confidence: 52%

Monitoring microRNAs Using a Molecular Beacon in CD133⁺/CD338⁺Human Lung Adenocarcinoma-initiating A549 Cells

Yao

Sun²,

Ma³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Recently, Wang et al showed that knockdown of IL-6R diminished stemness and survival of glioma stem cells (26). In our previous studies, we have isolated lung CSCs using the Hoechst 33342 exclusion assay and demonstrated that IL-6R expression is upregulated in CSCs (27,28).…”

Section: Introductionmentioning

confidence: 99%

Blockade of interleukin-6 receptor suppresses the proliferation of H460 lung cancer stem cells

Cho

et al. 2012

Int J Oncol

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Abstract. IL-6/6R signaling is closely associated with tumor growth and poor prognosis. Although there is evidence that interleukin-6 receptor (IL-6R)-mediated signaling promotes the growth and malignancy of cancer, the role of IL-6R in cancer stem cells (CSCs) is poorly defined. This study investigated the role of IL-6R in the proliferation of CSCs. Sphere-forming cells were isolated from the H460 non-small cell lung cancer (NSCLC) cell line and identified as CSCs using confocal microscopy, RT-PCR and WST-1 assay. The H460 spheres demonstrated the typical characteristics of CSCs, including CD133 expression, upregulation of Nanog, self-renewal, and drug resistance to methotrexate (MTX) and fluorouracil (5-FU). The release of IL-6R and its ligand, IL-6, were quantitatively determined and compared between CSCs and non-CSCs. The concentration of soluble IL-6R (sIL-6R) was remarkably high in CSCs compared to that in non-CSCs. Furthermore, significant upregulation of the IL-6R gene was also observed in the CSCs. The growth of CSCs was significantly inhibited by transfection with IL-6R small-interfering RNA (siRNA), as well as with the IL-6R monoclonal antibody (mAb). In addition, blocking both IL-6R and IL-6 using siRNA or mAbs intensified the inhibition of CSC proliferation. These findings indicate that IL-6R is present in CSCs and has an important role in the proliferation of CSCs in the H460 lung cancer cell line. Therefore, we suggest that IL-6R is both a viable target for the development of CSC-directed lung cancer therapeutics and a potential CSC marker in NSCLC.

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“…To date, however, any markers useful for detection of CSCs in lung cancer have not been known. Recently, Seo et al 22 reported that several genes were highly expressed in SP of lung adenocarcinoma cell lines: one of them was the gene for NR0B1, a recently characterized member of the orphan nuclear receptor family. 23 NR0B1 (also called dosage-sensitive sex reversal/adrenal hypoplasia congenital critical region on the X chromosome 1; DAX1) acts as a negative regulator of steroid production, and is expressed in the reproductive and endocrine systems.…”

mentioning

confidence: 99%

Tumorigenic Role of Orphan Nuclear Receptor NR0B1 in Lung Adenocarcinoma

Oda

Tian

Inoue

et al. 2009

The American Journal of Pathology

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Cancer stem cells are a limited population of tumor cells that are thought to reconstitute whole tumors. The Hoechst dye exclusion assay revealed that tumors are composed of both a main population and a side population of cells, which are rich in cancer stem cells. NR0B1 is an orphan nuclear receptor that is expressed to a greater extent in the side population, as compared with the main population, of a lung adenocarcinoma cell line. In this study, we investigated the role of NR0B1 in lung adenocarcinoma cells. Reduction of NR0B1expression levels in lung adenocarcinoma cell lines resulted in vulnerability to anticancer drugs and decreased abilities for invasion, in vitro colony formation, and tumorigenicity in nonobese diabetic/severe compromised immunodeficient mice. When 193 cases of lung adenocarcinoma were immunohistochemically examined, higher levels of NR0B1 expression correlated with higher rates of lymph node metastasis and recurrence. Multivariate analysis revealed high NR0B1 expression levels, stage of the disease, and size of tumor to be independent unfavorable prognostic factors for overall and disease-free survival rates. In clinical samples, NR0B1 expression levels inversely correlated to the proportion of methylated CpG sequences around the transcription initiation site of the NR0B1 gene, suggesting the epigenetic control of NR0B1 transcription in lung adenocarcinoma. In conclusion, NR0B1 might play a role in the malignant potential of lung adenocarcinoma. (Am J

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Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells

Abstract: Background: The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells.

Cited by 82 publications

References 39 publications

Monitoring microRNAs Using a Molecular Beacon in CD133⁺/CD338⁺Human Lung Adenocarcinoma-initiating A549 Cells

Monitoring microRNAs Using a Molecular Beacon in CD133⁺/CD338⁺Human Lung Adenocarcinoma-initiating A549 Cells

Blockade of interleukin-6 receptor suppresses the proliferation of H460 lung cancer stem cells

Tumorigenic Role of Orphan Nuclear Receptor NR0B1 in Lung Adenocarcinoma

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Gene expression profiling of cancer stem cell in human lung adenocarcinoma A549 cells

Abstract: Background: The studies on cancer-stem-cells (CSCs) have attracted so much attention in recent years as possible therapeutic implications. This study was carried out to investigate the gene expression profile of CSCs in human lung adenocarcinoma A549 cells.

Cited by 82 publications

References 39 publications

Monitoring microRNAs Using a Molecular Beacon in CD133+/CD338+Human Lung Adenocarcinoma-initiating A549 Cells

Monitoring microRNAs Using a Molecular Beacon in CD133+/CD338+Human Lung Adenocarcinoma-initiating A549 Cells

Blockade of interleukin-6 receptor suppresses the proliferation of H460 lung cancer stem cells

Tumorigenic Role of Orphan Nuclear Receptor NR0B1 in Lung Adenocarcinoma

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Monitoring microRNAs Using a Molecular Beacon in CD133⁺/CD338⁺Human Lung Adenocarcinoma-initiating A549 Cells

Monitoring microRNAs Using a Molecular Beacon in CD133⁺/CD338⁺Human Lung Adenocarcinoma-initiating A549 Cells