Gene Expression Profiling of Broiler Liver under Cold Stress by High-Throughput Sequencing Technology

Abstract

Cold stress is a major environmental factor restricting the sustainable development of animal husbandry. To gain insight into the gene-regulation processes in broilers under cold stress, gene expression profiling was conducted using high-throughput Solexa sequencing of broiler liver tissue under cold stress conditions and control conditions. According to Solexa sequencing, we identified 255 genes whose expression levels differed between the treatment and control group. Under cold stress, 135 genes were up-regulated and 120 genes were down-regulated genes compared with levels in the control group. Moreover, 469 genes were expressed only in the control group, and 172 genes were expressed only in the treatment group. These data were confirmed by real-time quantitative PCR. Gene Ontology enrichment analysis showed that the differentially expressed genes (DEGs) were mainly enriched in material metabolism and immune functions. KEGG enrichment analysis showed that DEGs were enriched in pyruvate metabolism, glycolysis/gluconeogenesis, fatty acid metabolism，insulin signaling pathway and others. In conclusion, these results may serve as an important reference for broiler breeding and provide new clues for the elucidation of molecular mechanisms of cold stress.

Transcriptomic analysis of the liver in aged laying hens with different intensity of brown eggshell color

“…For poultry, the RNA-seq techniques have been adopted to compare the overall transcriptomic alteration in the liver of broiler chickens exposed to different temperature stress (e.g. heat stress or cold stress) [6,7] and laying hens at different…”

Transcriptomic analysis of the liver in aged laying hens with different eggshell strength

“…The application of transcriptomic analysis in poultry science is recently increasing because this analysis can provide a useful information for the molecular changes based on the identification of vital genes and their expression levels, which are related metabolic pathways for poultry raised under different environment and managements ( Coble et al, 2014 ; Li et al., 2015a ; Li et al, 2017 ; Wan et al, 2017 ). Several previous studies have performed transcriptomic analysis to reveal the molecular mechanisms related to eggshell strength of laying hens with an emphasis of uterine tissues where eggs are actually formed ( Sah et al, 2018 ; Feng et al, 2020 ).…”

The Magnetic Leakage Inversion Method Based on Singular Value Decomposition of Magnetic Dipole Forward Model

“…However, the economic losses caused by the defects of ferromagnetic materials are numerous every year. Among them, magnetic flux leakage detection [1][2] has been widely used in defect detection of ferromagnetic materials due to its advantages such as high sensitivity, low cost, fast speed, low requirements on workpiece surface cleanliness. Leakage magnetic inversion [3][4] , that is, the location and size of the defect are calculated according to the known leakage magnetic field signals, is the solution process of electromagnetic field inverse problem.…”