Gene expression profiling of bovine preovulatory follicles: gonadotropin surge and prostanoid-dependent up-regulation of genes potentially linked to the ovulatory process

Li, Qinglei; Jimenez-Krassel, Fermin; Ireland, James J.; Smith, George W.

doi:10.1530/rep-08-0308

Cited by 37 publications

(35 citation statements)

References 82 publications

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“…Factors from the EGF family, known as EGF-like growth factors, are immediately upregulated by LH surge in vivo and also after LH treatment in follicular cell cultures (Park et al 2004, Li et al 2009). Amphiregulin (AREG) and epiregulin (EREG) are two of the LH-induced genes mediating the gonadotropin-induced PTGS2 expression (Park et al 2004, Andric & Ascoli 2008, Li et al 2009).…”

Section: Ang II Signaling and Receptors Near Ovulationmentioning

confidence: 99%

“…Amphiregulin (AREG) and epiregulin (EREG) are two of the LH-induced genes mediating the gonadotropin-induced PTGS2 expression (Park et al 2004, Andric & Ascoli 2008, Li et al 2009). The hypothesis that Ang II is a mediator of LH action in the cascade of events leading to ovulation was confirmed by our group using bovine granulosa cell cultures derived from O10 mm follicles.…”

Section: Ang II Signaling and Receptors Near Ovulationmentioning

confidence: 99%

See 1 more Smart Citation

Role of angiotensin in ovarian follicular development and ovulation in mammals: a review of recent advances

Gonçalves¹,

Ferreira²,

Gasperin³

et al. 2012

Reproduction

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Angiotensin (Ang) II is widely known for its role in the control of systemic blood vessels. Moreover, Ang II acts on the vascular control of ovarian function, corpus luteum formation, and luteolysis. Over the past 10 years, our research group has been studying the new concept of the renin-angiotensin system (RAS) as an autocrine/paracrine factor regulating steroidogenesis and promoting different cellular responses in the ovary, beyond vascular function. We have developed and used different in vivo and in vitro experimental models to study the role of RAS in the ovary and a brief overview of our findings is presented here. It is widely accepted that there are marked species differences in RAS function in follicle development. Examples of species-specific functions of the RAS in the ovary include the involvement of Ang II in the regulation of follicle atresia in rats vs the requirement of this peptide for the dominant follicle development and ovulation in rabbits and cattle. More recently, Ang-(1-7), its receptor, and enzymes for its synthesis (ACE2, NEP, and PEP) were identified in bovine follicles, implying that Ang-(1-7) has an ovarian function. Other novel RAS components (e.g. (pro)renin receptor and renin-binding protein) recently identified in the bovine ovary show that ovarian RAS is poorly understood and more complex than previously thought. In the present review, we have highlighted the progress toward understanding the paracrine and autocrine control of ovarian antral follicle development and ovulation by ovarian tissue RAS, focusing on in vivo studies using cattle as a model.

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Section: Ang II Signaling and Receptors Near Ovulationmentioning

confidence: 99%

Section: Ang II Signaling and Receptors Near Ovulationmentioning

confidence: 99%

Role of angiotensin in ovarian follicular development and ovulation in mammals: a review of recent advances

Gonçalves¹,

Ferreira²,

Gasperin³

et al. 2012

Reproduction

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“…In agreement, PGE2 induces AREG and EREG in a dose-dependent fashion in human GCs (Ben-Ami et al 2006). Moreover, injecting intrafollicular indomethacin (INDO) in cows showed reduced AREG expression in GCs (Li et al 2009). These reports tend to suggest that PTGS2 induces EGF-like factors.…”

Section: Discussionmentioning

confidence: 53%

Regulation of ovulatory genes in bovine granulosa cells: lessons from siRNA silencing of PTGS2

Shrestha¹,

Łukasik²,

Baufeld³

et al. 2015

Reproduction

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Prostaglandin endoperoxide synthase-2 (PTGS2), tumour necrosis factor-alpha-induced protein-6 (TNFAIP6), pentraxin-3 (PTX3), epidermal growth factor-like factors: amphiregulin (AREG) and epiregulin (EREG) are essential for successful ovulation. In this study, we compared the induction of these ovulatory genes in bovine granulosa cells (GCs) in vivo (after LH surge) and in vitro (forskolin (FRS) treatment). These genes were markedly stimulated in GCs isolated from cows 21 h after LH-surge. In isolated GCs, FRS induced a distinct temporal profile for each gene. Generally, there was a good agreement between the in vivo and in vitro inductions of these genes except for PTX3. Lack of PTX3 induction in isolated GCs culture suggests that other follicular compartments may mediate its induction by LH. Next, to study the role of PTGS2 and prostaglandins (PGs) in the cascade of ovulatory genes, PTGS2 was silenced with siRNA. PTGS2 siRNA caused a marked and specific knockdown of PTGS2 mRNA and PGE2 production (70% compared with scrambled siRNA) in bovine GCs. Importantly, PTGS2 silencing also reduced AREG, EREG and TNFAIP6 mRNA levels but not PTX3. Exogenous PGE2 increased AREG, EREG and TNFAIP6 mRNA levels, further confirming that these genes are prostanoid dependent. A successful and specific knockdown of PTGS2 was also achieved in endometrial cells (EndoCs) expressing PTGS2. Then, cholesterol-conjugated PTGS2 (chol-PTGS2) siRNA that facilitates cells' entry was investigated. In EndoCs, but not in GCs, chol-PTGS2 siRNA succeeded to reduce PTGS2 and PGE2 levels even without transfection reagent. PTGS2 knockdown is a promising tool to critically examine the functions of PTGS2 in the reproductive tract.

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“…Among them are the 2 members of ADAM metallopeptidases. The ADAM play a role in bovine preovulatory follicles during the LH surge (LI, et al, 2009;BLISS, et al 2010). The ADAMs 10 and 17 were described as regulators of LH function control on follicular cells (LI, et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The ADAM play a role in bovine preovulatory follicles during the LH surge (LI, et al, 2009;BLISS, et al 2010). The ADAMs 10 and 17 were described as regulators of LH function control on follicular cells (LI, et al, 2009). Other members of ADAM family (ADAM 11 and 33) were more expressed in the estradiol presence on the preoptic area, suggesting that ADAMs 11 and 33 can regulate the GnRH release and control LH surge.…”

Section: Discussionmentioning

confidence: 99%

Estudo do mecanismo molecular da progesterona e do estradiol sobre o início da puberdade em novilhas Nelore

Magalhães¹

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AGRADECIMENTOSAgradeço a Deus e a Nossa Senhora por me fazerem forte, me manterem firme, segura dos meus objetivos, e persistente. Agradeço toda a proteção e às bênçãos recebidas.À minha família, meu maior tesouro, meu chão, meu mundo. Em especial à minha mãe, Leila, meu porto seguro e meu maior apoio, a quem tanto amo. Ao meu pai que me apresentou outro mundo e a quem tenho aprendido a compreender. Aos meus avós, Maria e Afonso, fontes de inspiração, alicerces da nossa família, exemplos de união e amores da minha vida. À minha avó Estelita, muitas saudades e ao avô Ernesto que não tive oportunidade de conhecer. Aos meus tios e padrinhos, Keila (amiga inseparável e verdadeira), Marciano (incentivador das minhas idéias mais malucas), tio Júnior (pai dos meus amores), Marlon, Sônia, Netinho, Smênia, Valmira, Xandy, Néia, Vanusa, Alípio, Toninho, Mara, eternos cúmplices, amigos, apoios, pessoas que ajudaram a formar minha personalidade e me ajudaram a ser uma pessoa melhor, obrigada por confiarem em mim. Aos meus irmãos Newton, Daniel, Grégory, parceiros eternos de vida, farras, bons momentos e dificuldades e às minhas irmãs Isabela e Lívia, minhas lindas! Amo a todos vocês! Aos meus primos lindos, Neto, Gu, Bia, Arthur, Lorrane, Igor, Fred, Breno, Grazian, Dani (e família), amo vocês demais apesar da distância. Aos meus dois afilhados Arthurzinho e Gu, que me desmontam a cada passagem por Brasília. E o agradecimento mais especial aos novos presentes de Deus que trouxeram vida nova e alegria à família meus sobrinhos Esther, Enzo e Anna.A todos os meus amigos de Brasília, que entendem o motivo da minha distância, mas não menor consideração.À FAPESP pela concessão da bolsa de doutorado e pelo auxílio financeiro à pesquisa, que me possibilitaram ter tranquilidade no desenvolvimento de minhas atividades. À Prefeitura do Campus da USP de Pirassununga por disponibilizar as instalações do abatedouro escola e parte dos animais.Ao professor Luis Felipe Prada e Silva, por todo o apoio, confiança, amizade e estrutura, sem os quais não seria possível o desenvolvimento do trabalho. E por todo o aprendizado e vivência fora do projeto de pesquisa, mas dentro do contexto do LPGC, que só me auxiliam a crescer e me fazem uma melhor profissional a cada dia. The understanding of the molecular mechanisms by which nutrition, genetics and hormonal treatments affect the beginning of puberty is of great importance for developing strategies aiming to reduce the age at first calving, and therefore increase the slaughter rate in Nellore cattle. The effects of progesterone device and of endogenous estradiol on the molecular mechanisms controlling the attainment of puberty in Nellore heifers were investigated.Specifically, the molecular pathways of progesterone and estradiol were studied in the hypothalamus. Thirty five non-pubertal heifers, between 13 and 14 months of age, were divided into four treatment (nine or eight per treatment): P4 device without estradiol (SP), P4 device with estradiol (PE), without P4 device and without estradiol (SS...

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Gene expression profiling of bovine preovulatory follicles: gonadotropin surge and prostanoid-dependent up-regulation of genes potentially linked to the ovulatory process

Cited by 37 publications

References 82 publications

Role of angiotensin in ovarian follicular development and ovulation in mammals: a review of recent advances

Role of angiotensin in ovarian follicular development and ovulation in mammals: a review of recent advances

Regulation of ovulatory genes in bovine granulosa cells: lessons from siRNA silencing of PTGS2

Estudo do mecanismo molecular da progesterona e do estradiol sobre o início da puberdade em novilhas Nelore

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