Gene expression profiling of avian macrophage activation

Bliss, Travis W.; Dohms, John E.; Emara, Marlene G.; Keeler, Calvin L.

doi:10.1016/j.vetimm.2005.02.013

Cited by 64 publications

(46 citation statements)

References 28 publications

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“…One of the downregulated genes is carboxypeptidase M, a macrophage differentiation marker [17]. Also similar to ORF2 (LOC428036) was downregulated, whereas this gene was shown before to be upregulated in avian macrophages after phagocytosis of Escherichia coli [7], so lower expression levels of this genes might indicate a decrease in the amount of macrophages. This is the first report about changes in T-cell subpopulations and macrophages in the chicken jejunum in response to a Salmonella infection in one day old broilers and these changes were different between the two lines used.…”

Section: Discussionmentioning

confidence: 98%

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

et al. 2007

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-Besides infection in humans, Salmonella enteritidis can also cause serious illness in young chickens. However, the genetic and immunological parameters important for the disease in chickens are not well characterized. In this study, processes in the chicken intestine in response to a Salmonella infection were investigated in two different chicken lines. One-day-old chickens were orally infected with Salmonella. T-cell subpopulations, phagocytic properties of intestinal mononuclear cells and RNA expression levels of the jejunum were investigated. The two chicken lines differed in the amount of cfu in the liver and growth retardation after the infection. Differences in phagocytic activity of intestinal mononuclear cells were found between control and Salmonella infected chickens. The number of CD4 + T-cells of the intestine decreased after the Salmonella infection in one chicken line, while the number of CD8 + T-cells increased in both chicken lines, but the time post infection of this increase differed between the lines. In one chicken line the expression levels of the genes carboxypeptidase M and similar to ORF2 decreased after the Salmonella infection, which might be related to a decrease in the amount of macrophages. With the microarray, ten genes were found that were regulated in only one of the chicken lines, while we found six genes regulated in response to the infection in both chicken lines. So differences in genetic background of the chickens influence the intestinal host response of the Salmonella infection as observed by phagocytic activity, gene expression and changes in the number of T-cell subpopulations and macrophages.immunology / microarray / Salmonella / T-cell

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Section: Discussionmentioning

confidence: 98%

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

et al. 2007

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“…Fundamentally, the transcription factor, NF-κB has played a pivotal role in mediation of genes related to the inflammatory process, such as IL-1β, IL-6 or iNOS (Bliss et al, 2005;Kobayashi et al, 2002). Furthermore, the increased expressions of many genes involved in phagocytosis are observed during the first few hours after induction (Kobayashi et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Srikok

Nambut

Wongsawan

et al. 2017

American Journal of Animal and Veterinary Sciences

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This study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 µM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions of CORO1A, CYBA (gp91 phox ), LAMP1, RAB7A, RAC1 and PAK1 mRNA were analyzed by real-time PCR. In the time-course experiment, treated neutrophils were allowed to co-cultured with live bacteria for 30, 60 and 90 min before measuring gene expressions. The expression levels of IL-1 β and TNF genes in order to demonstrate the antiinflammatory property of quercetin were assessed by conventional RT-PCR. The results of flow cytometry and microscopic examination showed that the percentage of neutrophils performing phagocytosis were significantly higher in QH group in comparison with other groups. We reported here that mRNA expressions of CORO1A, CYBA, LAMP1, RAB7A, RAC1 and PAK1 genes involved in phagocytosis, were significantly up-regulated in QH group. As expected, CytB group had profound down-regulation of genes with one exception in PAK1. From our observations, the expression levels of phagocytic process genes in the QH group were optimum at 60 min and started to decline at 90 min of incubation. The data also indicated that quercetin inhibited inflammation by reducing the expressions of both IL-1β and TNF. In conclusion, the information in our experiments conclude that quercetin has the ability to boost the expression of genes involved in phagocytosis while reduces the expression of proinflammatory genes.

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“…In gene expression studies, using different fluorescent labels for different samples enables the comparison of gene expression between groups and thus differential gene expression. Microarrays have been developed to study the expression of genes involved in a wide range of functions in avian model species, such as bird song and immune reactions (Afrakhte and Schultheiss 2004;Bliss et al 2005;Burnside et al 2005;Li et al 2008;Neiman et al 2001;Smith et al 2006;Van Hemert et al 2003;Wada et al 2006;Wade et al 2004). Microarrays customised for avian model species such as Zebra Finch Taeniopygia guttata (Naurin et al 2008;Wada et al 2006) have further been used for related species (termed 'cross-species microarray analysis') (Cheviron et al 2008;Naurin et al 2012).…”

Section: Microarray Analysismentioning

confidence: 99%

Avian transcriptomics: opportunities and challenges

Jax

Kraus

2018

J Ornithol

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Recent developments in next-generation sequencing technologies have greatly facilitated the study of whole transcriptomes in model and non-model species. Studying the transcriptome and how it changes across a variety of biological conditions has had major implications for our understanding of how the genome is regulated in different contexts, and how to interpret adaptations and the phenotype of an organism. The aim of this review is to highlight the potential of these new technologies for the study of avian transcriptomics, and to summarise how transcriptomics has been applied in ornithology. A total of 81 peer-reviewed scientific articles that used transcriptomics to answer questions within a broad range of study areas in birds are used as examples throughout the review. We further provide a quick guide to highlight the most important points which need to be take into account when planning a transcriptomic study in birds, and discuss how researchers with little background in molecular biology can avoid potential pitfalls. Suggestions for further reading are supplied throughout. We also discuss possible future developments in the technology platforms used for ribonucleic acid sequencing. By summarising how these novel technologies can be used to answer questions that have long been asked by ornithologists, we hope to bridge the gap between traditional ornithology and genomics, and to stimulate more interdisciplinary research.

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Gene expression profiling of avian macrophage activation

Cited by 64 publications

References 28 publications

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

Immunological and gene expression responses to aSalmonellainfection in the chicken intestine

Quercetin Promotes the Expression of Genes Involved in Phagocytosis in Bovine Neutrophils

Avian transcriptomics: opportunities and challenges

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