Gene Expression Profiling in Response to Ultraviolet Radiation in Maize Genotypes with Varying Flavonoid Content

Casati, Paula; Walbot, Virginia

doi:10.1104/pp.103.022871

Cited by 207 publications

(207 citation statements)

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“…In addition, increasing evidence indicates that RPL10 protein from various organisms has multiple extraribosomal functions, besides being a constituent of the ribosome and participating in protein synthesis (Chan et al, 1996;Wool, 1996;Nika et al, 1997;Mills et al, 1999;Chávez-Ríos et al, 2003;Wen et al, 2005). Previously, by transcriptome profiling using different UV-B regimes and maize lines, we found that the functional group with the largest number of genes up-regulated by UV-B light corresponds to proteins involved in protein synthesis, including cytoplasmic ribosomal proteins, initiation and elongation factors, and poly(A)-binding proteins (Casati and Walbot, 2003). Among them, rpl10, a homolog of a human transcript encoding the QM protein, showed increased levels after the UV-B treatments (Casati and Walbot, 2003).…”

Section: Discussionmentioning

confidence: 89%

“…In previous experiments, by microarray analysis, we showed that a maize rpl10 gene is induced by UV-B radiation (Casati and Walbot, 2003). To validate this result, we analyzed the effect of UV-B radiation on the expression of RPL10 genes in maize and Arabidopsis plants by qRT-PCR.…”

Section: Regulation Of Rpl10 Expression By Uv-b Lightmentioning

confidence: 85%

“…Previously, by transcriptome profiling using different UV-B regimes and maize lines, we found that the functional group with the largest number of genes up-regulated by UV-B light corresponds to proteins involved in protein synthesis, including cytoplasmic ribosomal proteins, initiation and elongation factors, and poly(A)-binding proteins (Casati and Walbot, 2003). Among them, rpl10, a homolog of a human transcript encoding the QM protein, showed increased levels after the UV-B treatments (Casati and Walbot, 2003). In this work, we identified two rpl10 genes in maize, while Arabidopsis has a gene family with three members (RPL10A to RPL10C).…”

Section: Discussionmentioning

confidence: 99%

“…Previously, by transcriptome profiling, the expression of a number of ribosomal proteins was found to be up-regulated by UV-B light in maize (Zea mays) plants exposed under different light regimes (Casati and Walbot, 2003). In those experiments, rpl10 was found to be up-regulated by UV-B light.…”

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confidence: 97%

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Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

et al. 2010

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(J.B., A.L.B.) Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.

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Section: Discussionmentioning

confidence: 89%

Section: Regulation Of Rpl10 Expression By Uv-b Lightmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

et al. 2010

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“…Previously, using microarray hybridization to assess maize (Zea mays) acclimation responses to UV-B, we found that genes encoding protein translation components were the largest functional group up-regulated by UV-B (Casati and Walbot, 2003). On a microarray containing approximately 2,500 distinct maize cDNA types, there was significant up-regulation of 44 transcripts associated with translation or ribosome assembly.…”

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Crosslinking of Ribosomal Proteins to RNA in Maize Ribosomes by UV-B and Its Effects on Translation

Casati

Walbot

2004

Plant Physiology

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Ultraviolet-B (UV-B) photons can cause substantial cellular damage in biomolecules, as is well established for DNA. Because RNA has the same absorption spectrum for UV as DNA, we have investigated damage to this cellular constituent. In maize (Zea mays) leaves, UV-B radiation damages ribosomes by crosslinking cytosolic ribosomal proteins S14, L23a, and L32, and chloroplast ribosomal protein L29 to RNA. Ribosomal damage accumulated during a day of UV-B exposure correlated with a progressive decrease in new protein production; however, de novo synthesis of some ribosomal proteins is increased after 6 h of UV-B exposure. After 16 h without UV-B, damaged ribosomes were eliminated and translation was restored to normal levels. Ribosomal protein S6 and an S6 kinase are phosphorylated during UV-B exposure; these modifications are associated with selective translation of some ribosomal proteins after ribosome damage in mammalian fibroblast cells and may be an adaptation in maize. Neither photosynthesis nor pigment levels were affected significantly by UV-B, demonstrating that the treatment applied is not lethal and that maize leaf physiology readily recovers.The evolution of terrestrial life was made possible by formation of a stratosphere that screens solar UV radiation, absorbing UV-C (,280 nm) and the most energetic UV-B (280-315 nm). Recent depletion of stratospheric ozone by chlorofluorocarbons and other pollutants has increased terrestrial UV-B levels with potentially deleterious consequences for all living organisms and particularly for plant development and physiology (Ballaré et al., 2001;Searles et al., 2001;Paul and Gwynn-Jones, 2003). In response to the inevitable exposure to damaging terrestrial UV-B, plants have evolved UV-induced mechanisms of protection and repair, such as accumulation of UVabsorbing pigments and use of UV-A photons to repair UV-B induced DNA damage (Stapleton and Walbot, 1994;Britt, 1996). Because of its absorption spectrum, DNA is a major target of UV-B radiation (Britt, 1996). This radiation can also damage proteins and lipids directly (Gerhardt et al., 1999); RNA molecules strongly absorb UV-B, but less is known about UV-B mediated damage to this cellular constituent.Experimentally, UV has been extensively used to analyze ribosome structure in vitro, because crosslinks can be introduced at points of close contact between proteins, within ribosomal RNA (rRNA), and between proteins and rRNA, tRNA, or mRNA Noah et al., 2000). Furthermore, in vivo crosslinks are generated in rRNA by UV treatment of bacterial cells (Stiege et al., 1986). In mammalian cells, UV-C and UV-B cause specific damage to the 3#-end of the 28S rRNA activating a response called ribotoxic stress (Iordanov et al., 1997(Iordanov et al., , 1998. Protein synthesis in plant cells is also sensitive to UV-C. Murphy et al. (1973) demonstrated that 254 nm radiation disrupted amino acid incorporation into protein in a wheat germ in vitro synthesis reaction and that the most sensitive target was mRNA. Subsequent studies in cult...

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Plant Phenolics: A Biochemical and Physiological Perspective

Lattanzio

Cardinali

Linsalata

2012

Recent Advances in Polyphenol Research

103

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Gene Expression Profiling in Response to Ultraviolet Radiation in Maize Genotypes with Varying Flavonoid Content

Cited by 207 publications

References 78 publications

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

Crosslinking of Ribosomal Proteins to RNA in Maize Ribosomes by UV-B and Its Effects on Translation

Plant Phenolics: A Biochemical and Physiological Perspective

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