Gene expression profiling in Ishikawa cells: A fingerprint for estrogen active compounds

Boehme, Kathleen; Simon, Stephanie; Mueller, Stefan O.

doi:10.1016/j.taap.2009.01.006

Cited by 33 publications

(21 citation statements)

References 85 publications

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“…In the experiments with gram (Cicer arietinum L.) and mustard (Brassica juncea L.) it was discovered that seed incubation caused after-effects such as a strong inhibition in the synthesis of chlorophylls a, b and carotenoids (Kumar and Sinha 1995) and such a response was explained as a result of the disturbance in the pigment synthesis by restricting the growth hormone-induced synthesis of RNA, DNA and proteins in the leaf. Similarly, strong metabolism responses to ZEN were also observed in animals (Mueller et al 2004, Boehme et al 2009). …”

Section: Photochemical Activity Of Psiimentioning

confidence: 63%

The effect of zearalenone on PSII photochemical activity and growth in wheat and soybean under salt (NaCl) stress

et al. 2011

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The effects of mycotoxin zearalenone (ZEN) on the photochemical activity of photosystem II (PSII) in wheat and soybean leaf discs incubated in ZEN solutions as well as the after-effects of pre-sowing soaking of seeds in solutions containing ZEN on the photochemical activity of PSII and on the seedlings growth under salt stress (NaCl solutions were investigated). The incubation of wheat leaf discs in ZEN solutions strongly inhibited the energy flux per cross section (CS) for absorption (ABS/CS), trapping (TRo/CS) and electron transport (ETo/CS), while the effects of ZEN action on soybean discs were opposite and the values of those parameters significantly increased with the increase in ZEN concentration. Incubation of seeds in a ZEN solution resulted in an increase in photochemical efficiency of PSII in soybean seedlings, but did not induce any response of PSII in those of wheat at medium illuminations. Only at the stronger illumination for both species did ZEN induce an increase in efficiency of excitation energy capture by open PSII reaction centers, photochemical quenching of chlorophyll a fluorescence and quantum yield of PSII electron transport. Pre-sowing soaking of seeds in a ZEN solution decreased the photoinhibitory injuries of PSII in wheat and soybean due to safe scattering of the excess excitation energy through an increase in energy-dependent quenching (qE) and state transition quenching (qT). ZEN when added to NaCl solutions during the period of germination contributed to reduction in the growth inhibition of wheat seedlings. The incubation of wheat leaf discs in ZEN solutions strongly inhibited CS, ABS/CS, TRo/CS and ETo/CS. Possible effects of ZEN on some physiological processes in plants have been discussed especially in the context with photochemical activity of PSII and a salt stress.

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Section: Photochemical Activity Of Psiimentioning

confidence: 63%

The effect of zearalenone on PSII photochemical activity and growth in wheat and soybean under salt (NaCl) stress

et al. 2011

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“…p21, the cdk inhibitor, disrupts the formation of the cyclin-cdk complex and inhibits G1/S transition (34,35). E2 is reported to stimulate cellular proliferation, PR expression and morphological differentiation in the endometrial Ishikawa cancer cell line by stimulating the transition of cell cycle via upregulation of cyclin D and cyclin A and downregulation of p21, p27 and p53 (36,37). Our previous results also showed that the expression levels of the cyclin D and cdk-4 genes in the BG-1 cell line were significantly increased with E2 (10 -7 M) or BPA (10 -5 M) treatment for 24 h (2).…”

Section: Discussionmentioning

confidence: 99%

Cell growth of BG-1 ovarian cancer cells is promoted by di-n-butyl phthalate and hexabromocyclododecane via upregulation of the cyclin D and cyclin-dependent kinase-4 genes

Hwang

Lee

et al. 2011

Mol Med Report

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Abstract. Endocrine-disrupting chemicals (EDCs) are environmentally persistent exogenous compounds released from various industrial products such as plastics, pesticides, drugs, detergents and cosmetics. They can cause a variety of adverse effects to the reproductive, developmental, immune and nervous systems in humans and wildlife. Di-n-butyl phthalate (DBP) is the main compound of phthalates and is reported to inhibit estrogen receptor (ER)-mediated gene expression and to interfere with normal fetal development of the male reproductive system. Hexabromocyclododecane (HBCD or HBCDD) is one of the brominated flame retardants (BFRs) which have been widely used in plastic, electronic and textile applications and are known to cause endocrine disruption with toxicity of the nervous system. In the present study, the estrogenic effects of DBP and HBCD were examined in an ovarian cancer cell line, BG-1, expressing high levels of ER via MTT assay and semi-quantitative reverse-transcription PCR. Treatment with DBP (10 -8 -10 -5 M) or HBCD (2x10 -8 -2x10 -6 M) resulted in increased cell proliferation of BG-1 cells as observed with 17-β estradiol (E2). In addition, both DBP and HBCD upregulated the expression levels of cell cycle-regulatory genes, such as cyclin D and cyclin-dependent kinase-4 (cdk-4), which are downstream target genes of ER, at 6 h after treatment. However, the expression of the p21 gene was not altered by DBP or HBCD at any time as with E2. Taken together, these results suggest that DBP and HBCD are EDCs which have apparent estrogenic activities by stimulating the cell proliferation of BG-1 cells and by inducing the expression of cyclin D and cdk-4. Our results suggest that DBP and HBCD have sufficient potency to disrupt the endocrine system and to stimulate cell growth in ER-positive cancer cells.

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“…Fulvestrant was also capable of blocking ERmediated gene regulation in response to various other estrogenic compounds (e.g. genistein, resveratol) in endometrial cancer cells (147). Microarray expression analysis of cells after treatment with estradiol, raloxifene, tamoxifen, or fulvestrant revealed that the ER ligands…”

Section: Studies Of Fulvestrant In Ishikawa Cells Have Yielded Mixed mentioning

confidence: 99%

Treating gynecologic malignancies with selective estrogen receptor downregulators (SERDs): promise and challenges

Boisen

Andersen

Sreekumar

et al. 2015

Molecular and Cellular Endocrinology

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Gene expression profiling in Ishikawa cells: A fingerprint for estrogen active compounds

Cited by 33 publications

References 85 publications

The effect of zearalenone on PSII photochemical activity and growth in wheat and soybean under salt (NaCl) stress

The effect of zearalenone on PSII photochemical activity and growth in wheat and soybean under salt (NaCl) stress

Cell growth of BG-1 ovarian cancer cells is promoted by di-n-butyl phthalate and hexabromocyclododecane via upregulation of the cyclin D and cyclin-dependent kinase-4 genes

Treating gynecologic malignancies with selective estrogen receptor downregulators (SERDs): promise and challenges

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