Gene expression profiling in AML with normal karyotype can predict mutations for molecular markers and allows novel insights into perturbed biological pathways

High expression of lymphoid enhancer-binding factor-1 (LEF1) is a novel favorable prognostic factor in cytogenetically normal acute myeloid leukemia

“…19 In the independent validation cohort provided by the AMLSG, pretreatment BM (n ϭ 142) or peripheral blood (n ϭ 54) samples were studied by independent investigators using cDNA microarrays (n ϭ 130) or Affymetrix HG-U133plus2.0 oligonucleotide microarrays (n ϭ 66), as previously described. 20,21 LEF1 expression levels were dichotomized at the median of all samples. Analysis of differentially expressed genes and gene set enrichment analysis (GSEA) are described in supplemental Methods.…”

Gene expression profiling in MDS and AML: potential and future avenues

“…With the notable exception of the MILE study, 16 so far there has been a relative paucity of other prospective and confirmatory studies, although recent studies performed within large AML study groups also suggest the potential clinical usefulness of GEP. 17,18 Although critics might state a lack of clear-cut advantages in comparison with standard cytogenetic and molecular genetic diagnostics, GEP-based diagnostic platforms might be useful for the identification of patients with masked fusion proteins (like t(15;17) and t(8;21)) that are not detected by conventional cytogenetics or fluorescence in situ hybridization. 97,98 In addition, GEP-based diagnostic platforms can provide information comparable to that of multiple different cytogenetic and molecular genetic analysis within a single experiment.…”

Comparing cancer vs normal gene expression profiles identifies new disease entities and common transcriptional programs in AML patients

“…In cancer, GEP has been used successfully to identify cancer subtypes, to stratify patients into responders vs nonresponders, and to predict survival but has, to a large extent, failed to uncover genes that are causally involved in cancer initiation and maintenance. [1][2][3][4][5][6][7][8] These genes are obviously of great interest because they constitute potential targets for therapeutic intervention.…”