Gene expression profiling for molecular distinction and characterization of laser captured primary lung cancers

Abstract

Methods: We examined gene expression profiles of tumor cells from 29 untreated patients with lung cancer (10 adenocarcinomas (AC), 10 squamous cell carcinomas (SCC), and 9 small cell lung cancer (SCLC)) in comparison to 5 samples of normal lung tissue (NT). The European and American methodological quality guidelines for microarray experiments were followed, including the stipulated use of laser capture microdissection for separation and purification of the lung cancer tumor cells from surrounding tissue.

Desmocollin-3 and Cancer

“…DSC3 is more specific for squamous NSCLC compared to p63 as p63 is also expressed in Adenocarcinoma. DSC3 gene is up-regulated in squamous NSCLC and down regulated in adenocarcinoma [43]. Specificity of DSC3 is 100% while sensitivity is variable [18-20, 44,45] and varies with differentiation of tumor.…”

Genetic and pharmacologic inhibition of EPHA2 promotes apoptosis in NSCLC

“…Genome-wide expression analyses of human lung cancer have identified a number of receptor tyrosine kinases (RTKs) as overexpressed and potentially representing drivers of non-small cell lung cancer (NSCLC) (1)(2)(3)(4). Among these RTKs was EPHA2, which belongs to the largest family of RTKs, the EPH family.…”

Genome-wide identification of cancer-related polyadenylated and non-polyadenylated RNAs in human breast and lung cell lines

“…However, for poly(A) DETs, the functions of these genes are associated with protein translation elongation; for example, ribosomal protein SA (RPSA) causes protein translation elongation and is upregulated in lung cancer [25,26]. EIF4EBP1, a repressor of translation with a short poly(A) tail, is overexpressed in patients with lung cancer [27,28]. EIF4EBP1 directly interacts with eukaryotic translation initiation factor 4E (eIF4E) to inhibit protein translation complex assembly and repress translation.…”