Gene expression profiling and antigen mining of the tuberculin production strain Mycobacterium bovis AN5

Pelayo, M. Carmen Garcia; Golby, Paul; Pirson, Chris; Ewer, Katie; Vordermeier, Martin; Hewinson, R. Glyn; Gordon, Stephen V.

doi:10.1016/j.vetmic.2008.07.004

Cited by 5 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). Overall, all MBE field isolates clustered with M. bovis AN5 originally a Brazilian strain used to produce PPD worldwide 25,26 while the BCG-Like isolates clustered with Russian BCG strain which included the deletion of the RD1 region 19 (Supplemental Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

Genomic Polymorphism Associated with the Emergence of Virulent Isolates of Mycobacterium bovis in the Nile Delta

Abdelaal

Spalink

Amer³

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Mycobacterium bovis is responsible for bovine tuberculosis in both animals and humans. Despite being one of the most important global zoonotic disease, data related to the ecology and pathogenicity of bovine tuberculosis is scarce, especially in developing countries. In this report, we examined the dynamics of M. bovis transmission among dairy cattle in the Nile Delta of Egypt. Animals belonging to 27 herds from 7 governorates were tested by the Single Intradermal Comparative Skin Tuberculin (SICST), as a preliminary screen for the presence of bovine tuberculosis. Positive SICST reactors were identified in 3% of the animals spread among 40% of the examined herds. Post-mortem examination of slaughtered reactors confirmed the presence of both pulmonary and/or digestive forms of tuberculosis in > 50% of the examined animals. Targeted and whole-genome analysis of M. bovis isolates indicated the emergences of a predominant spoligotype (SB0268) between 2013–2015, suggesting a recent clonal spread of this isolate within the Nile Delta. Surprisingly, 2 isolates belonged to M. bovis BCG group, which are not allowed for animal vaccination in Egypt, while the rest of isolates belonged to the virulent M. bovis clonal complex European 2 present in Latin America and several European countries. Analysis of strain virulence in the murine model of tuberculosis indicated the emergence of a more virulent strain (MBE4) with a specific genotype. More analysis is needed to understand the molecular basis for successful spread of virulent isolates of bovine tuberculosis among animals and to establish genotype/phenotype association.

show abstract

Section: Resultsmentioning

confidence: 99%

Genomic Polymorphism Associated with the Emergence of Virulent Isolates of Mycobacterium bovis in the Nile Delta

Abdelaal

Spalink

Amer³

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Expression differences between wild-type and attenuated vaccine strains of M. bovis have been reported, and it is thought that these differences may have a major impact on antigenic profiles (5). Mycobacterial gene expression work to date has been largely performed using platform technologies like microarrays, and while this approach provides an indication of large global expression changes, it lacks specificity for particular pathways postulated to contribute to virulence (25).…”

mentioning

confidence: 99%

Comparative Transcriptional Study of the Putative Mannose Donor Biosynthesis Genes in Virulent Mycobacterium tuberculosis and Attenuated Mycobacterium bovis BCG Strains

et al. 2011

View full text Add to dashboard Cite

Mycobacterium tuberculosis contains mannosylated cell wall components which are important in macrophage recognition and response. The building block for the mannosyl constituents of these components is GDP-mannose, which is synthesized through a series of enzymes involved in the mannose donor biosynthesis pathway. Nothing is known about the expression levels of the genes encoding these enzymes during the course of infection. To generate transcriptional profiles for the mannose donor biosynthesis genes from virulent M. tuberculosis and attenuated Mycobacterium bovis BCG, bacteria were grown in broth culture and within human macrophages. Our results with broth-grown bacteria show that there are differences in expression of the selected genes between M. tuberculosis and BCG, with increased expression of manC in M. tuberculosis and manA in BCG during stationary-phase growth. Results for M. tuberculosis extracted from within macrophages show that whiB2 is highly expressed and manB and manC are moderately expressed during infection. Rv3256c, Rv3258c, and ppm1 have high expression levels early and decreased expression as the infection progresses. Results with BCG show that, as in M. tuberculosis, whiB2 is highly expressed throughout infection, whereas there is either low expression or little change in expression of the remaining genes studied. Overall, our results show that there is differential regulation of expression of several genes in the mannose donor biosynthesis pathway of M. tuberculosis and BCG grown in broth and within macrophages, raising the possibility that the level of mannose donors may vary during the course of infection and thereby impact the biosynthesis of mannose-containing cell wall molecules.Tuberculosis (TB) kills nearly 2 million people each year and has become the leading cause of death among HIV patients. Although treatments have been available for more than 80 years, inconsistent completion of antibiotic courses has led to resistance to all current anti-TB drugs among bacterial isolates (33). The emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of TB, especially in HIV patients, poses a serious threat to the control of TB worldwide. Mycobacterium tuberculosis is the causative agent of TB and possesses a compositionally unique cell wall, which is the target of several TB therapeutics, like isoniazid and ethambutol. The last time a new drug was marketed for TB was 1963 (12), and coupled with the development of resistance, this lack of newer drugs emphasizes a critical need for the discovery of new drug targets in M. tuberculosis.It has long been thought that M. tuberculosis has coevolved with its human host, which is the only known reservoir (29). The complex pathogenicity of this bacterium in the context of its preferred niche is only partially understood. Comparative genomics between virulent strains of M. tuberculosis and the attenuated vaccine strain Mycobacterium bovis BCG have revealed gene deletions in BCG, providing insight into some key determinants ...

show abstract

Expression and purification of diagnostically sensitive mycobacterial (Mycobacterium bovis) antigens and profiling of their humoral immune response in a rabbit model

Kwok¹,

Scott

Snoddy³

et al. 2010

Research in Veterinary Science

View full text Add to dashboard Cite

Gene expression profiling and antigen mining of the tuberculin production strain Mycobacterium bovis AN5

Cited by 5 publications

References 17 publications

Genomic Polymorphism Associated with the Emergence of Virulent Isolates of Mycobacterium bovis in the Nile Delta

Genomic Polymorphism Associated with the Emergence of Virulent Isolates of Mycobacterium bovis in the Nile Delta

Comparative Transcriptional Study of the Putative Mannose Donor Biosynthesis Genes in Virulent Mycobacterium tuberculosis and Attenuated Mycobacterium bovis BCG Strains

Expression and purification of diagnostically sensitive mycobacterial (Mycobacterium bovis) antigens and profiling of their humoral immune response in a rabbit model

Contact Info

Product

Resources

About